Eotaxin-2 in sputum cell culture to evaluate asthma inflammation

Scheicher, Marcos Eduardo; Teixeira, Mauro Martins; Cunha, Fernando; Teixeira, André Luís de Souza; Filho, José Teixeira; Vianna, Elcio Oliveira

doi:10.1183/09031936.00060205

Cited by 20 publications

(14 citation statements)

References 41 publications

(39 reference statements)

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“…In our study, there was a significant correlation between eotaxin levels and percentage eosinophils in patients with asthma. These results concur with Scheicher et al.’s findings (24). Fujisawa et al.…”

Section: Discussionsupporting

confidence: 94%

The roles of a Th2 cytokine and CC chemokine in children with stable asthma: Potential implication in eosinophil degranulation

Kim

Kita

Callaway

et al. 2010

Pediatric Allergy and Immunology

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Th2 cytokine IL-5 and CC chemokine eotaxin are thought to be key regulators of eosinophils in bronchial asthma. However, their involvement in children with stable asthma (SA) has not been determined. We investigated the roles of IL-5 and eotaxin in eosinophil degranulation in children with SA. Induced sputum was obtained from 30 SA, 21 allergic rhinitis (AR), and 22 non-atopic healthy control (HC) children. We measured sputum levels of IL-5, eotaxin, and eosinophil indices [percentage eosinophils, eosinophil-derived neurotoxin (EDN), and eosinophil- cationic protein (ECP)]. We also examined correlations of IL-5 and eotaxin with eosinophil indices. Sputum percentage eosinophils and EDN and ECP levels were significantly higher in the SA group than in the HC group, while only the sputum EDN and ECP levels were significantly higher in the AR group than in the HC group. Unexpectedly, sputum levels of IL-5 were not significantly different among the three groups; however, the levels of eotaxin were higher in the SA group when compared to the HC group. No significant correlations were found between IL-5 and percentage eosinophils, EDN, or ECP levels; in contrast, eotaxin levels correlated significantly with percentage eosinophils (R= 0.638; p = 0.0001), EDN (R= 0.522; p = 0.003), and ECP levels (R= 0.630 and p = 0.0002). The elevated levels and good correlations of eotaxin with sputum eosinophil indices, and no elevation or correlation of IL-5 with these indices, suggest that CC chemokine eotaxin may play a more important role in eosinophil degranulation in children with SA.

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Section: Discussionsupporting

confidence: 94%

The roles of a Th2 cytokine and CC chemokine in children with stable asthma: Potential implication in eosinophil degranulation

Kim

Kita

Callaway

et al. 2010

Pediatric Allergy and Immunology

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“…This sustained expression is surprising given the reported suppression of IL-4/IL-13-stimulated eotaxins by CS in vitro and in those with mild asthma treated with oral CS. 113031 To address this, IL-13-treated epithelial cells were treated with dexamethasone for 48 h. Although IL-13-stimulated eotaxin-2 and eotaxin-3 protein were consistently suppressed by CSs, the overall decrease was small (25%). Thus, although in simple cell culture systems there is consistent suppression, the translation of this to the more complicated in vivo setting is unclear.…”

Section: Discussionmentioning

confidence: 99%

Epithelial eotaxin-2 and eotaxin-3 expression: relation to asthma severity, luminal eosinophilia and age at onset

Coleman

Naik

Holguín

et al. 2012

Thorax

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Background Eosinophilic inflammation is implicated in asthma. Eotaxin 1–3 regulate eosinophil trafficking into the airways along with other chemotactic factors. However, the epithelial and bronchoalveolar lavage (BAL) cell expression of these chemokines in relation to asthma severity and eosinophilic phenotypes has not been addressed. Objective To measure the expression of the three eotaxin isoforms in bronchoscopically obtained samples and compare them with clinically relevant parameters between normal subjects and patients with asthma. Methods Normal subjects and patients with asthma of varying severity recruited through the Severe Asthma Research Program underwent clinical assessment and bronchoscopy with airway brushing and BAL. Eotaxin 1–3 mRNA/protein were measured in epithelial and BAL cells and compared with asthma severity, control and eosinophilic inflammation. Results Eotaxin-2 and eotaxin-3 mRNA and eotaxin-2 protein were increased in airway epithelial brushings from patients with asthma and were highest in cases of severe asthma (p values 0.0155, 0.0033 and 0.0006, respectively), with eotaxin-2 protein increased with age at onset. BAL cells normally expressed high levels of eotaxin-2 mRNA/protein but BAL fluid levels of eotaxin-2 were lowest in severe asthma. Epithelial eotaxin-2 and eotaxin-3 mRNA/protein was associated with sputum eosinophilia, lower forced expiratory volume in 1 s and more asthma exacerbations. Airway epithelial cell eotaxin-2 protein differed by asthma severity only in those with late onset disease, and tended to be highest in those with late onset eosinophilic asthma. Conclusions Epithelial eotaxin-2 and 3 are increased in asthma and severe asthma. Their expression may contribute to luminal migration of eosinophils, especially in later onset disease, asthma control and severity.

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“…11,12 Eotaxin is a key mediator for intrapulmonary eosinophil accumulation and development of lung injury. Eotaxin-2/CCL24 is a potent eosinophil chemoattractant in vitro and in vivo ,13–15 similarly to eotaxin-1,16 and is increased in sputum cell cultures from patients with asthma 17. More recently, a range of non-CCR3-binding chemokines, which are able to influence eosinophil function, has been identified, including CCL3 (macrophage inflammatory protein-1α) and CCL22 (macrophage-derived chemokine) 18.…”

Section: Discussionmentioning

confidence: 99%