ENZYME POLYMORPHISM IN POPULATIONS OF THE TSETSE FLY GLOSSINA PALLIDIPES IN KENYA

Abstract

Enzyme polymorphism was investigated by means of horizontal starch gel electrophoresis in samples of 8 populations of Glossina pallidipes Austen in Kenya. Polymorphism was demonstrated in 3 of the 11 enzyme systems studied: non‐specific esterases, aldehyde oxidase, and leucine aminopeptidase. The amount of genetic variation was low for all samples. The results are discussed in connection with the geographical distribution of the populations studied. It is concluded that the significance of the zymogram technique as used in this study, is limited for the characterization of populations of G. pallidipes. ZUSAMMENFASSUNG Enzympolymorphismus in Populationen der Tsetsefliege Glossina pallidipes Austen in Kenya Enzympolymorphismus wurde in Proben von 8 Populationen von Glossina pallidipes aus Kenya mit Hilfe horizontaler Stärkegelelektrophorese untersucht. Polymorphismus wurde in 3 der 11 untersuchten Enzymsysteme nachgewiesen: Nicht‐spezifische Esterase, Aldehydoxydase und Leucin‐Aminopeptidase. Der Umfang der genetischen Variation war in allen Proben gering. Die Resultate werden diskutiert in Zusammenhang mit der geographischen Verteilung der untersuchten Populationen. Offenbar ist die Bedeutung der Zymogrammtechnik. wie sie in dieser Untersuchung verwendet wurde, zur Charakterisierung von Populationen von G. pallidipes begrenzt.

Genetic and behavioural differences between Glossina pallidipes from Uganda and Zimbabwe

“…However, Gooding & Rolseth (1982) have shown the existence of considerable isoenzyme variation within the sub-species G. m. morsitans. Isoenzyme differences also occur between allopatric populations of G. pallidipes in Kenya ( Van Etten, 1982) and these are accompanied by differences in mating behaviour (Van Etten, 1981). The present study has shown that there are differences in mating behaviour between G. pallidipes of Zimbabwe and of Uganda origin in that Zimbabwe flies are less willing to mate under the same conditions in the laboratory.…”

“…Subsequently Jaenson (1978 a) reported a genetic basis for differences in copulation time between flies of allopatric populations in Kenya whilst Van Etten (1981) described differences in reproductive performance of laboratory colonies of G. pallidipes from two allopatric populations in Kenya and these populations were found to be polymorphic for a number of enzyme systems ( van Etten, 1982). Subsequently Jaenson (1978 a) reported a genetic basis for differences in copulation time between flies of allopatric populations in Kenya whilst Van Etten (1981) described differences in reproductive performance of laboratory colonies of G. pallidipes from two allopatric populations in Kenya and these populations were found to be polymorphic for a number of enzyme systems ( van Etten, 1982).…”

“…in Uganda. Subsequently Jaenson (1978 a) reported a genetic basis for differences in copulation time between flies of allopatric populations in Kenya whilst Van Etten (1981) described differences in reproductive performance of laboratory colonies of G. pallidipes from two allopatric populations in Kenya and these populations were found to be polymorphic for a number of enzyme systems (van Etten, 1982).…”

PRIMARY ATTRACTION OF MOUNTAIN PINE BEETLE, DENDROCTONUS PONDEROSAE HOPK. (COLEOPTERA: SCOLYTIDAE), TO BOLTS OF LODGEPOLE PINE

“…Genetic polymorphism has been demonstrated in Glossina morsitans morsitans Westwood by starch gel electrophoresis using whole flies (Van der Geest and Kawooya 1975; Van der Geest et al 1978) and by polyacrylamide gel electrophoresis using fly thoraces and midgut . Van Etten (1982) analyzed eight populations of Glossina pallidipes Austen in Kenya using starch gel electrophoresis and found that three (esterase, aldehyde oxidase, and leucine arninopeptidase) of the 11 enzymes examined showed polymorphism. However, he considered that the differences in allozyme frequencies were small and did not provide a suitable tool for population characterization.…”

A comparison of susceptibility of two allopatric populations of Glossina pallidipes for stocks of Trypanosoma congolense

“…Glossina pallidipes Austen is a vector of Trypanosoma congolense Broden infections for cattle in eastern Africa (Harley, 1967;England & Baldry, 1972;Wilson et al, 1972;Moloo et al, 1973;Snow & Tarimo, 1983). It has a wide distribution in Kenya (Ford & Katondo, 1977) where there are two major fly belts of this tsetse species: an eastern belt including the coastal area (Shimba Hills included), Kibwezi and Mara; and a western belt including Nguruman, Maasai Mara and Lambwe Valley ( van Etten, 1982). In the present study, the vector competence of laboratory-reared G.pallidipes originating from these two allopatric populations in Kenya, for T.congolense stocks isolated from G.pallidipes in Nguruman and Shimba Hills, is compared.…”