Enzymelektroden

Scheller, Frieder W.; Pfeiffer, D.

doi:10.1002/zfch.19780180203

Cited by 27 publications

(2 citation statements)

References 128 publications

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“…The ®rst goal is only reached when reaction rates are much higher for the immobilized mediator with biocatalyst than for the cosubstrate with biocatalyst. An alternative approach to the use of such mediators consists in restricting the analyte (substrate) concentration within the reaction layer through an appropriate outer membrane, whose permeability strongly favours co-substrate transport [9,10].…”

Section: Commissions On Biophysical Chemistry and Electroanalytical Cmentioning

confidence: 99%

“…Nevertheless linear dynamic ranges may be large if the sensor response is controlled by the substrate diffusion through the membrane and not by the enzyme kinetics. This control is achieved by placing a thin outer membrane over a highly active enzyme layer [9,10]: the thinner the membrane, the shorter the biosensor response time. Furthermore, such a diffusional barrier also makes the sensor response independent of the amount of active enzyme present and improves the sensor response stability.…”

Section: Inner and Outer Membranesmentioning

confidence: 99%

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Electrochemical Biosensors: Recommended Definitions and Classification

Venot¹,

Tóth

Durst

et al. 2016

IUPAC Standards Online

197

235

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Republication or reproduction of this report or its storage and/or dissemination by electronic means is permitted without the need for formal IUPAC permission on condition that an acknowledgement, with full reference to the source along with use of the copyright symbol q, the name IUPAC and the year of publication are prominently visible. Publication of a translation into another language is subject to the additional condition of prior approval from the relevant IUPAC National Adhering Organization.

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Section: Commissions On Biophysical Chemistry and Electroanalytical Cmentioning

confidence: 99%

Section: Inner and Outer Membranesmentioning

confidence: 99%

Electrochemical Biosensors: Recommended Definitions and Classification

Venot¹,

Tóth

Durst

et al. 2016

IUPAC Standards Online

197

235

View full text Add to dashboard Cite

show abstract

Verfahren und Analysenautomat zur Bestimmung von Nährlösungskomponenten und Stoffwechselprodukten

Weise¹,

Kreibich²,

Scheller³

1987

Acta Biotechnologica

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X continuous flow system with an enzyme electrode and with a new type of measuring instrument, t h e BIOXY-Meter, is described as a n example for the determination of glucose. The BIOXTMeter combined with various biosensors may be used in biotechnology and in other fields for the solution of a number of analytical problems. Measuring principle is the reduction of oxygen. The peak of first derivation of the current-time-curve is proportional to oxygen consumption and to decrease of substrate concentration. Enzyme fixation with common adhesive and a device for automated saturation of measuring samples with air in the sampler are also described. With the apparatus 60 samples per hour are analyzed for glucose. The linear measuring range is 0.1 mMol to 1.5 mMol with a coefficient of variation of V k = 0.93 per cent. The described type of enzyme electrode is stable for 2 to 12 weeks or 1,000 to 5,000 measurements.

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