Enzyme microsensor for glucose with an electrochemically synthesized enzyme-polyaniline film

Shinohara, Hiroaki; Chiba, Tsuneo; Aizawa, Mazuo

doi:10.1016/0250-6874(88)85031-5

Cited by 230 publications

(41 citation statements)

References 6 publications

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“…Based on the decrease of electrocatalytic response, glucose can be determined. This method is different from first -generation glucose biosensor, on which the determination is realized by monitoring either the consumption of oxygen [25], or the production of hydrogen peroxide [26 -27]. The latter requires a high anodic potential, thus results in interference of oxidizable substances such as ascorbic and uric acid.…”

Section: Introductionmentioning

confidence: 99%

Unadulterated Glucose Biosensor Based on Direct Electron Transfer of Glucose Oxidase Encapsulated Chitosan Modified Glassy Carbon Electrode

et al. 2008

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Glucose oxidase (GOD) was encapsulated in chitosan matrix and immobilized on a glassy carbon electrode, achieving direct electron transfer (DET) reaction between GOD and electrode without any nano-material. On basis of such DET, a novel glucose biosensor was fabricated for direct bioelectrochemical sensing without any electron-mediator. GOD incorporated in chitosan films gave a pair of stable, well-defined, and quasireversible cyclic voltammetric peaks at about À 0.284 (E pa ) and À 0.338 V (E pc ) vs. Ag/AgCl electrode in phosphate buffers. And the peak is located at the potentials characteristic of FAD redox couples of the proteins. The electrochemical parameters, such as midpoint potential (E 1/2 ) and apparent heterogeneous electron-transfer rate constants (k s ) were estimated to À 0.311 V and 1.79 s À1 by voltammetry, respectively. Experimental results indicate that the encapsulated GOD retains its catalytic activity for the oxidation of glucose. Such a GOD encapsulated chitosan based biosensor revealed a relatively rapid response time of less than 2 min, and a sufficient linear detection range for glucose concentration, from 0.60 to 2.80 mmol L À1 with a detection limit of 0.10 mmol L À1 and electrode sensitivity of 0.233 mA mmol À1. The relative standard deviation (RSD) is under 3.2% (n ¼ 7) for the determination of practical serum samples. The biologic compounds probably existed in the sample, such as ascorbic acid, uric acid, dopamine, and epinephrine, do not affect the determination of glucose. The proposed method is satisfactory to the determination of human serum samples compared with the routine hexokinase method. Both the unique electrical property and biocompatibility of chitosan enable the construction of a good bio-sensing platform for achieved DET of GOD and developed the third-generation glucose biosensors.

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Section: Introductionmentioning

confidence: 99%

Unadulterated Glucose Biosensor Based on Direct Electron Transfer of Glucose Oxidase Encapsulated Chitosan Modified Glassy Carbon Electrode

et al. 2008

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“…In this study, our results deqonstrate the feasibility of using PA-coated electrodes as a potentiometric sensor for dissolved oxygen in solutions and that such a sensor can be fabricated straightforwardly in a miniaturized form [17]. Furthermore, it is possible to modify polymer films so that oxygen-generating catalysts/enzymes can be impregnated and followed by potentiometric sensing of such a material [36].…”

Section: Resultsmentioning

confidence: 56%

Electrochemistry of conductive polymer X: Polyaniline‐based potentiometric sensor for dissolved oxygen

