Eosinophils in human oral squamous carcinoma; role of prostaglandin D2

Abstract

Eosinophils are often predominant inflammatory leukocytes infiltrating oral squamous carcinoma (OSC) sites. Prostaglandins are secreted by oral carcinomas and may be involved in eosinophil infiltration. The objective of this study was to determine the factors contributing to eosinophil migration and potential anti-neoplastic effects on OSC. Eosinophil degranulation was evaluated by measuring release of eosinophil peroxidase (EPO). Eosinophil chemotaxis towards OSC cells was assessed using artificial basement membrane. Eosinophil infiltration was prominent within the tissue surrounding the OSC tumor mass. We observed growth inhibition of the OSC cell line, SCC-9, during co-culture with human eosinophils, in vitro, which correlated with EPO activity that possesses growth inhibitory activity. The PGD2 synthase inhibitor, HQL-79, abrogated migration towards SCC-9. Our data suggest that OSC-derived PGD2 may play an important role via CRTH2 (the PGD2 receptor on eosinophils) in eosinophil recruitment and subsequent anti-tumor activity through the action of eosinophil cationic proteins.

In Utero exposure to genistein enhanced intranasal house dust mite allergen-induced respiratory sensitization in young adult B6C3F1 mice

“…The EPO assay is a colorimetric assay used for detecting eosinophils by measuring the eosinophil peroxidase activity (Strath et al, 1985; Davoine et al, 2013). To measure eosinophil peroxidase activity in the undiluted BALF (100 μl), Tris-HCl buffer (50 μl) was added to 96-well plates initially.…”

Eosinophils and Cancer – Mini Review

“…In oral squamous cell carcinoma with tumor-associated tissue eosinophilia (TATE), the source of CCL11/eotaxin is apparently eosinophils [26]. The cultured oral squamous cell carcinoma cell line SCC9 secretes chemotactic prostaglandin D2, which promotes eosinophil transmigration, thus providing a signal to recruit eosinophils into tumor masses [27]. Eosinophils that infiltrate into tumors/ lymphomas also express IL-6, TGFβ, and CCL24/eotaxin-2 [28].…”

Eosinophils in health and diseases

“…Eosinophils have been found to be located in intratumoral and peritumoral site in various cancers apart from oral cancers such as cancers of larynx, pharynx, lung, intestine, and genitourinary tract. [8] Kargahi et al in their study compared the quantity of tumor-associated tissue eosinophils (TATE) in normal tissue and dysplastic tissue and observed rise in the quantity of eosinophils in dysplasia compared to normal tissue. It was also seen that number of TATE increased as the severity of dysplasia increased.…”