Enzyme production and oil palm empty fruit bunch bioconversion to ethanol using a hybrid yeast strain

Abstract

Oil palm empty fruit bunch (OPEFB) is a lignocellulosic biomass generated in palm oil mills. It is a sustainable resource for fuels and chemicals. In this study, OPEFB was converted to ethanol by an integrative OPEFB conversion process including dilute alkaline pretreatment, cellulolytic enzyme production, separate OPEFB hydrolysis, and cofermentation using a hybrid xylose-fermenting yeast. OPEFB was pretreated using 1% (w/v) NaOH solution followed by 1% (v/v) H 2 O 2. Further, cellulolytic enzymes were produced by submerged fermentation using Trichoderma reesei Rut C30 and used for OPEFB hydrolysis. The filter paper cellulase activity of the crude cellulolytic enzymes was 15.1 IU/mL, which was higher than those obtained by reported Trichoderma strains under laboratory conditions. Glucose and xylose yields reached 66.9% and 74.2%, respectively, at 30 filter paper unit (FPU)/g-biomass enzyme dosage and 10% (w/v) biomass loading. The hybrid yeast strain ScF2 was previously constructed through recursive genome shuffling of Pichia stipitis and Saccharomyces cerevisiae and was used in OPEFB hydrolysate fermentation. About 16.9 g/L ethanol was produced with an ethanol yield of 0.34 g/g sugars, which was 67% of theoretical ethanol yield.

Black Soldier Fly (Hermetia Illucens) Sebagai Bioreaktor Alami Untuk Produksi Pupuk Organik Berbasis Limbah Pabrik Minyak Kelapa Sawit

“…Proses persiapan yang efektif sangat d i b u t u h k a n u n t u k m e m p e r c e p a t p r o s e s pengomposan untuk mencapai kualitas kompos TKKS yang diharapkan (Hamzah et al, 2018;Himmatun Wisda et al, 2016;Zulkiple et al, 2016). Persiapan TKKS dapat dilakukan dengan metode fisika, kimia, fisika-kimia, maupun biologis (Anita et al, 2020;Hamzah et al, 2018;Hidayat, 2013;Isroi et al, 2012;Muhd Ali et al, 2016;Zahrim et al, 2017;Zhai et al, 2020 Dalam proses pengomposan, diperlukan metode untuk menurunkan rasio C/N untuk meningkatkan kandungan nutrisi yang dapat diserap oleh tanaman. Tingginya rasio C/N disebabkan oleh tingginya kandungan C-organik yang diakibatkan oleh tingginya kandungan gula kompleks (Tahir et al, 2019).…”

Biofuels Production from Renewable Resources

“…Zhai et al. [1] demonstrate the feasibility of an innovative integrative process that includes dilute alkaline pretreatment, cellulolytic enzyme production, OPEFB hydrolysis, and cofermentation with a hybrid xylose‐fermenting yeast. Their work opens new perspectives for the use of OPEFB biomass to produce ethanol.…”

Integrated Production of Xylitol, Ethanol, and Enzymes from Oil Palm Empty Fruit Bunch through Bioprocessing as an Application of the Biorefinery Concept

“…Enzyme synthesis can be accomplished through fermentation by microbial organisms, often bacteria or fungi [12]. It has been shown that fungi exhibit a greater hydrolysis rate in comparison to yeast and bacteria.…”