Enzyme-Treated Zizania latifolia Ethanol Extract Protects from UVA Irradiation-Induced Wrinkle Formation via Inhibition of Lysosome Exocytosis and Reactive Oxygen Species Generation

Abstract

Ultraviolet A (UVA) is a risk factor for photoaging and wrinkle formation. Zizania latifolia is an herbaceous perennial plant. It contains many bioactive compounds such as tricin that show antioxidative and anti-inflammatory effects. The aim of this study was to investigate the antiwrinkle effect of a mixture of hydrolytic enzyme (cellulase, hemicellulase and pectinase)-treated Z. latifolia extract (ZLE) and tricin on UVA-irradiated human dermal fibroblasts (HDFs) and SKH-1 hairless mice. Treatment of UVA-irradiated HDF cells with ZLE and tricin significantly decreased UVA induced-plasma membrane rupture, generation of ROS, expression levels of total and secreted lysosomal associated membrane protein (LAMP-1), cathepsin B and metalloproteinases (MMPs) and inhibited NF-κB activation. In the animal study, UVA-damaged epidermal and dermal tissues were repaired by the ZLE and tricin treatments. Administration of ZLE or tricin to UVA-irradiated animals recovered skin surface moisture and collagen fiber in dermal tissue. Treatment of ZLE or tricin decreased wrinkle formation, secretion of MMPs and expression levels of vascular endothelial growth factor (VEGF) and cathepsin B, and increased the expression level of collagen-1 in UVA-irradiated animals. Overall, the ZLE and tricin treatments decreased the skin damage induced by UVA irradiation via inhibition of lysosomal exocytosis and ROS generation. Therefore, ZLE and tricin are promising as antiwrinkle and antiphotoaging agents.

Dermatological uses of rice products: Trend or true?

“…[25][26][27][28] These experiments have demonstrated tricin to be protective against skin damage caused by UVA radiation and UVBmediated photoaging. [26][27][28] Another study found tricin to inhibit IgEmediated allergic reactions. 25 Although the literature on biological activities of tricin in conjunction with momilactones is limited, research suggests that momilactones A and B and tricin isolated from rice husk all exhibit antiaging effects.…”

“…Numerous studies have investigated tricin extracted from enzyme‐treated Zizania latifolia , a wild rice 25–28 . These experiments have demonstrated tricin to be protective against skin damage caused by UVA radiation and UVB‐mediated photoaging 26–28 . Another study found tricin to inhibit IgE‐mediated allergic reactions 25 .…”

“…In experiments, tricin‐rich rice hull extracts were found to be anti‐inflammatory and antiadipogenic 24 . Numerous studies have investigated tricin extracted from enzyme‐treated Zizania latifolia , a wild rice 25–28 . These experiments have demonstrated tricin to be protective against skin damage caused by UVA radiation and UVB‐mediated photoaging 26–28 .…”

Flavonoids Isolated from the genus Ficus and Their Biological Activities

“…[61] The flavonoid tricin (116) was shown to improve the cutaneous system by regulating anti-inflammatory signaling pathways. [62] In addition, taxifolin (125) showed a good anti-inflammatory effect and could inhibited HT22 cells apoptosis at concentration of 2.5 μm (83% cell survival). [63] Flavonoids…”

Hepatoprotective effect of water bamboo shoot (Zizania latifolia) extracts against acute alcoholic liver injury in a mice model and screening of bioactive phytochemicals

“…The WBS is usually regarded as an ancient herbal medicine according to the Chinese ancient medicine literature. Previous studies have demonstrated that WBS exhibits antioxidant and antiultraviolet activities (An et al, 2020;Wang et al, 2017). According to the Chinese ancient medicine literature, "Compendium of Materia Medica" has alcoholismremission and hepatoprotective effects (Li, 2003).…”