Eosinophilic granule cells in Carassius auratus scale epidermis

Abstract

DePasquale, J.A. 2017. Eosinophilic granule cells in Carassius auratus scale epidermis. -Acta Zoologica (Stockholm) 98: 387-399.Eosinophilic granule cells (EGCs) were characterized in Carassius auratus scale epidermis in situ and in explants. Live EGCs were readily identified by the presence of numerous large cytoplasmic granules observed with DIC microscopy. Histochemical staining with toluidine blue and alcian blue yielded granule metachromasia and pale blue granules, respectively, both consistent with mammalian mast cell staining. However, EGCs also share some features with mammalian basophils as neutral red dye was selectively incorporated into EGC granules. EGCs within scale epidermis were actively motile, displaying average speeds of 16 lm/min and maximum speeds of greater than 40 lm/min and showing morphological plasticity during migration. The predominant motile phenotype was elongate with a well-developed leading lamella, while a broader body motile morphology was observed to a lesser extent. A trailing, relatively unchanged uropod was associated with every motile EGC and invariably contained one or a few granules. A rounded EGC shape without a leading-edge or trailing uropod was also observed and was generally associated with static cells. Individual cells readily switched between the three major shapes during motility; static cells could abruptly develop a polarized morphology, and actively motile cells switched between elongate and broad-bodied shapes or the static, rounded shape.EGCs in goldfish skin DePasquale Acta Zoologica (Stockholm) 98: 387-399 (October 2017)

Density, Distribution and Staining Properties of Eosinophilic Granular Cells in Oscar Fish (Astronotus ocellatus) Intestine

“…They are cells of hematopoietic lineage that play a defensive role in parasitic and microbial infections (Penissi et al, 2003;Marshall, 2004). Studies on fish EGCs have revealed that these cells generate a heterogeneous group of cells (Reite, 1998;Reite and Evensen, 2006;Hopperdietzel et al,2015;DePasquale, 2017). This heterogeneity ascribes to overt basophilic or eosinophilic staining characteristics of granules that have been identified in various species.…”

“…This heterogeneity ascribes to overt basophilic or eosinophilic staining characteristics of granules that have been identified in various species. Therefore, those cells have been named as mast cells, basophilic granular cells, or acidophilic/eosinophilic granular cells in distinct vertebrate species studied up to now (Ellis, 1977;Reite, 1998;Hopperdietzel et al, 2015;DePasquale, 2017).…”

“…Basophilic and eosinophilic (acidophilic) components present in the granules of EGCs may exhibit great differences between fish species, even fish families (Reite,1996;Reite,1997;Hopperdietzel et al, 2015;DePasquale, 2017). Some studies have reported the existence of many EGCs in some fish families (Temkin and McMillan, 1986;Vallejo and Ellis, 1989;Dorin et al, 1993;Hopperdietzel et al, 2015;DePasquale, 2017) whereas other studies have shown the lack of EGCs or few in some fish families (Sis et al, 1979;Buddington and Doroshov, 1986;Williams and Nichol, 1989;Reite, 2005). For example, eosinophilic components are predominant in EGCs of labrids (Reite, 1995), while both basophilic and eosinophilic components are found in salmonids (Reite, 1997).…”

“…Therefore, an approach to Giemsa, thionine and toluidine blue, alcian blue / safranin O staining of sections fixed with different fixative solutions offers a practical and cheap alternative to the distinct immunohistochemical (Dezfuli et al, 2002) or cytochemical (Da'as et al, 2011) detection approaches of intestinal EGCs in fish. In this regard, the literature reports the results unique to each type of staining and fixation (Hopperdietzel et al, 2015;DePasquale, 2017). Studies comparing different fixatives and staining procedures have reported that EGCs in carp and trout tissues fixed with alcohol-based solutions were stained metachromatically with thionine (Reite and Evensen, 1994;Reite, 1997).…”

“…They are involved in early immune responses, and bear granules with different staining properties (Wernersson and Pejler, 2014). Various stimuli cause them to degranulate and release their contents (DePasquale, 2017).…”