Epicatechin protective effects on bleomycin-induced pulmonary oxidative stress and fibrosis in mice

Shariati, Saeedeh; Kalantar, Hadi; Pashmforoosh, Marzieh; Mansouri, Esrafil; Khodayar, Mohammad Javad

doi:10.1016/j.biopha.2019.108776

Cited by 53 publications

(30 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on the classification in previous experiments, the process of pulmonary fibrosis is divided into two stages. The early stage is the inflammatory phase, at around 0-7 days after bleomycin administration, while the later stage is the fibrotic phase, starting from 7 days of bleomycin administration [26]. According to these information, we checked the severity of pulmonary fibrosis at day 14 but explored the effects of PM on inflammatory phases by specifically evaluating the changes in immune cell number and profile in BALF between days 2 and 7.…”

Section: Discussionmentioning

confidence: 99%

Particulate Matter Increases the Severity of Bleomycin-Induced Pulmonary Fibrosis through KC-Mediated Neutrophil Chemotaxis

Cheng¹,

Liu

Lin

et al. 2019

IJMS

View full text Add to dashboard Cite

Background: Although particular matter (PM) increases incidence and severity of idiopathic pulmonary fibrosis, the underlying mechanism remains elusive. Methods: The effects of PM were evaluated in a murine model of bleomycin-induced pulmonary fibrosis. Mice were divided into four groups, receiving: (1) Saline (control), (2) bleomycin, (3) PM, or (4) bleomycin plus PM (Bleo+PM). Additional groups of Bleo+PM mice were treated with sivelestat (an inhibitor of neutrophil elastase) or reparixin (a C-X-C motif chemokine receptor 2 antagonist), or were genetically modified with keratinocyte chemoattractant (KC) deletion. Results: Pulmonary fibrosis was not observed in the control or PM groups. Bleomycin induced pulmonary fibrosis within 14 days. The Bleo+PM group showed worse pulmonary fibrosis when compared to the bleomycin group. Analyses of immune cell profile and chemokine/cytokine concentrations at day 2-bronchoalveolar lavage fluid (BALF) revealed that the Bleo+PM group had increased neutrophil number and elastase level and KC concentration compared to the bleomycin group. Neutrophil elastase activated the Smad2/Smad3/α-SMA pathway to induce collagen deposition, while sivelestat abrogated the increased severity of pulmonary fibrosis caused by PM. Chemotaxis assay revealed that BALF of the Bleo+PM group recruited neutrophil, which was dependent on KC. Further, genetic KC deletion or pharmaceutical inhibition of KC binding to CXCR2 with reparixin ameliorated the PM-induced increased severity of pulmonary fibrosis. Conclusions: These data provide evidence that the PM-induced increased severity of pulmonary fibrosis depends on KC-mediated neutrophil chemotaxis and give additional mechanic insight that will aid in the development of therapeutic strategies.

show abstract

Section: Discussionmentioning

confidence: 99%

Particulate Matter Increases the Severity of Bleomycin-Induced Pulmonary Fibrosis through KC-Mediated Neutrophil Chemotaxis

Cheng¹,

Liu

Lin

et al. 2019

IJMS

View full text Add to dashboard Cite

show abstract

“…The mechanism of BML-induced lung injury is not entirely clear, but likely includes components of oxidative damage, relative deficiency of the deactivating enzyme BML hydrolase, genetic susceptibility, and elaboration of inflammatory cytokines. Oxidative damage to the lung appears important in the pathophysiology of lung injury, and antioxidants may ameliorate the process [19]. Systemic administration of antioxidant artemisitene strongly inhibits bleomycin-induced lung damage, through the activation of the Nrf2 signaling pathway [20].…”

Section: Bleomycin-induced Toxicitymentioning

confidence: 99%

Modulation of Oxidative Stress by Ozone Therapy in the Prevention and Treatment of Chemotherapy-Induced Toxicity: Review and Prospects

Clavo

Rodrı́guez-Esparragón

Rodríguez-Abreu

et al. 2019

Antioxidants

View full text Add to dashboard Cite

(1) Background: Cancer is one of the leading causes of mortality worldwide. Radiotherapy and chemotherapy attempt to kill tumor cells by different mechanisms mediated by an intracellular increase of free radicals. However, free radicals can also increase in healthy cells and lead to oxidative stress, resulting in further damage to healthy tissues. Approaches to prevent or treat many of these side effects are limited. Ozone therapy can induce a controlled oxidative stress able to stimulate an adaptive antioxidant response in healthy tissue. This review describes the studies using ozone therapy to prevent and/or treat chemotherapy-induced toxicity, and how its effect is linked to a modification of free radicals and antioxidants. (2) Methods: This review encompasses a total of 13 peer-reviewed original articles (most of them with assessment of oxidative stress parameters) and some related works. It is mainly focused on four drugs: Cisplatin, Methotrexate, Doxorubicin, and Bleomycin. (3) Results: In experimental models and the few existing clinical studies, modulation of free radicals and antioxidants by ozone therapy was associated with decreased chemotherapy-induced toxicity. (4) Conclusions: The potential role of ozone therapy in the management of chemotherapy-induced toxicity merits further research. Randomized controlled trials are ongoing.

show abstract

“…However, different exposures might cause lung fibrosis through distinct mechanisms. Small molecules, such as bleomycin, might induce fibrosis by primarily inducing tissue damage through metabolic stress [ 32 ]. This, consequently, activates the immune system as a secondary event.…”

mentioning

confidence: 99%

Covid-19 acute responses and possible long term consequences: What nanotoxicology can teach us

2020

View full text Add to dashboard Cite

show abstract

Epicatechin protective effects on bleomycin-induced pulmonary oxidative stress and fibrosis in mice

Cited by 53 publications

References 54 publications

Particulate Matter Increases the Severity of Bleomycin-Induced Pulmonary Fibrosis through KC-Mediated Neutrophil Chemotaxis

Particulate Matter Increases the Severity of Bleomycin-Induced Pulmonary Fibrosis through KC-Mediated Neutrophil Chemotaxis

Modulation of Oxidative Stress by Ozone Therapy in the Prevention and Treatment of Chemotherapy-Induced Toxicity: Review and Prospects

Covid-19 acute responses and possible long term consequences: What nanotoxicology can teach us

Contact Info

Product

Resources

About