Enzymic and Genetic Control of Polyphosphate Accumulation in Aerobacter aerogenes

Harold, Franklin M.

doi:10.1099/00221287-35-1-81

Cited by 43 publications

(35 citation statements)

References 13 publications

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“…However, this does not appear to be utilized as an energy source (Harold 1966). Shaposhnikov and Fedorov (1960) reported that dark incubation of Chlorobium induced a slight degradation of polyphosphate, but the amount of polyphosphate mobilized was only about one-tenth of the total pool.…”

Section: Discussionmentioning

confidence: 96%

“…The occurrence of polyphosphate bodies in cyanobacteria is well documented (Jensen 1968(Jensen , 1969Jensen and Sicko 1974). Jensen and Sicko (1974) are exposed to phosphate concentrations which are greater than required (Harold 1964). This storage activity is enhanced when cells are first starved of phosphate and then placed in a condition of high phosphate (Shapiro 1967).…”

Section: Discussionmentioning

confidence: 98%

“…Early reports suggested the possibility of the polyphosphate serving as a phosphagen, a reserve energy source. However, several studies have shown this to be unlikely, and the major feature in the metabolism of polyphosphates may be the regulation of Pi and Ht concentrations (Harold 1966).…”

Section: Introductionmentioning

confidence: 99%

“…This accumulation, referred to as luxury consumption (Shapiro 1968) or phosphate overplus (Liss and Langen 1962;Harold 1964), occurs in a wide variety of organisms (Harold 1966).…”

Section: Introductionmentioning

confidence: 99%

“…Polyphosphate kinase, polyphosphate adenosine monophosphate phosphotransferase, polyphosphate glucokinase, and various other enzymes have been implicated in the degradation of polyphosphate bodies (Harold 1966). Acid phosphatase (EC 3.1.3.2), also known as orthophosphoric monoester phosphorylase, is the principal enzyme responsible for the hydrolysis of polyphosphate bodies, the product being orthophosphate (Bowen and Bryant 1978).…”

Section: Introductionmentioning

confidence: 99%

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Polyphosphate body and acid phosphatase localization in Nostoc sp.

DuBois¹,

Roberts²,

Kapustka³

1984

Can. J. Microbiol.

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Polyphosphate body and acid phosphatase localization in Nostocsp. Can. J. . Polyphosphate bodies and acid phosphatase activity were characterized in Nostoc sp. to determine if the hydrolysis of polyphosphate bodies occurs during dark (energy stress) periods. Electron and light microscopy were used to locate polyphosphate bodies. Acid phosphatase activity was measured using p-nitrophenyl phosphate as the substrate to determine net changes in the level of the enzyme activity. To induce energy stress, Nostoc sp. cells were kept in the dark for 72 h to deplete stored carbon compounds. Cells incubated in the light for 72 h (controls) showed acid phosphatase activity localized around the perimeter of polyphosphate bodies. When cells were incubated in the dark. acid phosphatase activity occurred throughout the polyphosphate body matrix. However, complete hydrolysis of the polyphosphate body did not occur and the rate of acid phosphatase activity was not affected. DUBOIS, J. D . , K. R . ROBERTS et L. A. KAPUSTKA. 1984. Polyphosphate body and acid phosphatase localization in Nosroc sp. Can. J. Microbiol. 30: 8-15. Des corpuscules de polyphosphate et I'activitt phosphatase acide ont t t t caractkrists chez Nostoc sp. pour dtterminer si l'hydrolyse des corpuscules de polyphosphate a lieu au cours des periodes d'obscuritt (stress d'tnergie). Les corpuscules de polyphosphate ont t t t localists par microscopies photonique et Clectronique. L'activitt phosphatase acide a t t t mesurte a l'aide de p-nitrophtnyl phosphate comme substrat pour dtterminer les tchanges nets aux difftrents niveaux d'activitt de I'enzyme. Pour induire un stress d'tnergie, des cellules de Nostoc sp. ont t t t conservees en obscuritt durant 72 h en vue d'tpuiser les composCs carbonks de rtserve. Les cellules-ttmoin, incubtes en lumikre durant 72 h, ont prtsentt une activitt phosphataseacide localiste au niveau du ptrimktre des corpuscules de polyphosphate. Chez les cellules incubtes en obscuritt, I'activitt phosphatase acide s'est manifestte a travers la matrice entiere des corpuscules de polyphosphate. Toutefois, il n'y a pas eu hydrolyse complete des corpuscules de polyphosphate et le taux d'activitt phosphatasique acide n'a pas t t t affectt.[Traduit par le journal]

