Epicardial delivery of collagen patches with adipose-derived stem cells in rat and minipig models of chronic myocardial infarction

Araña, Miriam; Gavira, Juán José; Peña, Estefanía; González, Arantxa; Abizanda, Gloria; Cilla, Myriam; Pérez, Marta; Albiasu, Edurne; Aguado, Natalia; Casado, Mayte; López, Begoña; González, Susana; Soriano, María Vicenta García; Moreno, Cristina; Merino, Juana; García‐Verdugo, José Manuel; Dı́ez, Javier; Doblaré, M.; Pelacho, Beatriz; Prósper, Felipe

doi:10.1016/j.biomaterials.2013.09.083

Cited by 97 publications

(80 citation statements)

References 37 publications

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“…Among the previously reported hydrogels, collagen have gained the greatest interest [28]. In the past years, collagen as well as collagen-containing composite materials have been widely used in the field of tissue engineering, including wound dressings, skin repair, and organ tissue engineering [29,30]. As the major component of bone, collagen was confirmed to play important roles in bone repair and new bone formation.…”

Section: Discussionmentioning

confidence: 99%

Sustained knock down of PPARγ and bFGF presentation in collagen hydrogels promote MSC osteogenesis

et al. 2015

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Collagen hydrogels were considered as favourable scaffolding for tissue engineering. It was demonstrated that cytokines and siRNAs could be efficiently retained by collagen hydrogels for controlled release thereby enhancing their bioactivities. Basic fibroblast growth factor (bFGF) was a stimulator for osteogenic differentiation of mesenchymal stem cells (MSC), and PPARγ was a key regulator in MSC osteogenic differentiation. However, whether bFGF and PPARγ could play synergetic roles within a 3D matrix to promote MSC osteogenic differentiation was unknown. In the study, bFGF and PPARγ targeting siRNAs were incorporated into collagen hydrogels for MSC cultivation. Their optimal concentrations in collagen hydrogels were determined. The capacity of bFGF/siRNA-carrying hydrogels in supporting osteogenic differentiation of MSCs was systematically evaluated with multimodality of methods, including flow cytometry, quantitative real-time PCR, Western Blotting, as well as ALP activity and calcium content determination. We demonstrated in 3D collagen hydrogel that both bFGF and siRNA molecules were efficiently retained, strengthening their effects on the incorporated MSCs. Osteogenic analysis demonstrated that the in-situ forming hydrogels carrying bFGF and siRNAs potently promoted osteogenic differentiation of incorporated MSCs, significantly superior to pure collagen and bFGF-carrying collagen. Thus, collagen hydrogels functionalized with bFGF and PPARγ targeting siRNAs may be promising in bone tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Sustained knock down of PPARγ and bFGF presentation in collagen hydrogels promote MSC osteogenesis

et al. 2015

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“…After being administered, cells can be nearly gone as soon as 1 to 3 days after transplantation [44,45]. However, some authors reported signs of stem cell survival up to 1 week [8,16,60,100,104], 1 month [14,18,23,27,30,37,46,52,67,105] and, most strikingly, up to 1 year [26]. When retained cells are quantified, we find that less than 3% of the stem cells survive more than 4 weeks [59,70] and that percentage drops to levels below 1% when the assessment is made 16 weeks after transplantation [106].…”

Section: Mechanistic Challengesmentioning

confidence: 99%

“…Like stem cells obtained from bone tissues, Adipose Tissue-derived Stem Cells (ADMSC) collection is free of ethical concerns and is associated with other pluses (Fig. 1), namely its greater handiness, the minimally invasive nature of collection, the easy and rapid in vitro expansion and the ability to differentiate into distinct lineages, such as cardiac cells [34][35][36][37][38][39][40][41]. Although broadly accepted as immune-privileged, it has been shown that ADMSCs elicit an immune response in vivo [34].…”

