Enzymic and <i>in sacco</i> methods to estimate rumen degradation of food protein in cattle

Chaudhry, AS

doi:10.1002/jsfa.3021

Cited by 11 publications

(15 citation statements)

References 21 publications

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“…The patterns of mean degradation with increasing time from 0 to 96 h for the fresh inocula also compared well with the published values of similar feeds that were tested in sacco in fistulated animals (AFRC, 1993;Chaudhry, 2007;Mohamed and Chaudhry, 2008). In fact the degradation of grass nuts in this study was similar to the earlier data for a different sample of the same batch of grass nuts (Chaudhry, 2008).…”

Section: Discussionsupporting

confidence: 87%

“…The residues were also analysed for nitrogen to estimate CP (N × 6.25) degradation (CPD). Triplicate samples of each pre-incubated feed were also analysed for DM, OM, CP, EE, NDF, and ADF by using appropriate methods as previously described by Chaudhry (2007).…”

Section: Incubation Of Feeds and Chemical Analysismentioning

confidence: 99%

“…The rumen degradation data for different feeds are traditionally obtained by using surgically modified animals for either in sacco studies or to obtain rumen fluid for in vitro studies (Chaudhry, 2007(Chaudhry, , 2008; Mohamed and Chaudhry, 2008;Chaudhry and Mohamed, 2011). Due to high management and welfare costs of maintaining rumen fistulated animals, the availability of such rumen fluid to commercial laboratories on regular basis is restricted.…”

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confidence: 99%

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Fresh or frozen rumen contents from slaughtered cattle to estimatein vitro degradation of two contrasting feeds

Chaudhry¹,

Mohamed²

2012

CZECH J ANIM SCI

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ABSTRACT:The present factorial experiment tested the thawed rumen fluid from frozen rumen contents against fresh rumen fluid from the same slaughtered cattle to estimate in vitro degradation of rapeseed meal (RSM) and grass nuts (GN) as two contrasting feeds at various times. Fresh rumen fluid showed higher (P < 0.05) in vitro dry matter (DMD) and crude protein (CPD) degradation than the thawed rumen fluid at most incubation times. Significant differences (P < 0.05) were found between fresh and differently prepared thawed rumen fluids for mean degradation constants (a, b, c) and effective degradability (P 0.02 ) of feeds. While the thawed rumen fluids had lower (P < 0.01) degradation than the fresh rumen fluid, their degradation values correlated very well (R 2 ≥ 0.95) with those for the fresh rumen fluid. It appeared that the thawed rumen fluid can be used to predict in vitro degradation of the selected feeds when frequent access to slaughtered or fistulated animals is restricted. Nevertheless it would be vital to improve the activity of thawed rumen fluid prior to testing its suitability for numerous feeds before recommending its routine use to estimate degradation of ruminant feeds.

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Section: Discussionsupporting

confidence: 87%

Section: Incubation Of Feeds and Chemical Analysismentioning

confidence: 99%

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confidence: 99%

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Fresh or frozen rumen contents from slaughtered cattle to estimatein vitro degradation of two contrasting feeds

Chaudhry¹,

Mohamed²

2012

CZECH J ANIM SCI

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“…Some of these methods have used solvents [1,2] or enzymes [3][4][5][6] instead of rumen fluid to study kinetics of rumen degradation. However, the validity of these methods depended upon the availability of either in sacco or rumen fluid based in vitro data for making comparisons [7].…”

Section: Introductionmentioning

confidence: 99%

Slaughtered Cattle as a Source of Rumen Fluid to Evaluate Supplements for In Vitro Degradation of Grass Nuts and Barley Straw

