Enzyme-Mimetic Activity of Ce-Intercalated Titanate Nanosheets

Kamada, Kei; Soh, Nobuaki

doi:10.1021/jp512038x

Cited by 18 publications

(13 citation statements)

References 36 publications

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“…Of the three NPs tested for surface reactivity, NPs of Y 2 O 3 emerge with highest surface reactivity. The ROS scavenging capacity plays critical role in the antioxidant activity reported for CeO 2 NPs [23,24]. Several reports have shown NPs of Y 2 O 3 and CeO 2 with similar characteristics to offer protection against induced toxicity by pro-oxidants [9,22].…”

Section: Discussionmentioning

confidence: 99%

“…Redox active NPs such as CeO 2 are known to possess reactive oxygen species (ROS) scavenging capability. This ROS scavenging capacity plays critical role in the antioxidant activity reported of these NPs, though other factors might also exist [23,24]. In this study, SOD-like and CAT-like activity of Y 2 O 3 NPs was measured along with NPs of CeO 2 and ZnO.…”

Section: Y 2 O 3 Nps Tem Sizes Hydrodynamic Sizes and Zeta Potentialmentioning

confidence: 91%

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High Surface Reactivity and Biocompatibility of Y2O3 NPs in Human MCF-7 Epithelial and HT-1080 Fibro-Blast Cells

et al. 2020

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This study aimed to generate a comparative data on biological response of yttrium oxide nanoparticles (Y2O3 NPs) with the antioxidant CeO2 NPs and pro-oxidant ZnO NPs. Sizes of Y2O3 NPs were found to be in the range of 35±10 nm as measured by TEM and were larger from its hydrodynamic sizes in water (1004 ± 134 nm), PBS (3373 ± 249 nm), serum free culture media (1735 ± 305 nm) and complete culture media (542 ± 108 nm). Surface reactivity of Y2O3 NPs with bovine serum albumin (BSA) was found significantly higher than for CeO2 and ZnO NPs. The displacement studies clearly suggested that adsorption to either BSA, filtered serum or serum free media was quite stable, and was dependent on whichever component interacted first with the Y2O3 NPs. Enzyme mimetic activity, like that of CeO2 NPs, was not detected for the NPs of Y2O3 or ZnO. Cell viability measured by MTT and neutral red uptake (NRU) assays suggested Y2O3 NPs were not toxic in human breast carcinoma MCF-7 and fibroblast HT-1080 cells up to the concentration of 200 μg/mL for a 24 h treatment period. Oxidative stress markers suggested Y2O3 NPs to be tolerably non-oxidative and biocompatible. Moreover, mitochondrial potential determined by JC-1 as well as lysosomal activity determined by lysotracker (LTR) remained un-affected and intact due to Y2O3 and CeO2 NPs whereas, as expected, were significantly induced by ZnO NPs. Hoechst-PI dual staining clearly suggested apoptotic potential of only ZnO NPs. With high surface reactivity and biocompatibility, NPs of Y2O3 could be a promising agent in the field of nanomedicine.

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Section: Discussionmentioning

confidence: 99%

Section: Y 2 O 3 Nps Tem Sizes Hydrodynamic Sizes and Zeta Potentialmentioning

confidence: 91%

High Surface Reactivity and Biocompatibility of Y2O3 NPs in Human MCF-7 Epithelial and HT-1080 Fibro-Blast Cells

et al. 2020

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“…These curves could be approximated by the typical sigmoid-function and a 50% inhibitory concentration of O2 -(IC50) of SOD was calculated. 29,30 The IC50 of SOD was successfully reduced from 0.170 to 0.036 U in the presence of TNS. The annihilation ability of SOD was elevated especially at low SOD concentrations less than 1 U.…”

Section: Please Do Not Adjust Marginsmentioning

confidence: 97%

Enhanced catalytic activity of enzymes interacting with nanometric titanate nanosheets

2015

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Coexistence effect of titanate nanosheets (TNS) with nanometric lateral dimensions (ca. 3 nm), which were prepared through a hydrolysis reaction of titanium tetraisopropoxide, on catalytic activity of horseradish peroxidase (HRP) was investigated as a function of solution pH. Especially in diluted HRP solutions with a pH range of 7 ~ 8, enzymatic reaction rate, i.e., maximum velocity (Vmax) in the cconventional Michaelis-Menten equation, was significantly enhanced more than 2 times in the presence of TNS. In contrast, the zincrease in Vmax was not very large in acidic (pH = 4.0) and basic solutions (pH = 9.0). It was demonstrated that the TNS brought about peptization of aggregates composed of several HRP molecules in a diluted solution, causing increase in an apparent HRP concentration participating in the enzymatic reaction. Moreover, the coexistent TNS activated superoxide dismutase (SOD) with O2 -scavenging performance.

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“…Naturally, SOD is believed to eliminate the anion which is superoxide O 2 − , ROS, throughout the dismutation response of O 2 − to hydrogen peroxide and oxygen. For surviving constraints linked with SOD, which is common, an assortment linked with nanomaterials is utilized to imitate SOD [116][117][118][119][120][121][122][123][124].…”

Section: Superoxide Dismustase (Sod) Mimicsmentioning

confidence: 99%

Potentiality of Nanoenzymes for Cancer Treatment and Other Diseases: Current Status and Future Challenges

et al. 2021

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Studies from past years have observed various enzymes that are artificial, which are issued to mimic naturally occurring enzymes based on their function and structure. The nanozymes possess nanomaterials that resemble natural enzymes and are considered an innovative class. This innovative class has achieved a brilliant response from various developments and researchers owing to this unique property. In this regard, numerous nanomaterials are inspected as natural enzyme mimics for multiple types of applications, such as imaging, water treatment, therapeutics, and sensing. Nanozymes have nanomaterial properties occurring with an inheritance that provides a single substitute and multiple platforms. Nanozymes can be controlled remotely via stimuli including heat, light, magnetic field, and ultrasound. Collectively, these all can be used to increase the therapeutic as well as diagnostic efficacies. These nanozymes have major biomedical applications including cancer therapy and diagnosis, medical diagnostics, and bio sensing. We summarized and emphasized the latest progress of nanozymes, including their biomedical mechanisms and applications involving synergistic and remote control nanozymes. Finally, we cover the challenges and limitations of further improving therapeutic applications and provide a future direction for using engineered nanozymes with enhanced biomedical and diagnostic applications.

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Enzyme-Mimetic Activity of Ce-Intercalated Titanate Nanosheets

Cited by 18 publications

References 36 publications

High Surface Reactivity and Biocompatibility of Y2O3 NPs in Human MCF-7 Epithelial and HT-1080 Fibro-Blast Cells

High Surface Reactivity and Biocompatibility of Y2O3 NPs in Human MCF-7 Epithelial and HT-1080 Fibro-Blast Cells

Enhanced catalytic activity of enzymes interacting with nanometric titanate nanosheets

Potentiality of Nanoenzymes for Cancer Treatment and Other Diseases: Current Status and Future Challenges

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