2015
DOI: 10.1007/s00203-015-1142-z
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Enzymes of anaerobic ethylbenzene and p-ethylphenol catabolism in ‘Aromatoleum aromaticum’: differentiation and differential induction

Abstract: The denitrifying bacterium 'Aromatoleum aromaticum' strain EbN1 is one of the best characterized bacteria regarding anaerobic ethylbenzene degradation. EbN1 also degrades various other aromatic and phenolic compounds in the absence of oxygen, one of them being p-ethylphenol. Despite having similar chemical structures, ethylbenzene and p-ethylphenol have been proposed to be metabolized by completely separate pathways. In this study, we established and applied biochemical and molecular biological methods to show… Show more

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Cited by 20 publications
(18 citation statements)
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References 45 publications
(121 reference statements)
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“…Compared with other alkyl aromatics biooxidation pathways in nature (5,48), such as xylene monooxygenase (49-51) and p-cresol methylhydroxylase (52), this unique chemomimetic system features a greater generality because the key P450 enzyme CreJ adopts the phosphate anchoring strategy for substrate recognition and binding. Protein BLAST searches reveal a large number of CreJ analogs (>40% sequence identity) from different genera of microorganisms.…”
Section: Discussionmentioning
confidence: 99%
“…Compared with other alkyl aromatics biooxidation pathways in nature (5,48), such as xylene monooxygenase (49-51) and p-cresol methylhydroxylase (52), this unique chemomimetic system features a greater generality because the key P450 enzyme CreJ adopts the phosphate anchoring strategy for substrate recognition and binding. Protein BLAST searches reveal a large number of CreJ analogs (>40% sequence identity) from different genera of microorganisms.…”
Section: Discussionmentioning
confidence: 99%
“…1). An accordingly substrate-specific regulation was demonstrated on the basis of targeted transcript analysis as well as differential proteomic and enzymatic profiling (16,20,21). Furthermore, generation of an unmarked ⌬etpR in-frame deletion mutation resulted in loss of anaerobic growth with p-ethylphenol and p-hydroxyacetophenone as well as respective transcript and protein formation, underpinning the essential role of EtpR (22).…”
mentioning
confidence: 99%
“… Scheme of genes and enzymes involved in anaerobic degradation of ethylbenzene and acetophenone in Aromatoleum aromaticum . Modified from Rabus et al (2002) , Kühner et al (2005) , Muhr et al (2015) . (A) Predicted functions of gene products are indicated by different colors: blue: acetophenone and ethylbenzene degradation, red: ethylbenzene degradation, black: regulation, gray: transposase/transposase fragment, white: proteins of unknown function.…”
Section: Introductionmentioning
confidence: 99%
“…The reconstituted enzyme exhibits a stoichiometry of two ATP hydrolyzed to ADP per acetophenone carboxylated ( Jobst et al, 2010 ). The generated benzoylacetate is subsequently activated to the CoA-thioester by a CoA-ligase ( Rabus and Heider, 1998 ; Rabus et al, 2002 ; Muhr et al, 2015 ), and benzoylacetyl-CoA is cleaved by a so far unknown thiolase to acetyl-CoA and benzoyl-CoA, the common intermediate in the anaerobic metabolism of aromatic compounds ( Heider and Fuchs, 1997a , b ; Carmona et al, 2009 ; Fuchs et al, 2011 ). The apc-bal operon is part of a larger gene cluster including the ebd-ped operon and genes coding for two two-component regulatory systems ( Figure 1 ; Rabus et al, 2002 ).…”
Section: Introductionmentioning
confidence: 99%
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