Enzymehistochemical analysis of 5'-nucleotidase positive mononuclear cells in lymphocytic submandibulitis in NOD mice.

Abstract

The localization of 5'-nucleotidase (5'NT) activity on mononuclear cells in the inflammatory focus of the submandibular gland of the adult female non-obese diabetic (NOD) mouse was investigated by an enzymehistochemical technique.At the light microscopical level, the majority of infiltrating mononuclear cells demonstrated intense reaction products on the surface and the reactive cells showed a tendency of forming groups at the center of the inflammatory focus.At the electron microscopical level, mononuclear cells of the NOD mouse could be roughly differentiated into two kinds of cells, those which demonstrated intense reaction products on the plasma membrane (5'NT positive) and those which demonstrated less or no reaction products (5'NT negative). In the acini or granular convoluted tuble (GCT) cells, which lie adjacent to the inflammatory focus, 5'NT positive mononuclear cells were observed and the morphological appearance of these mononuclear cells resembled lymphocytes.In the present study, the possible role and biological significance of 5'NT positive mononuclear cells in the inflammatory focus are discussed.

Ultracytochemical and biochemical investigations of alkaline phosphatase and 5'-nucleotidase activities in carcinogen-induced well-and poorly-differentiated adenocarcinoma of the rat stomach.

“…Actually, lead has several drawbacks in some instances, such as an inhibitory effect to the enzyme, nonenzymatic breakdown of substrate, and nonspecific deposits of lead or lead phosphate (9, 35,36). The only problem with the cerium-based techniques, however, is that the reaction product is not readily visible at the light microscope level (36,40). That the cerium-based technique failed to demonstrate ALPase activity in the present study was ascribed possibly not only to increased acidity (pH 7.4) to retain stability of cerium solubulized in the reaction mixture but also to some inhibitory effects of the cerium as a capture agent to tissue-unspecific type ALPase of the rat stomach.…”