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

“…This accumulation, referred to as luxury consumption (Shapiro 1968) or phosphate overplus (Liss and Langen 1962;Harold 1964), occurs in a wide variety of organisms (Harold 1966).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Polyphosphate body and acid phosphatase localization in Nostoc sp.

DuBois¹,

Roberts²,

Kapustka³

1984

Can. J. Microbiol.

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Phosphorus uptake and growth of blue‐green alga, Microcystis aeruginosa

Okada

Sudo

Aiba

1982

Biotech & Bioengineering

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The specific uptake rate Q(p) of orthophosphate (expressed throughout as phosphorus) and the specific growth rate mu of Microcystis aeruginosa were measured using batch-precultured cells, whose growth phase, and intracellular and extracellular phosphorus concentrations f(p) and P, respectively, had been changed. When the cells from phosphorus-rich precultures were used, smaller values of Q(p) (0.1-0.3 microg P mg dry wt. (-1) h (-1)) were observed. However, if phosphorusstarved cells were used, the initial value of Q(p) was enhanced to more than ten times those smaller values referred to above, but declined rapidly with time after the transfer. Q(p) leveled off at around t = 4 h, when f(p) approached the maximum value, even if phosphorus was still available in the medium. A new correlation was presented here with respect to Q(p) as a function of P and f(p) as follows: [Qp = Qp,max P/Kp + P (fp,max-fp)/fp,max-fp)] Although numerical values of parameters involved in the equation depend on physiological state (or preculture history) of the cells, the above equation could account not only for phosphorus uptake, during which changes in phosphorus content in the cells were observed, but also for initial rates of uptake presented previously by other workers. mu Values were confirmed to be a hyperbolic function of f(p) as has been suggested by previous workers.

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Kinetics of growth and phosphate uptake in pure culture studies of Acinetobacter species

Hao

Chang

1987

Biotech & Bioengineering

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Acinetobacter has been found to be the major species responsible for mediating biological phosphate removal. The growth kinetics and phosphate uptake were investigated for an isolated Acinetobacter strain growing in a defined medium. The phosphate uptake is dependent on growth rate, temperature, and pH. Polyphosphate granules occurred in a balanced growth stage. The maximum phosphorus content in cells was 4.8% at the dilution rate of 12 day(-1). The specific phosphate uptake rate was found to be a quadratic polynomial function of the dilution rate. Increased calcium (up to 36 mg/L) and magnesium (up to 15 mg/L), and the addition of yeast extract (100 mg/L), primary effluent (20%), and fluoride (10 mg/L) did not affect phosphate uptake. Anaerobic conditioning (N(2) stripping), low pH (CO(2) stripping), and addition of sodium acetate under anaerobic conditions failed to stimulate immediate phosphate release. Nevertheless, After 21-24 h, the phosphate release was ca. 3, 5, and 15 mg P/g cell, respectively, for N(2) purging, the addition of acetate, and CO(2) purging. For two-stage completely stirred reactor operation, there was negligible phosphate overplus at the second reactor when phosphate was added, when the first reactor was subjected to phosphate limitation. When both phosphate and carbon limited the growth in the first reactor, there was slight phosphate accumulation under endogenous respiration conditions in the second reactor.

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Enzymic and Genetic Control of Polyphosphate Accumulation in Aerobacter aerogenes

Cited by 43 publications

References 13 publications

Polyphosphate body and acid phosphatase localization in Nostoc sp.

Polyphosphate body and acid phosphatase localization in Nostoc sp.

Phosphorus uptake and growth of blue‐green alga, Microcystis aeruginosa

Kinetics of growth and phosphate uptake in pure culture studies of Acinetobacter species

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