Epicatechin-3-O-(3″-O-methyl)-gallate Content in Various Tea Cultivars (Camellia sinensis L.) and Its in Vitro Inhibitory Effect on Histamine Release

Maeda‐Yamamoto, Mari; Ema, Kaori; Monobe, Mamami; Tokuda, Yoshiko; Tachibana, Hirofumi

doi:10.1021/jf204497b

Cited by 64 publications

(42 citation statements)

References 30 publications

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“…In addition, an increased anti-allergic effect has been reported for methylated EGCG and other catechins relative to the unmethylated forms [216]. On the other hand, other studies have reported a decrease in the antioxidant activity of methylated catechins [217].…”

Section: Effects On Ttr Toxicity In Vivomentioning

confidence: 99%

Modulators of amyloid protein aggregation and toxicity: EGCG and CLR01

Attar

Rahimi

Bitan

2013

Translational Neuroscience

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Abnormal protein folding and self-assembly causes over 30 cureless human diseases for which no diseasemodifying therapies are available. The common side to all these diseases is formation of aberrant toxic protein oligomers and amyloid fibrils. Both types of assemblies are drug targets, yet each presents major challenges to drug design, discovery, and development. In this review, we focus on two small molecules that inhibit formation of toxic amyloid protein assemblies -the green-tea derivative (-)-epigallocatechin-3-gallate (EGCG), which was identified through a combination of epidemiologic data and a compound library screen, and the molecular tweezer CLR01, whose inhibitory activity was discovered in our group based on rational reasoning, and subsequently confirmed experimentally. Both compounds act in a manner that is not specific to one particular protein and thus are useful against a multitude of amyloidogenic proteins, yet they act via distinct putative mechanisms. CLR01 disrupts protein aggregation through specific binding to lysine residues, whereas the mechanisms underlying the activity of EGCG are only recently beginning to unveil. We discuss current in vitro and, where available, in vivo literature related to EGCG and CLR01's effects on amyloid β-protein, α-synuclein, transthyretin, islet amyloid polypeptide, and calcitonin. We also describe the toxicity, pharmacokinetics, and mechanism of action of each compound.

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Section: Effects On Ttr Toxicity In Vivomentioning

confidence: 99%

Modulators of amyloid protein aggregation and toxicity: EGCG and CLR01

Attar

Rahimi

Bitan

2013

Translational Neuroscience

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“…Previous studies have demonstrated that one consumption of green tea extract 1.5-4.5 g or 1-3 cups by healthy individuals results in plasma concentrations of EGCG of only 4 µM 29 . The ingestion of benifuuki tea, which contains O-methylated catechin, results in higher EGCG blood concentrations and may have a more bene cial effect than other green teas 30 . It is necessary to devise an effective means to administer EGCG, such as O-methylated EGCG, because it will be dif cult to immediately use EGCG in a clinical setting.…”

Section: Discussionmentioning

confidence: 99%

Quantification of (–)-Epigallocatechin-3-gallate Inhibition of Anaplastic Thyroid Cancer Cell Line Adhesion and Proliferation Using Real-time Cell Analysis

昌行

Miyazawa

2016

Showa Univ J Med Sci

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: Anaplastic thyroid cancer ATC has a poor prognosis because of immediate metastasis. Several studies in humans and animals have suggested that the ingestion of green tea or its active ingredient --epigallocatechin-3-gallate EGCG may decrease the risk of cancer. Using a recently developed real-time cell analysis RTCA system, we have shown previously that EGCG inhibits cell migration and the invasion of oral cavity cancers by suppressing matrix metalloproteinases. In the present study we used RTCA to investigate the effects of EGCG on cell adhesion to bronectin-coated plates using three cancer cell lines : one ATC cell line TCO-1 and two poorly differentiated oral squamous cell carcinomas OSCCs cell lines SAS and HO-1-u-1 ; originating from the tongue and oor of the mouth, respectively . EGCG 50 µM inhibited the adhesion of all three cell lines. In addition to its effects on cell adhesion, 50 µM EGCG inhibited the cell proliferation of TCO-1 cells. Furthermore, EGCG decreased V integrin ITGAV mRNA levels in all three cell lines, suggesting that EGCG inhibits the cell adhesion and proliferation of OSCC and ATC cells via suppression of integrin expression. Therefore, EGCG represents a useful dietary constituent or a lead compound for counteracting metastasis of oral cavity cancers and thyroid cancers.

