EPCR Ser219Gly: Elevated sEPCR, prothrombin F1+2, risk for coronary heart disease, and increased sEPCR shedding in vitro

Ireland, H; Konstantoulas, Constantine James; Cooper, Jackie A.; Hawe, Emma; Humphries, Steve E.; Mather, H.; Goodall, Alison H.; Hogwood, John; Juhan‐Vague, I.; Yudkin, John; Minno, G. Di; Margaglione, Maurizio; Hamsten, Anders; Miller, George; Bauer, Kenneth A.; Kim, Y.T.; Stearns-Kurosawa, Deborah J.

doi:10.1016/j.atherosclerosis.2005.02.028

Cited by 58 publications

(91 citation statements)

References 34 publications

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“…The 4678C allele (A1 haplotype) and the 4600G allele (A3 haplotype) frequencies for the control group (0.468 and 0.103, respectively) were in the middle of the frequency range reported by other groups (0.480 15 18 for the A3 haplotype). Both the A3 and the A1 haplotypes were more frequent in control subjects than in MI patients.…”

Section: Epcr Polymorphisms and Myocardial Infarction Riskmentioning

confidence: 64%

“…After adjustment for sex, age and all the other risk factors, both the presence of the A3 allele and the A1 allele were associated with a reduction in the risk of premature MI (Table 3). Ireland et al 18 reported that the apparent protection against coronary heart disease in carriers of the A3 haplotype disappears in the presence of diabetes or metabolic syndrome. We, therefore, analyzed the subgroup of MI patients and controls with diabetes (101 patients and 46 controls).…”

Section: Epcr Polymorphisms and Myocardial Infarction Riskmentioning

confidence: 99%

“…13,14 There are at least four haplotypes in the EPCR gene. Haplotype 3 (A3), tagged by the single nucleotide polymorphism (SNP) 4600A/G (rs 867186), has been reported to be associated with increased plasma levels of soluble EPCR (sEPCR) [15][16][17][18][19] and with increased EPCR shedding in vitro, 18,20 probably due to increased sensitivity to ADAM17, 21 but its association with the risk of venous thromboembolism is unclear. While two studies failed to show an association between the A3 haplotype and the risk of venous thrombosis in Spanish and Dutch subjects, 16,17 this haplotype was overrepresentated in male patients with venous thrombosis in a French study 15 and in carriers of the prothrombin 20210A allele in a Spanish study.…”

Section: Introductionmentioning

confidence: 99%

“…While two studies failed to show an association between the A3 haplotype and the risk of venous thrombosis in Spanish and Dutch subjects, 16,17 this haplotype was overrepresentated in male patients with venous thrombosis in a French study 15 and in carriers of the prothrombin 20210A allele in a Spanish study. 19 Ireland et al, 18 in a pan-European case-control study of MI, reported that without the challenge of diabetes or metabolic syndrome, individuals carrying one A3 haplotype were protected from MI. However, when heterozygotes had factors constituting the metabolic syndrome or diabetes, the risk of MI increased.…”

Section: Introductionmentioning

confidence: 99%

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Endothelial protein C receptor polymorphisms and risk of myocardial infarction

Medina¹,

Navarro²,

Corral³

et al. 2008

Haematologica

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Section: Epcr Polymorphisms and Myocardial Infarction Riskmentioning

confidence: 64%

Section: Epcr Polymorphisms and Myocardial Infarction Riskmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Endothelial protein C receptor polymorphisms and risk of myocardial infarction

Medina¹,

Navarro²,

Corral³

et al. 2008

Haematologica

View full text Add to dashboard Cite

show abstract

“…28 A significantly higher frequency of coronary heart disease was observed in A3 homozygotes, and the A3 haplotype was also associated with an increased risk of coronary heart disease in type-2 diabetic patients.…”

Section: Circumstances Leading To Decreased Membrane Epcr Expression mentioning

confidence: 94%

Endothelial cell protein C receptor and the risk of venous thrombosis

Gandrille¹

2008

Haematologica

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Poly(methyl methacrylate) microchip affinity capillary gel electrophoresis of aptamer–protein complexes for the analysis of thrombin in plasma

et al. 2008

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Thrombin generation in blood serves as an important marker for various hemostasis-related diseases and conditions. Analytical techniques currently utilized for determining the thrombin potential of patients rely primarily on the enzymatic activity of thrombin. Microfluidic-based ACE using fluorescently labeled aptamers as affinity probes could provide a simple and efficient technique for the real-time analysis of thrombin levels in plasma. In this study, aptamers were used for the analysis of thrombin by affinity microchip CGE. The CGE used a poly(methyl methacrylate) (PMMA) microfluidic device for the sorting of the affinity complexes with a linear polyacrylamide (LPA) serving as the sieving matrix. Due to the fact that the assay was run under nonequilibrium electrophoresis conditions, the presence of the sieving gel was found to stabilize the affinity complex, providing improved electrophoretic performance compared to free-solution electrophoresis. Two fluorescently labeled aptamer affinity probes, HD1 and HD22, which bind to exosites I and II, respectively, of thrombin were investigated. With an electric field strength of 300 V/cm, two well-resolved peaks corresponding to free aptamer and the thrombin-aptamer complex were obtained in less than 1 min of separation time with a run-to-run and chip-to-chip reproducibility (RSD) of migration times <10% using both aptamers. HD22 affinity assays of thrombin produced baseline-resolved peaks with favorable efficiency due to its higher binding affinity, whereas HD1 assays showed poorer resolution of the free aptamer and complex peaks. HD22 was used in determining the level of thrombin in human plasma. Assays were performed directly on plasma that was diluted to 10% v/v. Thrombin was successfully analyzed by microchip CGE at a concentration level of 543.5 nM for the human plasma sample.

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EPCR Ser219Gly: Elevated sEPCR, prothrombin F1+2, risk for coronary heart disease, and increased sEPCR shedding in vitro

Cited by 58 publications

References 34 publications

Endothelial protein C receptor polymorphisms and risk of myocardial infarction

Endothelial protein C receptor polymorphisms and risk of myocardial infarction

Endothelial cell protein C receptor and the risk of venous thrombosis

Poly(methyl methacrylate) microchip affinity capillary gel electrophoresis of aptamer–protein complexes for the analysis of thrombin in plasma

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