Enzymes Immobilization on Fe&lt;SUB&gt;3&lt;/SUB&gt;O&lt;SUB&gt;4&lt;/SUB&gt;-Silver Nanoparticles

Ferrite nanoparticles with nominal composition Me0.5Fe2.5O4 (Me = Co, Fe, Ni or Mn) have been successfully prepared by the wet chemical method. The obtained particles have a mean diameter of 11–16 ± 2 nm and were modified to improve their magnetic properties and chemical activity. The surface of the pristine nanoparticles was functionalized afterwards with –COOH and –NH2 groups to obtain a bioactive layer. To achieve our goal, two different modification approaches were realized. In the first one, glutaraldehyde was attached to the nanoparticles as a linker. In the second one, direct bonding of such nanoparticles with a bioparticle was studied. In subsequent steps, the nanoparticles were immobilized with enzymes such as albumin, glucose oxidase, lipase and trypsin as a test bioparticles. The characterization of the nanoparticles was acheived by transmission electron microscopy, X-ray diffraction, energy dispersive X-ray and Mössbauer spectroscopy. The effect of the obtained biocomposites was monitored by Fourier transform infrared spectroscopy. The obtained results show that in some cases the use of glutaraldehyde was crucial (albumin).

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“…The whole combination was mixed for 2 h at RT, then washed 3 times with PBS and dried at RT [16,36]. …”

Section: Methodsmentioning

confidence: 99%

Characterization of ferrite nanoparticles for preparation of biocomposites

Klekotka¹,

Rogowska²,

Satuła³

et al. 2017

Beilstein J. Nanotechnol.

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“…Ag shell was prepared by adding to magnetite seeds 2 or 1 mmol of AgNO 3 with 1,2-hexadecanediol, phenyl ether and oleyl amine, then mixed for 15 min at RT, next for 2 h at 30°C, and at the end for 30 min at 140°C. For other details of the synthesis procedure please see the other paper [14,26]. Nanoparticles with Au shell were prepared by the modified Robinson method [27].…”

Section: Preparation Of Magnetic and Nonmagnetic Inter Layermentioning

confidence: 99%

Magnetic nanoparticles of core–shell structure

Kalska-Szostko

Wykowska

Satuła

2015

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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“…Enzymes have long been used in industry as catalysts for catabolic processes or for the production of specific chemical enantiomers. MNP-enzyme conjugates (MNP-Es) represent a specific class of bio-MNP conjugates that are of great interest for biotechnological applications where high catalytic specificity, prolonged reaction time, and in some cases the ability to recycle an expensive biocatalyst is required 14,15,16 . In addition, magnetic field susceptibility provides a mechanism for efficient recovery of the enzyme complex from reaction products, which is especially important in the pharmaceutical industry where enzyme contamination of the final product can cause detrimental side effects.…”

Section: Introductionmentioning

confidence: 99%

Immobilization of the Magnetic Nanoparticles with Alkaline Protease Enzyme Produced by Enterococcus hirae and Pseudomonas aeruginosa Isolated from Dairy Effluents

Masi

Chandramohan

Ahmed

2018

Braz. arch. biol. technol.

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Protease is an enzyme which has a wide range of applications in various fields. Extracellular protease was produced from Pseudomonas aeruginosa and Enterococcus hirae which were isolated from the effluents of diary industries. Protease immobilized with super paramagnetic nanoparticles was characterized by DLS, XRD and TEM methods in relation to their size and structure. The protease enzyme was bound to magnetic nanoparticles via surface transformation technique including Silica coated magnetic Nano composite, amine and cysteine functioned Nano composite formation. Successful binding of protease onto the particles was confirmed by TEM imaging. The maximal enzyme activity of immobilized protease was determined using universal protease assay and was found to be 105µg mL -1 & 290µg mL -1 for Pseudomonas sp. and Enterococcus sp. respectively. The immobilization capacity of protease onto nanoparticles was 6000µM/g. The stability of the immobilized enzyme increased in comparison with the free enzyme. Overall, this study showed that the stability and activity of the protease was enhanced by immobilization to the magnetic nanoparticles. This suggested that immobilized enzyme on the magnetic beads of nanoparticles could be used in an interesting range of applications, both in broader temperature and pH ranges, also permitting magnetic recovery of the enzyme for reuse or purification of the product.

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Enzymes Immobilization on Fe<SUB>3</SUB>O<SUB>4</SUB>-Silver Nanoparticles

Cited by 6 publications

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Characterization of ferrite nanoparticles for preparation of biocomposites

Characterization of ferrite nanoparticles for preparation of biocomposites

Magnetic nanoparticles of core–shell structure

Immobilization of the Magnetic Nanoparticles with Alkaline Protease Enzyme Produced by Enterococcus hirae and Pseudomonas aeruginosa Isolated from Dairy Effluents

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