Enzymes of Tryptophan Biosynthesis in
            <i>Serratia marcescens</i>

Hutchinson, Mary Anne; Belser, William L.

doi:10.1128/jb.98.1.109-115.1969

Cited by 38 publications

(18 citation statements)

References 24 publications

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“…There were five distinct patterns found upon elution of the column (Fig. 2); in addition to the two patterns described previously (19,20) for the enteric bacteria, three new patterns were found.…”

Section: Resultssupporting

confidence: 54%

“…The type 5 pattern has been described previously (19). This pattern differs from type 4 only in that the PRT activity has a lower molecular weight and co-elutes with PRAI and InGPs at a molecular weight of 45,000.…”

Section: Resultsmentioning

confidence: 70%

“…Liquid cultures of all orga- ferred to as PED buffer). The procedures employed were described previously (19).…”

mentioning

confidence: 99%

“…Enzyme assays. All assays were performed as described previously (19,37). Unless otherwise indicated in the text, a unit of activity is defined as 1 nmol of substrate disappearing or 1 nmol of product appearing per min (m.i.e.u.…”

mentioning

confidence: 99%

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Tryptophan biosynthetic pathway in the Enterobacteriaceae: some physical properties of the enzymes

Largen¹,

Belser²

1975

J Bacteriol

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Several physical properties of the first four enzymatic activities of the tryptophan pathway were examined using gel filtration and ion exchange chromatography. Five different patterns were noted. Differences in the anthranilate synthetase (AS) and phosphoribosylanthranilate transferase (PRT) defined these patterns. In all the organisms studied phosphoribosylanthranilate isomerase and indoleglycerol phosphate synthetase co-eluted from both diethylaminoethyl-cellulose and G-200 and thus probably are contained in a single polypeptide of 50,000 daltons. An AS-PRT complex was found in Citrobacter species, Enterobacter cloacae, and Erwinia dissolvens. In all the other bacteria examined AS and PRT were separate molecules. In Serratia marcescens, S. marinorubra, and Enterobacter liquefaciens, AS was 140,000 daltons and PRT was 45,000 daltons. In Erwinia carotavora and Enterobacter hafniae the AS was the same size as the Serratia species but the PRT was larger at 67,000 daltons. Two Proteus species had an AS and PRT of the same size as E. carotavora and E. hafniae but the Proteus AS was different in that it partially dissociated upon gel filtration. Aeramonas formicans was unique in its possession of an AS with a molecular weight of 220,000. The PRT of A. formicans was found to elute at 67,000 daltons. Possible paths of evolution of the tryptophan enzymes are discussed in terms of the reults of this study. The results presented here are also considered with respect to existing taxonomic schemes of the enteric bacteria. ucts of these genes, five different distribution patterns were found. These results were considered with respect to existing phylogenetic schemes and were used to clarify some of the natural relationships among the fungi. 239 on July 31, 2020 by guest

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Section: Resultssupporting

confidence: 54%

Section: Resultsmentioning

confidence: 70%

“…Liquid cultures of all orga- ferred to as PED buffer). The procedures employed were described previously (19).…”

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Tryptophan biosynthetic pathway in the Enterobacteriaceae: some physical properties of the enzymes

Largen¹,

Belser²

1975

J Bacteriol

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show abstract

“…Based upon their enzymological analysis, Hutchinson and Belser [12] suggested that the genes concerned with tryptophan biosynthesis in S. marcescens were clustered, and supposedly constitute an operon.…”

Section: Discussionmentioning

confidence: 99%