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“…With regard to anthocyanins, four delphinidin and four cyaniding glycosides were determined by high-performance liquid chromatography (HPLC), as described in our previous report (15). In addition to these ingredients, catechin, caffeine and amino acid levels in the tea samples were also measured by HPLC, as described in previous reports (16,17). The contents of flavonol glycosides, catechins, anthocyanins, caffeine and amino acids in each green tea sample are shown in Table 1.…”

Section: Green Tea Samplesmentioning

confidence: 99%

Comparison of the Effects of Three Tea Cultivars (Camellia sinensis L.) on Nitric Oxide Production and Aortic Soluble Guanylate Cyclase Expression in High-Salt Diet-Fed Spontaneously Hypertensive Rats

Nomura

Monobe

Ema

et al. 2017

J Nutr Sci Vitaminol

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Summary Studies have suggested that the consumption of green tea reduces the risk of cardiovascular diseases. Although epigallocatechin gallate (EGCG) is the best studied active substance characteristic of green tea, previous results on EGCG do not appear sufficient to explain completely the mechanism of cardiovascular protection by green tea. Therefore, we investigated the effect of three different tea cultivars, "Yabukita," "Sofu," and "Sunrouge," which have characteristic flavonoid compositions, on the nitric oxide (NO) production and the related protein expression in the aorta of spontaneously hypertensive rats (SHRs) fed a high-salt diet. As a result, the reduction of urinary NO metabolite (NOx) levels, which reflect whole-body NO production, caused by the high-salt diet were significantly prevented by all three tea infusions. The improvement of NOx reduction in the tea-intake groups was unlikely to be caused by the changes in oxidative damage. On the other hand, as a partial effect, only "Yabukita" or "Sofu" increased the expression of the soluble guanylate cyclase, a receptor for NO, in the thoracic aorta. In the present study, the differences in the composition of these three cultivars led to partially different effects on NO signaling in SHRs, suggesting the physiological significance of subdominant ingredients besides EGCG.

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Epicatechin-3-O-(3″-O-methyl)-gallate Content in Various Tea Cultivars (Camellia sinensis L.) and Its in Vitro Inhibitory Effect on Histamine Release

Cited by 64 publications

References 30 publications

Modulators of amyloid protein aggregation and toxicity: EGCG and CLR01

Modulators of amyloid protein aggregation and toxicity: EGCG and CLR01

Quantification of (–)-Epigallocatechin-3-gallate Inhibition of Anaplastic Thyroid Cancer Cell Line Adhesion and Proliferation Using Real-time Cell Analysis

Comparison of the Effects of Three Tea Cultivars (<i>Camellia sinensis</i> L.) on Nitric Oxide Production and Aortic Soluble Guanylate Cyclase Expression in High-Salt Diet-Fed Spontaneously Hypertensive Rats

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Epicatechin-3-O-(3″-O-methyl)-gallate Content in Various Tea Cultivars (Camellia sinensis L.) and Its in Vitro Inhibitory Effect on Histamine Release

Cited by 64 publications

References 30 publications

Modulators of amyloid protein aggregation and toxicity: EGCG and CLR01

Modulators of amyloid protein aggregation and toxicity: EGCG and CLR01

Quantification of (&ndash;)-Epigallocatechin-3-gallate Inhibition of Anaplastic Thyroid Cancer Cell Line Adhesion and Proliferation Using Real-time Cell Analysis

Comparison of the Effects of Three Tea Cultivars (<i>Camellia sinensis</i> L.) on Nitric Oxide Production and Aortic Soluble Guanylate Cyclase Expression in High-Salt Diet-Fed Spontaneously Hypertensive Rats

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Quantification of (–)-Epigallocatechin-3-gallate Inhibition of Anaplastic Thyroid Cancer Cell Line Adhesion and Proliferation Using Real-time Cell Analysis