Enzymes for second generation ethanol: exploring new strategies for the use of xylose

Novello, Márcia; Vilasboa, Johnatan; Schneider, Willian Daniel Hahn; Reis, Laísa dos; Fontana, Roselei Claudete; Camassola, Marli

doi:10.1039/c4ra00909f

Cited by 16 publications

(11 citation statements)

References 39 publications

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“…The enzymatic activities are expressed as units per gram (U.g -1 ) of dry medium. Determination of mycelial mass, total soluble protein and pH determination Mycelial mass was estimated with N-acetylglucosamine according to Novello et al (2014). The total soluble protein was determinaed according to Bradford (1976).…”

Section: Enzyme Assaysmentioning

confidence: 99%

Use of elephant grass (Pennisetum purpureum) as substrate for cellulase and xylanase production in solid-state cultivation by Penicillium echinulatum

Menegol

Scholl

Dillon

et al. 2017

Braz. J. Chem. Eng.

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-The high cost of the enzymes used for the hydrolysis of lignocellulosic biomass is one of the bottlenecks in the production of second-generation ethanol and the use of local biomass, such elephant grass, can help to reduce this cost. In this investigation elephant grass biomass was evaluated as a carbon and inducer source of production of cellulases and xylanases by the fungus Penicillium echinulatum strain 9A02S1 in solid-state cultivation. The highest filter paper activity (13.26 U.g -1 dry matter) and β-glucosidase activity (138.34 U.g -1 dry matter) were obtained on the fifth day of cultivation, in medium containing biomass pre-treated with sulfuric acid and untreated, respectively. The highest endoglucanase activity was 158.44 U.g -1 dry matter in the presence of elephant grass pre-treated with sulfuric acid. The xylanase activity was highest with medium that was formulated with 75% untreated elephant grass and 25% wheat bran (372.62 U.g -1 dry matter). In conclusion, the results showed that it is possible to obtain large amounts of cellulases and xylanases using a cosmopolitan and very low cost substrate such as elephant grass.

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Section: Enzyme Assaysmentioning

confidence: 99%

Use of elephant grass (Pennisetum purpureum) as substrate for cellulase and xylanase production in solid-state cultivation by Penicillium echinulatum

Menegol

Scholl

Dillon

et al. 2017

Braz. J. Chem. Eng.

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“…Besides, the chemical hydrolysis generates inhibitors of the subsequent ethanolic fermentation, such as furfural and hydroxymethylfurfural. On the other hand, enzymes represent the third highest cost of the E2G process, following capital and feedstock [15,16].…”

Section: Enzymatic Hydrolysis Of Biomass and Enzyme Marketmentioning

confidence: 99%

Is cellulase production by solid-state fermentation economically attractive for the second generation ethanol production?

2017

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The cost of enzymes for the enzymatic route of the second generation ethanol production is a crucial bottleneck to turn this process into a reality. Solid-state fermentation (SSF) is an environmentally friendly process of enzyme synthesis, although little is known about the costs associated to it. Therefore, this work analyzed economical scenarios of cellulase production by SSF in a pilot plant integrated to both a first and a second generation ethanol processes. The chosen substrate for the enzyme production was composed by sugarcane bagasse and wheat bran, cultivated by the thermophilic fungus Myceliophthora thermophila I-1D3b at 45 C during 96 h. The estimation of the most important economic indicators showed that the SSF process is economically attractive, due to its easy integration to the main process, and its revenue is up to four fold greater than electricity cogeneration. Economic indicators, such as the internal rate of return (IRR) and payback, were higher than those usually accepted by Brazilian investor in the sucro-energetic sector. Nevertheless, return on investment (ROI) was under than that recommended by the literature. The sensitivity analysis showed strong influence of the enzyme activity on the economic indicators, being the most important parameter for the project profitability.

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“…Growth determinations were performed by determining the amount of N-acetyl-D-glucosamine present in the mycelium and converted fungal biomass according to the method described by Novello et al (2014).…”

Section: Mycelial Mass Determinationmentioning

confidence: 99%

“…Additionally, this process has a great potential for reducing carbon dioxide emissions, thereby contributing to reducing global warming. However, to convert lignocellulosic biomass into biofuel, specifically ethanol, it is necessary to perform hydrolysis on the biomass, which requires enzymes, particularly cellulases and xylanases (Basso et al, 2014;Mabee et al, 2011;Reis et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Commercially, most cellulases and xylanases are produced by SC because the production factors are easier to control. However, the SC method can be complex because it involves mixing, aeration, and control and monitoring of temperature, pH, dissolved oxygen and other factors (dos Reis et al, 2014;Reis et al, 2015), and the SC method requires specific equipment. Using SSC for enzyme production has attracted interest because it is a lower cost technology and has a relatively high enzyme production capacity (Camassola and Dillon, 2007;Camassola and Dillon, 2016;Macedo et al, 2013;Pirota et al, 2013;Yoon et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Fodder radish cake (Raphanus sativus L.) as an alternative biomass for the production of cellulases and xylanases in solid-state cultivation

Zukovski

Fontana

Pauletti

et al. 2017

Braz. J. Chem. Eng.

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Fodder radish (FR) is an oilseed crop with a high potential for biodiesel production due to its high productivity and the quality of its seed oil. FR oil extraction results in a residue that is rich in protein and fiber. In this study, FR cake (FRC) was evaluated as carbon and nitrogen source for the production of cellulases and xylanases using Penicillium echinulatum S1M29 during solid-state cultivation. It was determined that it is possible to partially replace wheat bran (WB) by FRC, resulting in 24.22 ± 0.25U/g Filter Paper Activity (144 hours), 210.5 ± 5.8U/g endoglucanase activity (144 hours), 22.62 ± 0.01U/g β-glucosidase activity (96 hours) and 784.7 ± 70.19U/g xylanase activity (120 hours). These values are equal or higher than the enzymatic activity obtained using WB. These results may contribute to the reduction of the cost of enzymes used in the production of cellulosic ethanol or other biotechnological applications.

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Enzymes for second generation ethanol: exploring new strategies for the use of xylose

Cited by 16 publications

References 39 publications

Use of elephant grass (Pennisetum purpureum) as substrate for cellulase and xylanase production in solid-state cultivation by Penicillium echinulatum

Use of elephant grass (Pennisetum purpureum) as substrate for cellulase and xylanase production in solid-state cultivation by Penicillium echinulatum

Is cellulase production by solid-state fermentation economically attractive for the second generation ethanol production?

Fodder radish cake (Raphanus sativus L.) as an alternative biomass for the production of cellulases and xylanases in solid-state cultivation

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