Eosinophils isolated with two different methods show different characteristics of activation

Blom, M.T.; Tool, Anton T.J.; Mul, Frederik P. J.; Knol, Edward F.; Roos, Dirk; Verhoeven, Arthur J.

doi:10.1016/0022-1759(94)00255-u

Cited by 36 publications

(17 citation statements)

References 34 publications

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“…Subsequently, the eosinophils were purified by centrifugation over isotonic Percoll (1.082 g/ml). This purification method does not induce activation of eosinophils, but rather selects a population of resting eosinophils [19]. When eosinophils isolated via density centrifugation [18] were compared to eosinophils isolated by selection of CD16-negative granulocytes [20], comparable degranulation was found (data not shown).…”

Section: Methodsmentioning

confidence: 75%

Eosinophil Granulocyte Interaction with Serum-Opsonized Particles: Binding and Degranulation Are Enhanced by Tumor Necrosis Factor Alpha

Egesten¹,

Blom²,

Calafat³

et al. 1998

Int Arch Allergy Immunol

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Eosinophils participate in the inflammatory response seen in allergy and helminthic infestation. Their release of granule-bound cationic proteins may play a role in these diseases. Therefore, we investigated mechanisms involved in the release of eosinophil cationic protein (ECP). Serum-opsonized zymosan was phagocytosed by eosinophils, and ECP was released into the phagosomes as judged by immunoelectron microscopy. Degranulation to the external milieu was induced by serum-opsonized, non-phagocytosable Sephadex beads (SOS), and ECP release was determined by use of an enzyme-linked immunosorbent assay. CD11b, CD18, and CD32 monoclonal antibodies inhibited degranulation, demonstrating dependence on complement receptor type 3 (CR3), and the low-affinity Fc receptor for IgG. Tumor necrosis factor-α (TNF-α) and interleukin (IL)-5 both rapidly enhanced the binding of eosinophils to serum-opsonized zymosan, and also the release of ECP upon interaction with SOS. The cytokine-induced increase in ECP release was inhibited by the phospholipase A₂ (PLA₂) inhibitor mepacrine, indicating an involvement of PLA₂ in the enhanced response but not in baseline degranulation. Autocrine stimulation by the platelet-activating factor (PAF) is unlikely since the PAF receptor antagonist WEB 2086 did not inhibit the enhanced response. In conclusion, the main signals for eosinophil degranulation on serum-opsonized particles are mediated by CR3 and receptors for immunoglobulins. As for IL-5, TNF-α changes eosinophil phenotype from a resting to an activated state.

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Section: Methodsmentioning

confidence: 75%

Eosinophil Granulocyte Interaction with Serum-Opsonized Particles: Binding and Degranulation Are Enhanced by Tumor Necrosis Factor Alpha

Egesten¹,

Blom²,

Calafat³

et al. 1998

Int Arch Allergy Immunol

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“…Purity of eosinophils was Ͼ95%. This procedure leads to the isolation of relatively unprimed eosinophils compared with conventionally used isolation procedures with immunomagnetic beads (29).…”

Section: Isolation Of Eosinophilsmentioning

confidence: 99%

IL-8 Induces a Transient Arrest of Rolling Eosinophils on Human Endothelial Cells

Ulfman

Joosten

Linden

et al. 2001

The Journal of Immunology

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Eosinophils exhibit a rolling interaction with E-selectin-expressing endothelium, and need to be activated by inflammatory mediators to firmly adhere to this surface. This study shows that IL-8 induces a transient arrest of unprimed eosinophils that roll on E-selectin present on TNF-α-activated HUVEC in an in vitro flow chamber. This process was antagonized by neutralizing Abs directed against IL-8 showing the specificity of the IL-8 effect. Furthermore, blocking Abs against both α4 and β2 integrins inhibited the IL-8-induced transient arrest while these Abs had no effect when they were added separately. The IL-8-induced arrest was pertussis toxin sensitive. Studying the effect of IL-8 in more detail, we evaluated putative changes in intracellular Ca2+ concentration in eosinophils induced by IL-8. We could show that IL-8 induces a transient rise in intracellular Ca2+ concentration in ∼40% of the cells provided that the eosinophils are interacting with endothelial cells or fibronectin-coated surfaces. Together these data show that resting eosinophils respond to IL-8 provided that the cells adhere on physiological surfaces. The induction of a transient arrest provides a new level of chemokine-induced regulation of leukocyte adhesion under flow conditions.

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“…The decrease in intracellular CD9 expression was more pronounced in purified eosinophils. This may be associated to a priming of eosinophils during the purification step, making them more prone to subsequent stimuli [23].…”

Section: Discussionmentioning

confidence: 99%

The effect of in vitro activation and platelet interaction on the CD9 distribution and adhesion properties of human eosinophils

Fernvik¹,

Lundahl²,

Magnusson³

et al. 1999

Inflammation Research

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Eosinophils isolated with two different methods show different characteristics of activation

Cited by 36 publications

References 34 publications

Eosinophil Granulocyte Interaction with Serum-Opsonized Particles: Binding and Degranulation Are Enhanced by Tumor Necrosis Factor Alpha

Eosinophil Granulocyte Interaction with Serum-Opsonized Particles: Binding and Degranulation Are Enhanced by Tumor Necrosis Factor Alpha

IL-8 Induces a Transient Arrest of Rolling Eosinophils on Human Endothelial Cells

The effect of in vitro activation and platelet interaction on the CD9 distribution and adhesion properties of human eosinophils

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