Enzymes in the Fruit Juice Industry

Lanzarini, G.; Pifferi, Pier Giorgio

doi:10.1007/978-94-009-1113-0_13

Cited by 27 publications

(19 citation statements)

References 43 publications

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“…Endo-␤-1,4-xylanases (A) and ␤-xylosidase (B) activities in culture supernatants of the QM9414 parental strain (gray diamonds) and the nx7 mutant strain (black squares) (A to C). Detection of enzyme activity formation of both strains was accomplished after 0, 12,24,36,48,60, and 72 h of cultivation in shake flasks on 1% (wt/vol) xylan after inoculation with spores. One unit of activity is defined as the amount of enzyme required to release 1 micromole of reducing xylose sugar equivalents or 4-nitrophenyl residues per minute under the defined assay conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Hydrolases secreted by this fungus are used in a broad range of industrial applications covering, e.g., pulp and paper (4,32,53), food and feed (10,24,52), and textile (20,23,33) industries as well as biofuels and bioenergy (14,17,36). The set of cellulolytic and xylanolytic enzymes produced by H. jecorina includes cellobiohydrolases (EC 3.2.1.91; reference 48, e.g.…”

mentioning

confidence: 99%

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Transcriptional Regulation of xyr1 , Encoding the Main Regulator of the Xylanolytic and Cellulolytic Enzyme System in Hypocrea jecorina

Mach-Aigner

Pucher

Steiger

et al. 2008

Appl Environ Microbiol

178

185

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In Hypocrea jecorina, Xyr1 (xylanase regulator 1) is the main transcription activator of hydrolase-encoding genes, such as xyn1, xyn2, bxl1, cbh1, cbh2, egl1, and bgl1. Even though Xyr1 mediates the induction signal for all these genes derived from various inducing carbon sources and compounds, xyr1 transcription itself is not inducible by any of these substances. However, cultivation on glucose as the carbon source provokes carbon catabolite repression of xyr1 transcription mediated by Cre1. In addition, xyr1 transcription is repressed by the specific transcription factor Ace1. Moreover, Xyr1 is permanently available in the cell, and no de novo synthesis of this factor is needed for a first induction of xyn1 transcription. The constitutive expression of xyr1 leads to a significant elevation/deregulation of the xyn1, xyn2, and bxl1 transcription compared to what is seen for the parental strain. Overall, the corresponding xylanolytic enzyme activities are clearly elevated in a constitutively xyr1-expressing strain, emphasizing this factor as an auspicious target for genetically engineered strain improvement.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Transcriptional Regulation of xyr1 , Encoding the Main Regulator of the Xylanolytic and Cellulolytic Enzyme System in Hypocrea jecorina

Mach-Aigner

Pucher

Steiger

et al. 2008

Appl Environ Microbiol

178

185

View full text Add to dashboard Cite

show abstract

“…Hydrolases secreted by this fungus are used in a broad range of industrial applications covering, for instance pulp and paper industry (Buchert et al, 1998;Welt and Dinus, 1995;Noé et al, 1986), food and feed industry (Galante et al, 1993;Walsh et al, 1993;Lanzarini and Pifferi, 1989), and textile industries (Koo et al, 1994;Kumar et al, 1994;Pedersen et al, 1992) as well as biofuels and bioenergy (Himmel et al, 2007;Hahn-Hägerdal et al, 2006;Ragauskas et al, 2006).…”

Section: Introductionmentioning

confidence: 97%

An accurate normalization strategy for RT-qPCR in Hypocrea jecorina (Trichoderma reesei)

Steiger

Mach

Mach-Aigner

2010

Journal of Biotechnology

103

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“…The enzymatic clarification was influenced by a number of variables including concentration of the enzyme, temperature and incubation time of the treatment (Neubeck, 1975;Baumann, 1981;Lanzarini & Pifferi, 1989). The present work involves optimization of different parameters affecting the depectinization rate.…”

Section: Introductionmentioning

confidence: 99%

Optimizing conditions for enzymatic clarification of banana juice using response surface methodology (RSM)

Lee

Yusof

Hamid

et al. 2006

Journal of Food Engineering

254

172

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Raw banana juice is turbid, viscous and gray in colour. This work was initiated to optimize the enzymatic clarification process of banana juice using response surface methodology. Banana juice was treated with pectinase at various enzyme concentrations (0.01-0.1%), temperatures (30-50°C) and time (30-120 min) of treatment. The effect of these enzyme treatments on filterability, clarity, turbidity and viscosity of the juice were studied by employing a second order central composite design. The coefficient of determination, R 2 values for filterability, clarity, turbidity and viscosity were greater than 0.900. Statistical analysis showed that filterability, clarity, viscosity and turbidity were significantly (p < 0.05) correlated to enzyme concentration, incubation temperature and incubation time. Enzyme concentration was the most important factor affecting the characteristics of the banana juice as it exerted a highly significant influence (p < 0.01) on all the dependent variables. An increase in time and/or concentration of enzyme treatment was associated with an increase in filterability and clarity, and decrease in turbidity and viscosity. Based on response surface and contour plots, the optimum conditions for clarifying the banana juice were: 0.084% enzyme concentration, incubation temperature of 43.2°C and incubation time of 80 min.

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Enzymes in the Fruit Juice Industry

Cited by 27 publications

References 43 publications

Transcriptional Regulation of xyr1 , Encoding the Main Regulator of the Xylanolytic and Cellulolytic Enzyme System in Hypocrea jecorina

Transcriptional Regulation of xyr1 , Encoding the Main Regulator of the Xylanolytic and Cellulolytic Enzyme System in Hypocrea jecorina

An accurate normalization strategy for RT-qPCR in Hypocrea jecorina (Trichoderma reesei)

Optimizing conditions for enzymatic clarification of banana juice using response surface methodology (RSM)

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