Section: Adipose Tissue-derived Stem Cellsmentioning

confidence: 99%

“…Abbreviations: BMMSC: bone marrow-derived mesenchymal stem cell; BMMNC: bone marrow-derived mononuclear cell; CBMSC: cortical bone-derived mesenchymal stem cell; ADMSC: adipose tissue-derived mesenchymal stem cell; PDMSC: placenta-derived mesenchymal stem cell; PDAC: placenta-derived adherent cell; AFPC: amniotic fluid-derived progenitor cell; CL-MSC: subamnion-cord-lining mesenchymal stem cell; UCB-EPC: umbilical cord blood-derived endothelial progenitor cell; UCB-MNC: umbilical cord blood-derived mononuclear cell; CP-MSC: chorionic plate-derived mesenchymal stem cell; fC-MSC: fetal cardiac mesenchymal stem cell; CSC: cardiac stem cell; CMPC: cardiomyocyte progenitor cell; SkMSC: skeletal muscle stem cell; iPSC: induced pluripotent stem cell. paracrine means and encompasses angiogenesis stimulation, cell death arrest, regulation of fibrosis, but also by direct cardiomyogenesis [8,37,41].…”

Section: Adipose Tissue-derived Stem Cellsmentioning

confidence: 99%

“…The advantages and limitations of each stem cell with regard to the possible emergence of ethical concerns (symbolized by ¥), the invasiveness and collection (from + to ++++), the isolation procedure, the abundance (from + to ++++), expansion and proliferation rate, the transdifferentiation capacity, the paracrine effects or/and cardiomyogenic potential, the survival and engraftment capacity as well as the immunogenic, oncogenic or teratogenic risks is given in the accessory side tables. The left table abridges bone marrow-derived stem cells [3,[19][20][21]27,28], cortical bone-derived stem cells [32,33], adipose tissue-derived stem cells [34,37,39,41] and gestation-related stem cells [42][43][44][45][46][47][48][49]51,54] and the right table abridges cardiac stem cells [57,59,60], skeletal muscle stem cells [62,63,66] and induced pluripotent stem cells [67,[69][70][71]. The human body scheme was constructed with images sourced from Servier Medical Art (http://www.servier.com/ Powerpoint-image-bank) and Cientic website (http://www.cientic.com/).…”

Section: Adipose Tissue-derived Stem Cellsmentioning

confidence: 99%

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Towards the standardization of stem cell therapy studies for ischemic heart diseases: Bridging the gap between animal models and the clinical setting

Trindade

Leite‐Moreira

Ferreira-Martins

et al. 2017

International Journal of Cardiology

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Today there is an increasing demand for heart transplantations for patients diagnosed with heart failure. Though, shortage of donors as well as the large number of ineligible patients hurdle such treatment option. This, in addition to the considerable number of transplant rejections, has driven the clinical research towards the field of regenerative medicine. Nonetheless, to date, several stem cell therapies tested in animal models fall by the wayside and when they meet the criteria to clinical trials, subjects often exhibit modest improvements. A main issue slowing down the admission of such therapies in the domain of human trials is the lack of protocol standardization between research groups, which hampers comparison between different approaches as well as the lack of thought regarding the clinical translation. In this sense, given the large amount of reports on stem cell therapy studies in animal models reported in the last 3 years, we sought to evaluate their advantages and limitations towards the clinical setting and provide some suggestions for the forthcoming investigations. We expect, with this review, to start a new paradigm on regenerative medicine, by evoking the debate on how to plan novel stem cell therapy studies with animal models in order to achieve more consistent scientific production and accelerate the admission of stem cell therapies in the clinical setting.

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Tissue engineering

and

Seifalian

2015

Plastic and Reconstructive Surgery

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Epicardial delivery of collagen patches with adipose-derived stem cells in rat and minipig models of chronic myocardial infarction

Cited by 97 publications

References 37 publications

Sustained knock down of PPARγ and bFGF presentation in collagen hydrogels promote MSC osteogenesis

Sustained knock down of PPARγ and bFGF presentation in collagen hydrogels promote MSC osteogenesis

Towards the standardization of stem cell therapy studies for ischemic heart diseases: Bridging the gap between animal models and the clinical setting

Tissue engineering

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