Eosinophils and neutrophils in biopsies from the middle ear of atopic children with otitis media with effusion

Amin, Kawa; Hurst, David S.; Roomans, G. M.; Sevéus, Lahja

doi:10.1007/s000110050513

Cited by 14 publications

(9 citation statements)

References 27 publications

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“…EPO encodes erythropoietin, a protein that controls erythropoiesis and production of red blood cells [33]. In addition, the middle ear in subjects having OM with effusion demonstrated elevated levels of EPO, eosinophils and neutrophils [34]. Allergic inflammation with elevated eosinophils in the middle ear can cause OM with effusion [35].…”

Section: Discussionmentioning

confidence: 99%

Identification of Potential Novel Biomarkers and Signaling Pathways Related to Otitis Media Induced by Diesel Exhaust Particles Using Transcriptomic Analysis in an In Vivo System

Kim

Kwon

et al. 2016

PLoS ONE

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IntroductionAir pollutants are associated with inflammatory diseases such as otitis media (OM). Significantly higher incidence rates of OM are reported in regions with air pollution. Diesel exhaust particles (DEPs) comprise a major class of contaminants among numerous air pollutants, and they are characterized by a carbonic mixture of polycyclic aromatic hydrocarbons (PAHs), nitro-PAHs, and small amounts of sulfate, nitrate, metals and other trace elements. DEP exposure is a risk factor for inflammatory diseases. Our previous study identified potential biomarkers using gene expression microarray and pathway analyses in an in vitro system. Although in vitro investigations have been conducted to elucidate plausible biomarkers and molecular mechanisms related to DEP exposure, in vivo studies are necessary to identify the exact biological relevance regarding the incidence of OM caused by DEP exposure. In this study, we identified potential molecular biomarkers and pathways triggered by DEP exposure in a rodent model.MethodsTranscriptomic analysis was employed to identify novel potential biomarkers in the middle ear of DEP-exposed mice.ResultsA total of 697 genes were differentially expressed in the DEP-exposed mice; 424 genes were upregulated and 273 downregulated. In addition, signaling pathways among the differentially expressed genes mediated by DEP exposure were predicted. Several key molecular biomarkers were identified including cholinergic receptor muscarinic 1 (CHRM1), erythropoietin (EPO), son of sevenless homolog 1 (SOS1), estrogen receptor 1 (ESR1), cluster of differentiation 4 (CD4) and interferon alpha-1 (IFNA1).ConclusionsOur results shed light on the related cell processes and gene signaling pathways affected by DEP exposure. The identified biomarkers might be potential candidates for determining early diagnoses and effective treatment strategies for DEP-mediated disorders.

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Section: Discussionmentioning

confidence: 99%

Identification of Potential Novel Biomarkers and Signaling Pathways Related to Otitis Media Induced by Diesel Exhaust Particles Using Transcriptomic Analysis in an In Vivo System

Kim

Kwon

et al. 2016

PLoS ONE

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“…Histopathologic and biochemical studies confirmed the presence of these cell types in the effusion and in the subepithelial space of the middle ear cleft (13). Amin et al (14) described neutrophils and eosinophils in biopsies from middle ear mucosa using an immunohistochemistry method. Hurst and Verge (6,15) verified the presence of eosinophils, mast cells, and neutrophils by measuring the levels of the cell-specific enzymes in the effusion.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Otitis Media With Effusion Based on the Ratio of Albumin and Immunoglobulin G Concentrations in the Effusion

Rezes

Késmárki

Sipka

et al. 2007

Otology &Amp; Neurotology

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“…22 Messenger RNA for IL-8 is actively produced in the cells found in MEE associated with viral infections but is not found to arise from mucosal cells. 23 Biopsy studies demonstrate a greater number of eosinophils and neutrophils in the mucus than in the mucosa of ears of atopic patients with chronic OME and a significant and similar ratio of neutrophils and eosinophils both in the mucus and mucosa, 24 indicating a proportional influx by both cells into the middle ear of atopic patients.…”

Section: Commentmentioning

confidence: 94%

The Impact of Atopy on Neutrophil Activity in Middle Ear Effusion From Children and Adults With Chronic Otitis Media

Hurst

2002

Arch Otolaryngol Head Neck Surg

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The surprising finding of marked elevation of effusion MPO in atopic patients but very low levels in nonatopic patients (P < .001) suggests that atopy may contribute to elevated levels of neutrophil activity in OME. An atopic patient may respond differently from a nonatopic one to the microbial or viral products of acute inflammation owing to the presence of primed inflammatory cells. This study provides confirmation on a cellular level that neutrophils are an integral part of the inflammatory process in OME to a disproportionate degree among atopic patients.

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Eosinophils and neutrophils in biopsies from the middle ear of atopic children with otitis media with effusion

Abstract: The results of this study show the feasibility of using specific antibodies to identify eosinophils and neutrophils in the middle ear. The initial data suggest that atopic children with OME have higher numbers of such cells as compared to non-OME controls.

Cited by 14 publications

References 27 publications

Identification of Potential Novel Biomarkers and Signaling Pathways Related to Otitis Media Induced by Diesel Exhaust Particles Using Transcriptomic Analysis in an In Vivo System

Identification of Potential Novel Biomarkers and Signaling Pathways Related to Otitis Media Induced by Diesel Exhaust Particles Using Transcriptomic Analysis in an In Vivo System

Characterization of Otitis Media With Effusion Based on the Ratio of Albumin and Immunoglobulin G Concentrations in the Effusion

The Impact of Atopy on Neutrophil Activity in Middle Ear Effusion From Children and Adults With Chronic Otitis Media

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