Eosinophil cationic protein and histamine production by neutrophils from patients with periodontitis

Ribas, David; Fernández‐Carranco, M. Carmen; Hajji, Nabil; Bobadilla, P; Monteseirı́n, Javier

doi:10.1902/jop.2017.160679

Cited by 15 publications

(7 citation statements)

References 45 publications

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“…We also found a positive correlation between ECP and hs-CRP level and neutrophil count. The latter correlation is in accordance with the ability of neutrophils to synthetise and release ECP on activation 50 51. Overall, the associations between ECP, BMI, neutrophil count, hs-CRP and chronic bronchitis could be related to a distinct asthma phenotype.…”

Section: Discussionsupporting

confidence: 75%

Blood eosinophil cationic protein and eosinophil-derived neurotoxin are associated with different asthma expression and evolution in adults

Granger

Zerimech

Arab

et al. 2021

Thorax

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BackgroundEosinophil-derived neurotoxin (EDN) and eosinophil cationic protein (ECP) are proteins released by activated eosinophils whose role in adult asthma remains unclear.ObjectiveTo study associations between ECP, EDN and various asthma characteristics in adults from the Epidemiological Study on the Genetics and Environment of Asthma (EGEA).MethodsPlasma ECP and EDN levels were measured by ELISA. Cross-sectional analyses were performed in 941 adults (43±16 years old, 39% with asthma) at EGEA2 (2003–2007). Longitudinal analyses investigated the associations between EDN level at EGEA2 and changes in asthma characteristics between EGEA2 and EGEA3 (2011–2013, n=817). We used generalised estimated equations adjusted for age, sex, smoking status and body mass index to take into account familial dependence.ResultsAt EGEA2, both high ECP and EDN levels were associated with current asthma (adjusted OR (aOR) (95% CI): 1.69 (1.35–2.12) and 2.12 (1.76–2.57)). Among asthmatics, high EDN level was associated with asthma attacks (aOR: 1.50 (1.13–1.99)), wheezing and breathlessness (aOR: 1.38 (1.05–1.80)), use of asthma treatments (aOR: 1.91 (1.37–2.68)) and bronchial hyper-responsiveness (aOR: 2.03 (1.38–2.97)), even after further adjustment on ECP. High ECP level was associated with high neutrophil count and tended to be associated with chronic bronchitis. High EDN level at EGEA2 was associated with persistent asthma (aOR: 1.62 (1.04–2.52)), nocturnal symptoms (aOR from 2.19 to 3.57), worsening wheezing and breathlessness (aOR: 1.97 (1.36–2.85)) and nocturnal shortness of breath (aOR: 1.44 (1.04–1.98)) between EGEA2 and EGEA3.ConclusionsEDN and ECP were associated with different asthma expression in adults. EDN could be a potential biomarker to monitor asthma evolution in adults.

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Section: Discussionsupporting

confidence: 75%

Blood eosinophil cationic protein and eosinophil-derived neurotoxin are associated with different asthma expression and evolution in adults

Granger

Zerimech

Arab

et al. 2021

Thorax

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“…The increase in eotaxin‐1 observed in our study correlates with increased eosinophil cationic protein (ECP) expression in PD patients 66 since it is chemotactic for eosinophils 67,68 . Similarly, eotaxin‐3 (CCL26) aids in the recruitment of both basophils and eosinophils 69 causing excessive production of cytotoxic ECPs 70 consequently resulting in degradation of the periodontium 71 . The elevation in macrophage inflammatory proteins (MIP‐1α and MIP‐1β) in IL‐1β stimulated hPDLFs correlates positively with increased expression of MIP‐1α in the saliva of PD patients 72 which are absent in gingival tissues of healthy individuals 73 .…”

Section: Discussionmentioning

confidence: 53%

Cannabinoid type‐2 receptor agonist, inverse agonist, and anandamide regulation of inflammatory responses in IL‐1β stimulated primary human periodontal ligament fibroblasts

