Eosinophil membrane changes during interaction with antibody-coated non-phagocytosable surfaces

Thorne, Kareen J. I.; Oliver, Rhonda C.; Glauert, Audrey M.

doi:10.1042/bst0090071

Cited by 3 publications

(3 citation statements)

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“…As previously reported [39], the net decrease in target lysis with the polyanion heparin could reflect the inhibition of lytic cationic products released by the eosinophils in the medium. The induc tion of exocytosis process may be linked to changes in the organization of the eosinophil membrane proteins and to the rearrangement of surface receptors [40] in close interaction with the target. Previous studies of in vitro induction of eosinophil degranulation showed that lectins cause vacuolation and/or degranulation [30,31], According to the biochemical potentiality of select ed eosinophils, our data confirm the results of Bass et al [22,23].…”

Section: Discussionmentioning

confidence: 99%

Heterogeneity of Human Peripheral Blood Eosinophils: Variability in Cell Density and Cytotoxic Ability in Relation to the Level and the Origin of Hypereosinophilia

Prin

Capron

Tonnel

et al. 1983

Int Arch Allergy Immunol

174

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Considerable variations in the distribution of eosinophil populations were revealed by density gradient centrifugation of peripheral blood leukocytes from 14 normal subjects and 31 patients with a transient or persistent hypereosinophilia. Such a purification procedure enabled us to collect in healthy controls, eosinophils (77.1 ± 15.9% SD purity) with an appreciable cell recovery (21.7 ± 14.0% SD) in the densest gradients (‘normodense’ cells). A high degree of purity was obtained in the same gradients, with leukocytes from patients with hypereosinophilia (84.4 ± 11.9% SD pure eosinophils) but the cell recovery was significantly decreased (4.1 ± 3.4% SD; p < 0.001). A study of the various fractions found to contain eosinophils showed cells with an altered cellular density (‘hypodense’ cells) especially in marked hypereosinophilia. Furthermore, studies on leukocytes from patients with a very high hypereosinophilia (differential cell count > 70%) led to the recovery of almost pure fractions of eosinophils in the low density gradients. Functional studies were performed on each distinct cell fraction collected. They included investigations of their cytotoxic ability in a heterologous antibody-dependent cell-mediated cytotoxicity assay and their biochemical activity by using a previously described technique evaluating the membrane hexose transport. Variability in the functional potentialities was observed in relation to the cellular density: higher cytotoxic eosinophil abilities were noted in the case of the ‘hypodense’ cell population. According to biochemical criteria, these latter cells appear to be more activated and capable of responding to stimulation.

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Section: Discussionmentioning

confidence: 99%

Heterogeneity of Human Peripheral Blood Eosinophils: Variability in Cell Density and Cytotoxic Ability in Relation to the Level and the Origin of Hypereosinophilia

Prin

Capron

Tonnel

et al. 1983

Int Arch Allergy Immunol

174

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“…Our results are in general agreement with those of Willinger et al (32,33) who studied polymorphonuclear leukocyte phagocytosis, and Thorne et al . who examined eosinophil adherence to an antibody-coated surface (28) and polymorphonuclear leukocyte phagocytosis (27) . Following eosinophil adherence to an antibody-coated surface, newly accessible proteins of 55,000 and 58,000 daltons appear in one-dimensional gels.…”

Section: Discussionmentioning

confidence: 99%

“…Many workers have analyzed the membrane proteins of macrophages (3,10,16,17,25,34), neutrophils (1,15,27,30,32,33) and eosinophils (2,28) with one-dimensional SDS PAGE. Yin et al (34) have demonstrated differences in the plasma membrane protein composition between resident and activated macrophages, Remold-O'Donnell (25) has examined trypsin-sensitive surface proteins, and Muller et al (16,17) have studied the effects of the antibody-independent phagocytosis of latex beads on the membrane proteins of murine THE JOURNAL OF CELL BIOLOGY " VOLUME 92 February 1982 283-288 © The Rockefeller University Press -0021-9525/82/02/0283/06 $1 .00 macrophages .…”

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confidence: 99%

Identification of phagocytosis-associated surface proteins of macrophages by two-dimensional gel electrophoresis.

Howard¹,

Petty²,

McConnell³

1982

The Journal of Cell Biology

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Two-dimensional PAGE (P . Z. O'Farrell, H. M. Goodman, and P . H . O'Farrell . 1977 . Cell . 12 :1133-1142 has been employed to assess the effects of antibody-dependent phagocytosis on the cell surface protein composition of RAW264 macrophages. Unilamellar phospholipid vesicles containing 1% dinitrophenyl-aminocaproyl-phosphatidylethanol amine (DNPcap-PE) were used as the target particle . Macrophages were exposed to anti-DNP antibody alone, vesicles alone, or vesicles in the presence of antibody for 1 h at 37°C . Cell surface proteins were then labeled by lactoperoxidase-catalyzed radioiodination at 4°C. After detergent solubilization, membrane proteins were analyzed by two-dimensional gel electrophoresis. The resulting pattern of spots was compared to that of standard proteins . We have identified several surface proteins, not apparently associated with the phagocytic process, which are present either in a multichain structure or in several discretely charged forms. After phagocytosis, we have observed the appearance of two proteins of 45 and 50 kdaltons in nonreducing gels . In addition, we have noted the disappearance of a 140-kdalton protein in gels run under reducing conditions . These alterations would not be detected in the conventional one-dimensional gel electrophoresis. This evidence shows that phagocytosis leads to a modification of cell surface protein composition . Our results support the concept of specific enrichment and depletion of membrane components during antibody-dependent phagocytosis .

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Diversidade morfológica, biológica e genética, e relações filogenéticas de tripanossomas de morcegos do Brasil e Moçambique (África).

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Eosinophil membrane changes during interaction with antibody-coated non-phagocytosable surfaces

Cited by 3 publications

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Heterogeneity of Human Peripheral Blood Eosinophils: Variability in Cell Density and Cytotoxic Ability in Relation to the Level and the Origin of Hypereosinophilia

Heterogeneity of Human Peripheral Blood Eosinophils: Variability in Cell Density and Cytotoxic Ability in Relation to the Level and the Origin of Hypereosinophilia

Identification of phagocytosis-associated surface proteins of macrophages by two-dimensional gel electrophoresis.

Diversidade morfológica, biológica e genética, e relações filogenéticas de tripanossomas de morcegos do Brasil e Moçambique (África).

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