1991

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A platinum electrode coated with plyaniline (PA) was examined as a potentiometric or an optical .sensor for dissolved oxygen, and the results are reported. While the polymer-modified electrode behaved satisfactorily a s a potentiometric sensor, i Ls optical response (i.e., absorbance) gave a narrow linear region for varied oxygen concentrations. This conducting polymer-coated electrode was characterized to give approximately 97 mV/decade of the oxygen concentration and a voltage signal range of about 400 mV, representing at least three orders of magnitudes of dynamic linearity over the dissolved oxygen concentration. The potentiometric sensing electrode showed reasonably short response times, excellent reproducibility, and good stability. A detection limit of about 5 X lo-' mol/L of dissolved oxygen was estimated from the potentiometric response of PA-coated electrodes. ZNTRODUCTIONOxygen sensors have received a great deal of interest due to their importance in analyzing oxygen in industrial, environmental, and medical applications [ 1-41. Oxygen sensors can be classified broadly into three categories:( 1 ) a solid-state type operating normally at high temperatures [ 11, which includes solid electrolytes such as calciadoped zirconia and semiconductors such as titania or other metal oxides; ( 2 ) fluoroescence or phosphorescence sensors [2], which utilize the ability of oxygen to quench fluorescence or phosphorescence of polycyclic aromatic hydrocarbons such a s 9,lO-diphenylanthracene or pyrene derivatives; and ( 3 ) electrochemical sensors [3,4], which usually measure the faradaic current for oxygen reduction. The first two categories of sensors are suitable for quantitating oxygen in a gas mixture at various temperatures, while the electrochemical sensor is generally used for the dissolved oxygen analysis.The importance of analysis of dissolved oxygen in various liquids is well summarized by Hitchman To whom correspondence should be addressed. electrode (MPOE) [ 4 ], requires that both the ca'thode and anode be sealed by a membrane through which oxygen permeates and is reduced at the cathode. Thus, selection of materials for a membrane as well a s a working electrode constitutes a major problem for the Clark cell. Due to the irreversible nature of the oxygen reduction at almost any electrode, properly chosing the electrode material is very important. Gold is regarded a s the best electrode material because of its low adsorption of oxygen and of the high overpotential for the formation of its oxide [4]. The loss of electrolyte through the membrane is another problem. Kecently, silver has also been used as a cathode along with an Ag/AgCI anode/reference electrode [ 5 ] . Polyaniline (PA) has been studied extensively as one of conducting polymers due primarily to its straightforward preparation methods and the stabihy of its doped state under ambient experimental conditions [6-231. During the course of these studies, we [24-291 discovered that PA has four different oxidation states depending on the potenti...

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“…Mediators can be immobilized simultaneously with the enzyme during electropolymerization [68,75,[77][78][79], but they can also be conjugated with the monomer before polymerization [80]. This well controlled procedure of enzyme immobilization by electropolymerization is of great signficance in fabrication of microsensors [16,49,[78][79][80][81][82][83][84], in preparation of multilayer devices [78,85,86] and multienzyme biosensors [67,81,[87][88][89].…”

Section: Immobilization Of Enzymes In the Polymer Layermentioning

confidence: 99%

“…Several papers were also devoted to the use of poly(Nmethylpyrrole) [52,74,75,111,112] and a theoretical model of such a sensor operation was developed [112]. Other studies on glucose oxidase immobilization dealt with conducting polyaniline [16,44,85,113] and several non-conducting polymers, e.g. poly(o-phenylenediamine) [24][25][26][27]114 A list of other polymers successfully immobilized in electrodeposited polymer films includes various oxidases and dehydrogenases (Table t) and again polypyrrole is the most widely used as a matrix for their immobilization.…”

Section: Immobilization Of Enzymes In the Polymer Layermentioning

confidence: 99%

Electrochemical biosensors based on enzymes immobilized in electropolymerized films

Trojanowicz

Krawczyk

1995

Mikrochim Acta

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Enzyme microsensor for glucose with an electrochemically synthesized enzyme-polyaniline film

Cited by 230 publications

References 6 publications

Unadulterated Glucose Biosensor Based on Direct Electron Transfer of Glucose Oxidase Encapsulated Chitosan Modified Glassy Carbon Electrode

Unadulterated Glucose Biosensor Based on Direct Electron Transfer of Glucose Oxidase Encapsulated Chitosan Modified Glassy Carbon Electrode

Electrochemistry of conductive polymer X: Polyaniline‐based potentiometric sensor for dissolved oxygen

Electrochemical biosensors based on enzymes immobilized in electropolymerized films

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