Enzyme Systems of Stylonychia pustulata. II. Miscellaneous Systems (Hydrases, Hydrolases, and Dehydrogenases)

Abstract

SYNOPSIS. Histochemical techniques were used to demonstrate the intracellular distribution of some hydrases, hydrolases, oxidases, and dehydrogenases in Stylonychia pustulata. The hydrase, aconitase, was confined to the mitochondria. Zymohexase activity occurred in the cytoplasm and probably in the mitochondria. The hydrolases, acid and alkaline phosphatases, lipase, and urease, were localized in the mitochondria. Lactic and glutamic dehydrogenases were confined to the mitochondria. Peroxidase and glycerophosphate dehydrogenase were absent.

Histochemical Demonstration of DPN-Linked Dehydrogenases in <i>Trichomonas vaginali</i><i>s</i>

“…Hunter (1955) de monstrated activity of both the above dehydrogenase in the cyto plasm of Opalina carolinensis. Glycerophosphate dehydrogenase was found to be absent and lactic dehydrogenases located in the mitochondria and in the numerous granules beneath the mem brane of the macronuclei in Stglonychia pustulata (Hunter, 1959). The presence of a-glycerophosphate dehydrogenase has been in dicated in other trichomonads also (Lindblom, 1961).…”