Enzymes and the Rate of Chemical Reactions

Gross, Lou; Beals, Monica; Harrell, Susan

doi:10.25334/q4nf2g

Cited by 2 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Passive diffusion or permeation of small non-charged compounds can be regarded as conforming to a modified version of Fick's law and can be described by first-order kinetics. [29] Under the assumption that each analyte molecule entering a vesicle would be taken up by a CB8•MDAP, thus giving a change in the fluorescence signal, and neglecting analyte molecules that might detach again after complexing with CB8•MDAP and that complexed analyte molecules cannot exit the vesicle again, this kinetic process can be effectively modeled by fitting exponential curves to obtain the observed permeation rate (k obs ). In our setup, we obtain k obs = 0.0067 min −1 .…”

Section: Monitoring Of Emission In Immobilized Vesiclesmentioning

confidence: 99%

Stability of Immobilized Chemosensor‐Filled Vesicles on Anti‐Fouling Polymer Brush Surfaces

Yang,

Xiao,

Yang

et al. 2024

Adv Materials Inter

View full text Add to dashboard Cite

The characterization of phospholipid membrane permeability for small molecules is crucial for many applications in drug discovery and biomedical research in general. Here, chemosensor‐laden vesicles offer an attractive platform for permeability assays. In this work, the stability of immobilized chemosensor‐filled vesicles is explored on anti‐fouling polymer brush surfaces for potential use in monitoring small molecule membrane permeability. The study focuses on the development of a method for immobilizing sensor‐loaded vesicles into arbitrary patterns on surfaces and characterizing their stability under changing temperatures and compositions. As substrate to enable intact, long‐term stable vesicle immobilization reactive polymer brushes with anti‐fouling properties are used. Utilizing microchannel cantilever spotting, biotin moieties are introduced on the polymer brush surface, enabling stable tethering of vesicles through streptavidin‐biotin interactions. The immobilized vesicles are monitored through fluorescence microscopy for their response to analytes under changing environmental parameters and vesicle composition. A higher stability of immobilized vesicles compared to free‐floating ones is observed, with permeability only at elevated temperatures. By tuning vesicle compositions, permeability at lower temperatures can be raised again. Overall, the study provides insights into a novel approach for vesicle immobilization, showcasing the potential of surface‐bound vesicles for applications in microfluidic systems and as biosensors in various assays.

show abstract

Section: Monitoring Of Emission In Immobilized Vesiclesmentioning

confidence: 99%

Stability of Immobilized Chemosensor‐Filled Vesicles on Anti‐Fouling Polymer Brush Surfaces

Yang,

Xiao,

Yang

et al. 2024

Adv Materials Inter

View full text Add to dashboard Cite

show abstract

Effect of Concentration of Pectinase on Clarification and Ascorbic Acid of Lemon Ginger Drink

Lusihanne

Herawati

2020

J. Akademika Kimia

View full text Add to dashboard Cite

Lemon ginger drink is a herbal drink which contains many antioxidant and ascorbic acid that good for health. However, the murky appearance of the lemon ginger drink causes a lack of consumer interest especially young people in consuming this beverage. The murky in the lemon ginger drink is due to pectin content. Therefore, clarification is needed with the aid of a pectinase enzyme. The concentration of pectinase used in this study were 0; 0.04; 0.06; 0.08; 0.1; 0.1; and 0.12%; then incubated at 500 oC temperatures for 4 hours. The results showed that the proper concentration of pectinase in the clarification of lemon ginger drink was 0.08%. The concentration of the pectinase does not affect the ascorbic acid level of the lemon ginger drink.

show abstract

Enzymes and the Rate of Chemical Reactions

Cited by 2 publications

References 0 publications

Stability of Immobilized Chemosensor‐Filled Vesicles on Anti‐Fouling Polymer Brush Surfaces

Stability of Immobilized Chemosensor‐Filled Vesicles on Anti‐Fouling Polymer Brush Surfaces

Effect of Concentration of Pectinase on Clarification and Ascorbic Acid of Lemon Ginger Drink

Contact Info

Product

Resources

About