Chaudhry¹

2008

TOVSJ

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This factorial study examined the potential of slaughtered cattle as a source of rumen fluid (RF) to test the impact of 0, 90, 180 g/kg forage (Amount) of 2 supplements (Low protein of 12% =LP; high protein of 25% =HP) on in vitro rumen degradation of grass nuts (Grass) and barley straw (Straw) over various times. RF was collected from three slaughtered cattle (Animal) on three separate occasions and buffered under CO 2 as inoculum at 39 o C. About 1g ground Straw or Grass and each amount of LP or HP were mixed in separate test tubes with 50 ml inoculum under CO 2 . The tubes fitted with Bunsen valves were incubated at 0-72h after which the contents were washed and dried to estimate dry matter disappearance (DMD). The DMD were statistically analysed for the main effects of Animal, Forage, Supplement, Amount and their interactions at each incubation but not Animal based interactions. The Forage, Amount and Animal effects were significant (P<0.01) at most incubations whereas Supplement effect was significant at 72h only showing greater DMD for HP than LP (P<0.05). DMD increased with time for all main effects. Grass had greater DMD than Straw at all incubations (P<0.001). DMD increased (P<0.05) with time but not at 0h with increasing Amount and increased DMD was greater from 0 to 90 g/kg than at 90 to 180 g/kg. The animals differed (P<0.05) at all but 0h showing variable responses with the confounding effect of their gender, age, breed, size and pre-slaughter feeding. Apparently slaughtered cattle can be used to obtain RF to evaluate supplements for in vitro forage degradation. However, the selection of suitable donor animals of similar pre-slaughter characteristics would be critical if in vitro studies involving slaughtered animals are to be used to evaluate feed supplements before their use for grazing ruminants.

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“…Proteases with different origins have been tested by some researchers to determine ruminal protein degradability. The most commonly used protease enzyme was the enzyme which has been extracted from Streptomyces griseus (Krishnamoorthy et al, 1983;Chaudhry, 2005Chaudhry, , 2007. Aufrere et al (1991) reported that in the French protein system (digestible proteins in the intestine, PDI), the enzymatic method is used as a laboratory method for nitrogen evaluation.…”

Section: Introductionmentioning

confidence: 99%

Estimation of Relationship Between In Situ and In Vitro Rumen Protein Degradability of Extruded Full Fat Soybean

Tunç

Çufadar

Yaman

2017

Turkish JAF Sci.Tech.

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The objectives of this study were to estimate the protein degradability of extruded full fat soybean (ESB) by in situ (nylon bag) and in vitro enzymatic method and to develop an equation in order predict in situ degradability from in vitro values. In the study enzymatic technique; hydrolysis after 1 h (INV 1 ) and after 24 h (INV 24 ) by a purified protease extracted from Streptomyces griseus in a borate-phosphate buffer at pH 8 was used as in vitro method. Relationship between in situ effective protein degradability (INS E ) and in vitro degradability after 1 and 24 hours incubations (INV 1 and INV 24 ) were determined. In situ protein degradability was measured at 0, 2, 4, 8, 16, 24, and 48 and at 72 h incubations in the rumen of 3 Holstein cows. In the study INS E , INV 1 and INV 24 were determined as 58.05, 20.24 and 41.46% respectively. Despite there were differences between in situ and in vitro protein degradability values, correlation coefficients between in situ and in vitro protein degradability of ESB were high and regression equations for estimation of in situ from in vitro were found significant. As conclusion in vitro enzymatic protein degradability (INV 1 and INV 24 ) can be used for estimation of in situ effective protein degradability of extruded full fat soybean.

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Enzymic and in sacco methods to estimate rumen degradation of food protein in cattle

Abstract: Despite variations between in sacco and enzyme estimates for different foods, the relationships between these estimates suggest that the HLA enzyme method has the potential to estimate food degradation. Copyright © 2007 Society of Chemical Industry.

Cited by 11 publications

References 21 publications

Fresh or frozen rumen contents from slaughtered cattle to estimatein vitro degradation of two contrasting feeds

Fresh or frozen rumen contents from slaughtered cattle to estimatein vitro degradation of two contrasting feeds

Slaughtered Cattle as a Source of Rumen Fluid to Evaluate Supplements for In Vitro Degradation of Grass Nuts and Barley Straw

Estimation of Relationship Between In Situ and In Vitro Rumen Protein Degradability of Extruded Full Fat Soybean

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