Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy

Dorosz, Aleksandra; Grosicki, Marek; Dybaś, Jakub; Matuszyk, Ewelina; Rodewald, M.; Meyer, Tobias; Popp, Jürgen; Malek, Kamilla; Barańska, Małgorzata

doi:10.3390/cells9092041

Cited by 21 publications

(17 citation statements)

References 52 publications

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“…The existence of a huge number of immature cells in HUCB has also been reported [ 6 ]. In this study, granulocytes, including band cells, basophils, eosinophils, and neutrophils, showed ultrastructural characteristics similar to those in humans, as described by some authors [ 7 - 10 ]. Human and UCB cells have similar structures, suggesting that bovine umbilical cords could constitute a model for human umbilical cord research and medication-based therapy for stem cell and cell-free therapy.…”

Section: The Structure Of Bovine Umbilical Cordssupporting

confidence: 87%

Conditioned medium derived from bovine umbilical mesenchymal stem cells as an alternative source of cell-free therapy

Kusindarta

Wihadmadyatami

2021

Vet World

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Umbilical cord blood (UCB) cells are an important source of mesenchymal stem cells (MSCs). It is known that the umbilical cord is rich in hematopoietic stem cells, which influenced research on ontogeny and transplantation (allogeneic transplantation). In recent years, stem cell research has emerged as an area of major interest due to its prospective applications in various aspects of both human and veterinary medicine. Moreover, it is known that the application of MSCs has several weaknesses. The use of these cells has limitations in terms of tumorigenesis effect, delivery, safety, and variability of therapeutic response, which led to the use of secretomes as an alternative to cell-free therapy. The main obstacle in its use is the availability of human UCB as an origin of MSCs and MSCs' secretomes, which are often difficult to obtain. Ethical issues regarding the use of stem cells based on human origin are another challenge, so an alternative is needed. Several studies have demonstrated that MSCs obtained from bovine umbilical cords have the same properties and express the same surface markers as MSCs obtained from human umbilical cords. Therefore, secretomes from MSCs derived from domestic animals (bovine) can possibly be used in human and veterinary medicine. This finding would contribute significantly to improve cell-free therapy. At present, the use of UCB MSCs derived from domestic animals, especially bovines, is very restricted, and only limited data about bovine UCB are available. Therefore, the aim of this review was to provide an updated overview of cell-free therapy and discuss the new possibilities introduced by the generation of this therapy derived from bovine umbilical MSCs as a promising tool in developing modern and efficient treatment strategies.

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Section: The Structure Of Bovine Umbilical Cordssupporting

confidence: 87%

Conditioned medium derived from bovine umbilical mesenchymal stem cells as an alternative source of cell-free therapy

Kusindarta

Wihadmadyatami

2021

Vet World

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“…Commonly, eosinophils are a kind of white blood cells, measuring 10-20 µm in diameter, with a bilobed nucleus [4] (Figures 1 and 2). In homeostatic situations, they circulate in the bloodstream ranging from 0 to 500 eosinophils per microliter [5].…”

Section: Unraveling the Complex Biology Of Eosinophils: From Development To Trafficmentioning

confidence: 99%

Emerging Evidence for Pleiotropism of Eosinophils

Rodrigo‐Muñoz

Gil-Martínez

Sastre

et al. 2021

IJMS

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Eosinophils are complex granulocytes with the capacity to react upon diverse stimuli due to their numerous and variable surface receptors, which allows them to respond in very different manners. Traditionally believed to be only part of parasitic and allergic/asthmatic immune responses, as scientific studies arise, the paradigm about these cells is continuously changing, adding layers of complexity to their roles in homeostasis and disease. Developing principally in the bone marrow by the action of IL-5 and granulocyte macrophage colony-stimulating factor GM-CSF, eosinophils migrate from the blood to very different organs, performing multiple functions in tissue homeostasis as in the gastrointestinal tract, thymus, uterus, mammary glands, liver, and skeletal muscle. In organs such as the lungs and gastrointestinal tract, eosinophils are able to act as immune regulatory cells and also to perform direct actions against parasites, and bacteria, where novel mechanisms of immune defense as extracellular DNA traps are key factors. Besides, eosinophils, are of importance in an effective response against viral pathogens by their nuclease enzymatic activity and have been lately described as involved in severe acute respiratory syndrome coronavirus SARS-CoV-2 immunity. The pleiotropic role of eosinophils is sustained because eosinophils can be also detrimental to human physiology, for example, in diseases like allergies, asthma, and eosinophilic esophagitis, where exosomes can be significant pathophysiologic units. These eosinophilic pathologies, require specific treatments by eosinophils control, such as new monoclonal antibodies like mepolizumab, reslizumab, and benralizumab. In this review, we describe the roles of eosinophils as effectors and regulatory cells and their involvement in pathological disorders and treatment.

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“…Further studies combining fluorescence tags and RS include bladder cancer cells expressing the widely used GFP to monitor the distribution of proteins in living cells 74 and the investigation of biochemical features of immune cells eosinophils and neutrophils. 75 In addition to the here reviewed spontaneous RS, FS has also been combined in several studies with surface-enhanced Raman scattering using nanoparticles to enhance the singal. 76 – 79 …”

Section: Optical Modalities For Medical Diagnosticsmentioning

confidence: 99%

Looking for a perfect match: multimodal combinations of Raman spectroscopy for biomedical applications

2021

Self Cite

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. Raman spectroscopy has shown very promising results in medical diagnostics by providing label-free and highly specific molecular information of pathological tissue ex vivo and in vivo . Nevertheless, the high specificity of Raman spectroscopy comes at a price, i.e., low acquisition rate, no direct access to depth information, and limited sampling areas. However, a similar case regarding advantages and disadvantages can also be made for other highly regarded optical modalities, such as optical coherence tomography, autofluorescence imaging and fluorescence spectroscopy, fluorescence lifetime microscopy, second-harmonic generation, and others. While in these modalities the acquisition speed is significantly higher, they have no or only limited molecular specificity and are only sensitive to a small group of molecules. It can be safely stated that a single modality provides only a limited view on a specific aspect of a biological specimen and cannot assess the entire complexity of a sample. To solve this issue, multimodal optical systems, which combine different optical modalities tailored to a particular need, become more and more common in translational research and will be indispensable diagnostic tools in clinical pathology in the near future. These systems can assess different and partially complementary aspects of a sample and provide a distinct set of independent biomarkers. Here, we want to give an overview on the development of multimodal systems that use RS in combination with other optical modalities to improve the diagnostic performance.

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Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy

Cited by 21 publications

References 52 publications

Conditioned medium derived from bovine umbilical mesenchymal stem cells as an alternative source of cell-free therapy

Conditioned medium derived from bovine umbilical mesenchymal stem cells as an alternative source of cell-free therapy

Emerging Evidence for Pleiotropism of Eosinophils

Looking for a perfect match: multimodal combinations of Raman spectroscopy for biomedical applications

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