epg5 knockout leads to the impairment of reproductive success and courtship behaviour in a zebrafish model of autophagy-related diseases

Fontana, Camilla Maria; Locatello, Lisa; Sabatelli, Patrizia; Facchinello, Nicola; Lidron, Elisa; Maradonna, Francesca; Carnevali, Oliana; Rasotto, Maria Berica; Valle, Luisa Dalla

doi:10.1016/j.bj.2021.04.002

Cited by 5 publications

(2 citation statements)

References 28 publications

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“…Interestingly, Epg5 -specific defects in autophagy are directly linked to reduced male courtship and reproductive success in zebrafish (Fontana et al, 2021). When autophagy is interrupted in male Drosophila, mating behavior defects are observed, perhaps due to altered synaptic regulation impairing olfactory perception (Ratliff et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

A Re-Analysis of an ExistingDrosophila melanogasterDataset Reveals a New Set of Genes Involved in Post-Mating Response

Bennett,

Aramayo

2024

Preprint

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RNA sequencing (RNA-seq) is a commonly used method to identify changes in gene expression between two conditions. The analysis of RNA-seq output is complicated, with the possibility of getting different results from the same raw data. We developed and deployed four parallel pipelines to reanalyze an existing dataset of two femaleDrosophila melanogastertissue types before and after mating. TheDrosophilapost-mating response (PMR) is a well-characterized suite of changes that occur after mating, accompanied by a flux in gene expression. In comparing our study with the previous analysis of this dataset, we find our results to be more stringent, though we do identify a number of significant genes not found before. We also found variation among our own separate experiments, with gene-to-transcript isoform number and index building playing important roles in outcome. Finally, we identified a set of genes found by our pipeline that were not identified by the previous study and proposed potential roles for these genes in post-mating biology. Together, this work presents a critique of current RNA-seq analysis techniques and proposes multiple workflow adjustments that can increase the sensitivity, specificity, and stringency of differential gene expression studies.

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Section: Resultsmentioning

confidence: 99%

A Re-Analysis of an ExistingDrosophila melanogasterDataset Reveals a New Set of Genes Involved in Post-Mating Response

Bennett,

Aramayo

2024

Preprint

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“…EGP5 is a metazoan specific autophagy-related protein. The team observed a high variability in male zebrafish phenotype expression as well as an effect on their reproductive behavior, which might be linked to a lower overall reproductive success in the knockout group [ 40 ].…”

Section: Also In This Issuementioning

confidence: 99%