Eph/ephrin signaling controls cell contacts and formation of a structurally asymmetrical tissue boundary in the Xenopus gastrula

Winklbauer, Rudolf

doi:10.1016/j.ydbio.2022.07.007

Cited by 4 publications

(10 citation statements)

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“…The CM results confirm our previous findings that adhesion factors are pleiotropic, each affecting various contact types, while contact types in turn are each controlled by several adhesion factors (Barua et al 2021;Barua and Winklbauer, 2022). As an example for pleiotropic functions, HA acts together with Syn-4 and other factors in ectoderm and prechordal mesoderm to build a 50-130 nm wide glycocalyx I, and in prechordal mesoderm a micron-wide brush-like glycocalyx III (Barua et al 2021).…”

Section: Pleiotropic and Polygenic Control Of Contact Complexes By Ad...supporting

confidence: 88%

“…On the other hand, glycocalyx I is the prime example for a structure that depends on multiple adhesion factors, in the prechordal mesoderm at least on HA, Syn-4, Glypican-4, PAPC, ephrinB3 and EphB4 (Barua et al 2021;Barua and Winklbauer, 2022). The CM provides additional examples.…”

Section: Pleiotropic and Polygenic Control Of Contact Complexes By Ad...mentioning

confidence: 99%

“…For the epithelial layer, subapical adherens and tight junctions are characteristic (Mueller and Hausen, 1995;Shook and Keller, 2008;Winklbauer, 2020) while deep cell adhesive contacts appear amorphous and are interspersed between non-adhesive interstitial gaps. We recently showed that such contacts can be characterized by their width spectra -the frequency distributions of membrane-membrane distances -and by the modifications of the spectra when adhesion factors are depleted (Barua et al 2021; Barua and Winklbauer, 2022). From the requirement for factors such as fibronectin (FN) or hyaluronic acid (HA), and from the large widths of contacts we concluded that gastrula cell-cell adhesion is largely mediated by the pericellular matrix (PCM) (Winklbauer, 2019;Barua et al 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Whereas ectoderm is stretched during epiboly, prechordal mesoderm performs active, directional cell-on-cell migration (Huang and Winklbauer, 2018). Despite the differences, contact types are largely shared between these tissues (Barua et al 2021; Barua and Winklbauer, 2022). Here, we analyze cell contacts in the chordamesoderm (CM), which is continuous with the ectoderm at its posterior and the prechordal mesoderm at its anterior end.…”

Section: Introductionmentioning

confidence: 99%

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Cell contacts and pericellular matrix in the Xenopus gastrula chordamesoderm

Luu

Winklbauer

2023

Preprint

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Convergent extension of the chordamesoderm is the best-examined gastrulation movement in Xenopus. Here we study general features of cell-cell contacts in this tissue by combining depletion of adhesion factors C-cadherin, Syndecan-4, fibronectin, and hyaluronic acid, the analysis of respective contact width spectra and contact angles, and La3+ staining of the pericellular matrix. We provide evidence that like in other gastrula tissues, cell-cell adhesion in the chordamesoderm is largely mediated by different types of pericellular matrix. Specific glycocalyx structures previously identified in Xenopus gastrula tissues are absent in chordamesoderm but other contact types like 10-20 nm wide La3+ stained structures are present instead. Knockdown of any of the adhesion factors reduces the abundance of cell contacts but not the average relative adhesiveness of the remaining ones: a decrease of adhesiveness at low contact widths is compensated by an increase of contact widths and an increase of adhesiveness proportional to width. From the adhesiveness-width relationship, we derive a model of chordamesoderm cell adhesion that involves the interdigitation of distinct pericellular matrix units. Quantitative description of pericellular matrix deployment suggests that reduced contact abundance upon adhesion factor depletion is due to some contact types becoming non-adhesive and others being lost.

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Section: Pleiotropic and Polygenic Control Of Contact Complexes By Ad...supporting

confidence: 88%

Section: Pleiotropic and Polygenic Control Of Contact Complexes By Ad...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cell contacts and pericellular matrix in the Xenopus gastrula chordamesoderm

Luu

Winklbauer

2023

Preprint

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“…Whereas ectoderm is stretched during epiboly, prechordal mesoderm performs active, directional cell-on-cell migration [ 8 ]. Despite the differences, contact types are largely shared between these tissues [ 4 , 5 ]. Here, we analyze cell contacts in the chordamesoderm (CM), which is continuous with the ectoderm at its posterior and the prechordal mesoderm at its anterior end.…”

Section: Introductionmentioning

confidence: 99%

Cell contacts and pericellular matrix in the Xenopus gastrula chordamesoderm

Luu,

Barua,

Winklbauer

2024

PLoS ONE

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Convergent extension of the chordamesoderm is the best-examined gastrulation movement in Xenopus. Here we study general features of cell-cell contacts in this tissue by combining depletion of adhesion factors C-cadherin, Syndecan-4, fibronectin, and hyaluronic acid, the analysis of respective contact width spectra and contact angles, and La3+ staining of the pericellular matrix. We provide evidence that like in other gastrula tissues, cell-cell adhesion in the chordamesoderm is largely mediated by different types of pericellular matrix. Specific glycocalyx structures previously identified in Xenopus gastrula tissues are absent in chordamesoderm but other contact types like 10–20 nm wide La3+ stained structures are present instead. Knockdown of any of the adhesion factors reduces the abundance of cell contacts but not the average relative adhesiveness of the remaining ones: a decrease of adhesiveness at low contact widths is compensated by an increase of contact widths and an increase of adhesiveness proportional to width. From the adhesiveness-width relationship, we derive a model of chordamesoderm cell adhesion that involves the interdigitation of distinct pericellular matrix units. Quantitative description of pericellular matrix deployment suggests that reduced contact abundance upon adhesion factor depletion is correlated with excessive accumulation of matrix material in non-adhesive gaps and the loss of some contact types.

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