Enzymes of the human cervix uteri. Comparison of dehydrogenases of lactate, isocitrate, and phosphogluconate in malignant and non-malignant tissue samples.

Ayre, Heather A; Goldberg, David M.

doi:10.1038/bjc.1966.85

Cited by 25 publications

(9 citation statements)

References 23 publications

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“…Of the enzymes studied in this work, ICD would seem to be that which is most sub ject to change after radiation and would be worthy of further investi gation in more promising clinical situations. In contrast with LDH, we have found significantly higher levels of this enzyme in cervical carcinoma than in normal cervical epithelium (Ayre and Goldberg 1966).…”

Section: Discussioncontrasting

confidence: 83%

Serum Enzymes after Therapeutic Radiation

Watts¹,

Goldberg²

1967

Enzymol Biol Clin

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The activity of 11 serum enzymes was studied in patients with carcinoma of the cervix uteri undergoing deep x-ray radiotherapy. These were aldolase, alkaline phosphatase, isocitrate, lactate and malate dehydrogenases, glutamate oxalacetate and glutamate pyruvate transaminases, and four nucleases. While there were no recognizable patterns of enzyme response in the group as a whole, some patients showed pronounced increases in activity of one or more of the enzymes during some stage of radiotherapy. Isocitrate dehydrogenase appeared to be the enzyme most subject to change after radiation.

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Section: Discussioncontrasting

confidence: 83%

Serum Enzymes after Therapeutic Radiation

Watts¹,

Goldberg²

1967

Enzymol Biol Clin

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“…There is qualitative agreement between our results and those of Pedersen (1975) as presented in Table IV. Quantitative discrepancies Ayre and Goldberg (1966) and Kikuchi et al (1972) for the enzymes LDH and 6PGD, and HK, respectively. oxGPD was not studied by these workers; that this enzyme alone showed no significant increase in malignant tissue may indicate lack of hydrogen-transport capacity relative to glycolytic flux, necessitating formation of lactate for regeneration of NAD +.…”

Section: Discussionmentioning

confidence: 99%

“…The two-fold increases in activity of the pentose-phosphate-shunt dehydrogenases G6PD and 6PGD in malignant cervix were less than those quoted by other workers (Table IV). Ayre and Goldberg (1966) stressed the importance of the pentose-phosphate pathway to the malignant cell in providing ribose-5-phosphate and NADPH for nucleic-acid synthesis.…”

Section: Discussionmentioning

confidence: 99%

Enzymes of glucose metabolism in carcinoma of the cervix and endometrium of the human uterus

Marshall¹,

Goldberg²,

Neal³

et al. 1978

Br J Cancer

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Summary.-Twelve enzymes related to the direct oxidative and glycolytic pathways of glucose metabolism were assayed in 88 cancers of the cervix and 48 cancers of the endometrium of the human uterus, and the activities compared with those obtained from a group of control tissues. Significant increases for all but one of the enzymes studied (a-glycerolphosphate dehydrogenase) were found in cancer of the cervix, when compared with normal cervix epithelium. Hexokinase, phosphofructokinase, and aldolase appear to be rate-limiting in normal cervix epithelium; however, since the increase in activity of the first two in cancers was least of all the glycolytic enzymes, redundant enzyme synthesis probably occurs in the malignant cell for the enzymes catalysing reversible reactions. There was virtually no correlation between the activity of any enzyme measured in the cancer sample and histological assessments of the degree of malignancy of the tumour, or the clinical stage of the disease. All enzymes except pyruvate kinase had significantly higher activity in normal endometrium than in normal cervix epithelium, presumably reflecting the greater metabolic requirements of the former tissue. Only phosphoglucose isomerase and pyruvate kinase were significantly higher in endometrial cancer than in normal endometrium, and there were few significant differences between cancers of the cervix and of the endometrium, despite the marked differences in their tissues of origin. These results suggest the changes occur during malignant transformation to the activities of both regulatory enzymes and those catalysing reversible reactions, in a manner justifying the conclusion that the general metabolism of tumours is convergent.

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“…There have been several reports (Laurova et af, 1974;Pedersen, 1977;Marshall et af, 1978) in which a significant increase in total lactate dehydrogenase activity was found when malignant and normal tissues were compared. In contrast, Ayre and Goldberg (1966) found no such difference and Mainigi (1972) found an increase only in the case of chronic cervicitis. With carcinoma in situ, Latner et a1 (1966) occasionally detected an increase in lactate dehydrogenase activity and levels of this enzyme seem to be unequivocally raised in both hyperplasia and carcinoma of the endometrium (Fottrell et af, 1974;Laurova et af, 1974).…”

Section: Discwssionmentioning

confidence: 74%

The Effect of Carcinoma of the Cervix on the L‐lactate‐nicotinamide‐adeninedinucleotide Oxidoreductase Activity in Vaginal Aspirates

Emes

Latner

Guthrie

et al. 1981

BJOG

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Summary Lactate dehydrogenase (L‐lactate‐NAD+ oxidoreductase; EC 1. 1. 1. 27) has been assayed in saline extracts of vaginal aspirates obtained from patients with carcinoma in situ and from patients with clinically detectable invasive carcinoma of the cervix. Taking premenopausal and postmenopausal patients separately, significant differences were found between the aspirate lactate dehydrogenase activities obtained from patients with carcinoma in situ and the activities obtained from patients with invasive carcinoma; generally higher activities were obtained from the latter. With the exception of postmenopausal patients with carcinoma in situ, aspirate enzyme activities obtained from both groups of patients were significantly different from those obtained from normal women. Significantly higher lactate dehydrogenase activity was found in aspirates from patients for whom histology showed the presence of an adenocarcinoma or certain highly malignant cell types. Treatment by radium insertion usually resulted in a fall in total lactate dehydrogenase activity. Significant differences between patient groups were found for the proportions of single lactate dehydrogenase isoenzymes in a few instances but no significant difference in overall isoenzyme distribution was found.

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Enzymes of the human cervix uteri. Comparison of dehydrogenases of lactate, isocitrate, and phosphogluconate in malignant and non-malignant tissue samples.

Cited by 25 publications

References 23 publications

Serum Enzymes after Therapeutic Radiation

Serum Enzymes after Therapeutic Radiation

Enzymes of glucose metabolism in carcinoma of the cervix and endometrium of the human uterus

The Effect of Carcinoma of the Cervix on the L‐lactate‐nicotinamide‐adeninedinucleotide Oxidoreductase Activity in Vaginal Aspirates

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