Abidi

Alghamdi

Dabbous

et al. 2020

J of Periodontal Research

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Objective The aim of this study is to understand the role of cannabinoid type 2 receptor (CB2R) during periodontal inflammation and to identify anti‐inflammatory agents for the development of drugs to treat periodontitis (PD). Background Cannabinoid type 2 receptor is found in periodontal tissue at sites of inflammation/infection. Our previous study demonstrated anti‐inflammatory responses in human periodontal ligament fibroblasts (hPDLFs) via CB2R ligands. Methods Anandamide (AEA), HU‐308 (agonist), and SMM‐189 (inverse agonist) were tested for effects on IL‐1β‐stimulated cytokines, chemokines, and angiogenic and vascular markers expressed by hPDLFs using Mesoscale Discovery V‐Plex Kits. Signal transduction pathways (p‐c‐Jun, p‐ERK, p‐p‐38, p‐JNK, p‐CREB, and p‐NF‐kB) were investigated using Cisbio HTRF kits. ACTOne and Tango™ ‐BLA functional assays were used to measure cyclic AMP (cAMP) and β‐arrestin activity. Results IL‐1β stimulated hPDLF production of 18/39 analytes, which were downregulated by the CB2R agonist and the inverse agonist. AEA exhibited pro‐inflammatory and anti‐inflammatory effects. IL‐1β increased phosphoproteins within the first hour except p‐JNK. CB2R ligands attenuated p‐p38 and p‐NFĸB, but a late rise in p‐38 was seen with HU‐308. As p‐ERK levels declined, a significant increase in p‐ERK was observed later in the time course by synthetic CB2R ligands. P‐JNK was significantly affected by SMM‐189 only, while p‐CREB was elevated significantly by CB2R ligands at 180 minutes. HU‐308 affected both cAMP and β‐arrestin pathway. SMM‐189 only stimulated cAMP. Conclusion The findings that CB2R agonist and inverse agonist may potentially regulate inflammation suggest that development of CB2R therapeutics could improve on current treatments for PD and other oral inflammatory pathologies.

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“…All of these changes are influenced by cytokines [e.g., granulocyte-colony stimulating factor (G-CSF)] (25), miRNAs [e.g., nod-like receptor 12 (NLRP12)] (32) and inflammasomes [e.g., nod-like receptor 12 (NLRP12)] (33). Proinflammatory cytokines [e.g., tumor necrosis factor (TNF)-a and interleukin (IL)-8], neutrophil enzymes, eosinophil cationic protein (ECP), histidine decarboxylase, histamine and neutrophil elastase (NE) secreted by neutrophils are increased (34)(35)(36)(37) and anti-inflammatory cytokines such as IL-10 are decreased (38). All of these changes are affected by bacteria (such as P. gingivalis) and bacterial products [such as leukocyte toxin (LtxA)] (36,38).…”

Section: Drug Therapy Drugs Targeting Neutrophilsmentioning

confidence: 99%

Immunomodulation in the Treatment of Periodontitis: Progress and Perspectives

et al. 2021

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Periodontitis is one of the most common dental diseases. Compared with healthy periodontal tissues, the immune microenvironment plays the key role in periodontitis by allowing the invasion of pathogens. It is possible that modulating the immune microenvironment can supplement traditional treatments and may even promote periodontal regeneration by using stem cells, bacteria, etc. New anti-inflammatory therapies can enhance the generation of a viable local immune microenvironment and promote cell homing and tissue formation, thereby achieving higher levels of immune regulation and tissue repair. We screened recent studies to summarize the advances of the immunomodulatory treatments for periodontitis in the aspects of drug therapy, microbial therapy, stem cell therapy, gene therapy and other therapies. In addition, we included the changes of immune cells and cytokines in the immune microenvironment of periodontitis in the section of drug therapy so as to make it clearer how the treatments took effects accordingly. In the future, more research needs to be done to improve immunotherapy methods and understand the risks and long-term efficacy of these methods in periodontitis.

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Eosinophil cationic protein and histamine production by neutrophils from patients with periodontitis

Abstract: The findings described could represent new knowledge of neutrophils as source of histamine and ECP in the progression of the periodontitis disease.

Cited by 15 publications

References 45 publications

Blood eosinophil cationic protein and eosinophil-derived neurotoxin are associated with different asthma expression and evolution in adults

Blood eosinophil cationic protein and eosinophil-derived neurotoxin are associated with different asthma expression and evolution in adults

Cannabinoid type‐2 receptor agonist, inverse agonist, and anandamide regulation of inflammatory responses in IL‐1β stimulated primary human periodontal ligament fibroblasts

Immunomodulation in the Treatment of Periodontitis: Progress and Perspectives

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