2019
DOI: 10.1080/14789450.2019.1630275
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Enzymes and their turnover numbers

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Cited by 26 publications
(21 citation statements)
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“…Intracellular phenomena occur over a range of timescales (upper, green), from bacterial division times as fast as 10 minutes (Rocha, 2004) down to physico-chemical fluctuations on the order of femto- to nano-seconds. The residence times of ultra-weak protein dimers (lower, left) are comparable to the timescales of elementary chemical reactions (Gruebele and Zewail, 1990) and protein conformational fluctuations (Bredenbeck et al, 2003), whereas higher-order clusters can last an order of magnitude longer (lower, center), on par with enzyme-catalyzed reactions (Smejkal and Kakumanu, 2019). This ultra-weak clustering regime operates below the timescales of phase separated droplets (Molliex et al, 2015; Tang et al, 2021), cell signaling (Asthagiri and Lauffenburger, 2003), and percolated networks (Ramm et al, 2022; Zia et al, 2014) (lower, right), which are sufficiently durable to be measured in vivo .…”
Section: Resultsmentioning
confidence: 99%
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“…Intracellular phenomena occur over a range of timescales (upper, green), from bacterial division times as fast as 10 minutes (Rocha, 2004) down to physico-chemical fluctuations on the order of femto- to nano-seconds. The residence times of ultra-weak protein dimers (lower, left) are comparable to the timescales of elementary chemical reactions (Gruebele and Zewail, 1990) and protein conformational fluctuations (Bredenbeck et al, 2003), whereas higher-order clusters can last an order of magnitude longer (lower, center), on par with enzyme-catalyzed reactions (Smejkal and Kakumanu, 2019). This ultra-weak clustering regime operates below the timescales of phase separated droplets (Molliex et al, 2015; Tang et al, 2021), cell signaling (Asthagiri and Lauffenburger, 2003), and percolated networks (Ramm et al, 2022; Zia et al, 2014) (lower, right), which are sufficiently durable to be measured in vivo .…”
Section: Resultsmentioning
confidence: 99%
“…We found that PPIs at the ultra-weak threshold ( V 0 / k B T = 2.5) drive clusters that last an order of magnitude longer than in the hard-sphere, zero-attraction limit, with dissociation times on the order of 100 ns ( Figure 3B , center). Such ultra-weak clusters are sufficiently durable to facilitate the fastest known enzyme-catalyzed reactions (Smejkal and Kakumanu, 2019) (please see Discussion, Figure 5 ). However, co-localization is still transient enough to maintain molecular mobility and enable the suspension to reorganize, unlike in the moderate-affinity case.…”
Section: Resultsmentioning
confidence: 99%
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“…To scavenge excess ROS and maintain the intracellular homeostasis, bacteria have evolved to be armed with an array of strategies. Of these, catalase, an enzyme with a turnover of 2.8 × 10 6 molecules per second (33), very efficiently converts H 2 O 2 into O 2 and water. For this reason, visualization of oxygen bubbles in the presence of H 2 O 2 and Triton X-100 offers a simple method to quantify catalase activity (34).…”
Section: Introductionmentioning
confidence: 99%
“…To scavenge the excess ROS and maintain the intracellular homeostasis, bacteria have evolved to be armed with array of strategies. Of these, catalase, an enzyme with a turnover number of 2.8×10 6 molecules per second 33 , very efficiently converts H 2 O 2 into O 2 and water. For this reason, visualization of oxygen bubbles in the presence of H 2 O 2 and Triton-X offered a simple method to quantify catalase activity 34 .…”
Section: Introductionmentioning
confidence: 99%