1969
DOI: 10.1021/bi00840a037
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Enzymic synthesis of deoxyribonucleic acid. XXXIV. Termination of chain growth by a 2',3'-dideoxyribonucleotide

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Cited by 156 publications
(74 citation statements)
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“…A wide variety of nucleoside 5'-triphosphates modified at the sugar moiety was tested as chain terminators for a number of polynucleotide synthesizing enzymes [1][2][3][4][5][6][7][8][9][10][11][12][13][14]. Among these we have found dNTP(3'-NH2) which is equally active against most of the tested DNA polymerases [7][8][9][10][11][12], araNTP(3'-NH2) which is active against all but E. coli DNA polymerase I [9,10], dNTP(3'-Nfluoroscaminyl) which is not inhibitory only for the terminal deoxyribonucleotidyl transferase [10], and dNTP(3'-N3) and araNTP(3'-N3) active against AMV reverse transcriptase [10,12].…”
Section: Introduction 2 Materials and Methodsmentioning
confidence: 99%
“…A wide variety of nucleoside 5'-triphosphates modified at the sugar moiety was tested as chain terminators for a number of polynucleotide synthesizing enzymes [1][2][3][4][5][6][7][8][9][10][11][12][13][14]. Among these we have found dNTP(3'-NH2) which is equally active against most of the tested DNA polymerases [7][8][9][10][11][12], araNTP(3'-NH2) which is active against all but E. coli DNA polymerase I [9,10], dNTP(3'-Nfluoroscaminyl) which is not inhibitory only for the terminal deoxyribonucleotidyl transferase [10], and dNTP(3'-N3) and araNTP(3'-N3) active against AMV reverse transcriptase [10,12].…”
Section: Introduction 2 Materials and Methodsmentioning
confidence: 99%
“…Increasing concentrations of ddTTP reduce the deoxyribonucleoside triphosphato incorporation into E . coli DNA more and more ( [9]. The enzyme was preincubated with poly-[d(A-T)], dATP and ddTTP for 30 min, and then the incorporation of [3H]dTTP was measured.…”
Section: Resultsmentioning
confidence: 99%
“…This nucleotide analogue terminates DNA synthesis by E . coli DNA polymerase I as it blocks the 3'-end of a growing DNA chain [9]. The covalent linkage to the 3'-OH end of a DNA chain was also demonstrated for 2',3'-dideoxyadenosine triphosphate [lo].…”
mentioning
confidence: 99%
“…For this reason, the elongated template was blocked on the 3'-terminus with 2',3'-dideoxyadenylate which was shown by Atkinson et al (12) to inhibit this 3'-nuclease activity.…”
Section: A-t-t-a-a) and D(pa-a-g-a-c-a-g-c-a-t-a-t)(l) The Preparatimentioning
confidence: 99%
“…Thus, termination with arabinosyl nucleotides (12) or even termination with a 3-phosphate (14) could be expected to inhibit or prevent the 3'-exonuclease action.…”
Section: -V(b)mentioning
confidence: 99%