Epalrestat-loaded silicone hydrogels as contact lenses to address diabetic-eye complications

Alvarez-Rivera, Fernando; Concheiro, Ángel

doi:10.1016/j.ejpb.2017.10.016

Cited by 66 publications

(54 citation statements)

References 37 publications

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“…Bovine tests showed drug accumulation within the cornea. Reproduced with permission . Copyright, Elsevier.…”

Section: Applicationsmentioning

confidence: 99%

“…Drug nanosuspensions were loaded in a contact lens for delivery of triamcinolone acetonide, showing significantly increased drug load capacity and releasing times . Silicone hydrogel contact lenses functionalized with epalrestat were successful in addressing diabetic eye complications and cataract, by inhibiting aldose reductase (AR) and preventing protein glycation (Figure b) . Nanogels were used as timolol maleate carriers and loaded into enzyme‐responsive contact lenses, for tear lysozyme‐activated release of timolol maleate for the treatment of glaucoma (Figure c) .…”

Section: Applicationsmentioning

confidence: 99%

See 1 more Smart Citation

Contact Lens Technology: From Fundamentals to Applications

Moreddu

Vigolo

Yetisen

2019

Adv Healthcare Materials

190

171

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Contact lenses are ocular prosthetic devices used by over 150 million people worldwide. Primary applications of contact lenses include vision correction, therapeutics, and cosmetics. Contact lens materials have significantly evolved over time to minimize adverse effects associated with contact lens wearing, to maintain a regular corneal metabolism, and to preserve tear film stability. This article encompasses contact lens technology, including materials, chemical and physical properties, manufacturing processes, microbial contamination, and ocular complications. The function and the composition of the tear fluid are discussed to assess its potential as a diagnostic media. The regulatory standards of contact lens devices with regard to biocompatibility and contact lens market are presented. Future prospects in contact lens technology are evaluated, with particular interest given to theranostic applications for in situ continuous monitoring the ocular physiology.

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“…Bovine tests showed drug accumulation within the cornea. Reproduced with permission . Copyright, Elsevier.…”

Section: Applicationsmentioning

confidence: 99%

Section: Applicationsmentioning

confidence: 99%

Contact Lens Technology: From Fundamentals to Applications

Moreddu

Vigolo

Yetisen

2019

Adv Healthcare Materials

190

171

View full text Add to dashboard Cite

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“…where Vs is the volume of water sorbed by the hydrogel, Vp the volume of dried polymer, Wp the dried hydrogel weight, and C 0 the concentration of viral vector in the loading solution. Finally, the functional monomer factor (FMF) [31] was calculated as the ratio between amounts of rAAV loaded by functionalized hydrogels divided by the amount of rAAV loaded by the HEMA hydrogel without APMA.…”

Section: Incorporation Of Raav Vectors To Hydrogelsmentioning

confidence: 99%

“…Contact lenses (CLs) attract great interest as drug delivery platforms able to provide sustained levels to the post-lens lachrymal fluid, i.e., the volume confined between the CL and the cornea. Attenuated turnover of the post-lens lachrymal fluid facilitates the reaching of higher drug concentrations that prompt the diffusion through the cornea, while non-productive absorption is minimized [28][29][30][31]. Safety of CLs for prolonged wearing to correct vision suggests that they can be considered as patient-friendly drug delivery devices.…”

Section: Introductionmentioning

confidence: 99%

Controlled Release of rAAV Vectors from APMA-Functionalized Contact Lenses for Corneal Gene Therapy

et al. 2020

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As an alternative to eye drops and ocular injections for gene therapy, the aim of this work was to design for the first time hydrogel contact lenses that can act as platforms for the controlled delivery of viral vectors (recombinant adeno-associated virus, rAAV) to the eye in an effective way with improved patient compliance. Hydrogels of hydroxyethyl methacrylate (HEMA) with aminopropyl methacrylamide (APMA) (H1: 40, and H2: 80 mM) or without (Hc: 0 mM) were synthesized, sterilized by steam heat (121 °C, 20 min), and then tested for gene therapy using rAAV vectors to deliver the genes to the cornea. The hydrogels showed adequate light transparency, oxygen permeability, and swelling for use as contact lenses. Loading of viral vectors (rAAV-lacZ, rAAV-RFP, or rAAV-hIGF-I) was carried out at 4 °C to maintain viral vector titer. Release in culture medium was monitored by fluorescence with Cy3-rAAV-lacZ and AAV Titration ELISA. Transduction efficacy was tested through reporter genes lacZ and RFP in human bone marrow derived mesenchymal stem cells (hMSCs). lacZ was detected with X-Gal staining and quantified with Beta-Glo®, and RFP was monitored by fluorescence. The ability of rAAV-hIGF-I-loaded hydrogels to trigger cell proliferation in hMSCs was evaluated by immunohistochemistry. Finally, the ability of rAAV-lacZ-loaded hydrogels to transduce bovine cornea was confirmed through detection with X-Gal staining of β-galactosidase expressed within the tissue.

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“…Aldose reductase is known to cause cataract and retinopathy. Silicone hydrogels were capable of maintaining a sustained Epalrestat release over 7 days, whereas the drug permeated and accumulated in situ , after bovine corneas were treated with hydrogels under hyperlycemic conditions (Alvarez‐Rivera, Concheiro, & Alvarez‐Lorenzo, ).…”

Section: Hydrogels For Treatment Of Diabetes‐related Conditionsmentioning

confidence: 99%

Advanced hydrogels for treatment of diabetes

Reyes-Martínez

Ruíz-Pacheco²,

Flores-Valdéz

et al. 2019

J Tissue Eng Regen Med

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Diabetes mellitus is a chronic disease characterized by high levels of glucose in the blood, which leads to metabolic disorders with severe consequences. Today, there is no cure for diabetes. The current management for diabetes and derived medical conditions, such as hyperglycemia, cardiovascular diseases, or diabetic foot ulcer, includes life style changes and hypoglycemia‐based therapy, which do not fully restore euglycemia or the functionality of damaged tissues in patients. This encourages scientists to work outside their boundaries to develop routes that can potentially tackle such metabolic disorders. In this regard, acellular and cellular approaches have represented an alternative for diabetics, although such treatments still face shortcomings related to limited effectiveness and immunogenicity. The advent of biomaterials has brought significant improvements for such approaches, and three‐dimensional extracellular matrix analogs, such as hydrogels, have played a key role in this regard. Advanced hydrogels are being developed to monitor high blood glucose levels and release insulin, as well as serve as a therapeutic technology. Herein, the state of the art in advanced hydrogels for improving treatment of diabetes, from laboratory technology to commercial products approved by drug safety regulatory authorities, will be concisely summarized and discussed.

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Epalrestat-loaded silicone hydrogels as contact lenses to address diabetic-eye complications

Cited by 66 publications

References 37 publications

Contact Lens Technology: From Fundamentals to Applications

Contact Lens Technology: From Fundamentals to Applications

Controlled Release of rAAV Vectors from APMA-Functionalized Contact Lenses for Corneal Gene Therapy

Advanced hydrogels for treatment of diabetes

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