Epidemic of Surgical-Site Infections By a Single Clone of Rapidly Growing Mycobacteria in Brazil

Leão, Sylvia Cardoso; Viana-Niero, Cristina; Matsumoto, Cristianne Kayoko; Lima, Karla Valéria Batista; Lopes, Maria Luíza; Palaci, Moisés; Hadad, David Jamil; Vinhas, Solange Alves; Duarte, Rafael Silva; Lourenço, Maria Cristina S.; Kipnis, André; Neves, Zilah Cândida das; Gabardo, Betina Mendez Alcântara; Ribeiro, Marta Osório; Baethgen, Ludmila Fiorenzano; Assis, Denise Brandão de; Madalosso, Geraldine; Chimara, Érica; Dalcolmo, Margareth Pretti

doi:10.2217/fmb.10.49

Cited by 82 publications

(85 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…All of the obtained sequences were highly similar to the rpoB sequence of Mycobacterium massiliense CCUG 48898 (GenBank accession AY593981.2) and had two base substitutions (GenBank accession EU117207) (Leão et al 2010). A total of 152 isolates obtained from surgical patients in different states belonged to a single strain and showed two closely related pulsed field gel electrophoresis (PFGE) patterns with a one-band difference when using either DraI or AseI restriction enzymes (Cardoso et al 2008, Duarte et al 2009, Leão et al 2010. This strain was classified as M. abscessus subsp.…”

mentioning

confidence: 76%

“…Isolates were classified as belonging to the epidemic strain when they showed one of the two PFGE patterns that were previously defined as being characteristic of this strain (Leão et al 2010).…”

Section: Subjects Materials and Methodsmentioning

confidence: 99%

“…Only one isolate per patient was included. These isolates had been previously characterised by rpoB gene sequencing, PFGE-DraI and PFGE-AseI , da Costa et al 2009, Duarte et al 2009, Leão et al 2010, Matsumoto et al 2011.…”

Section: Subjects Materials and Methodsmentioning

confidence: 99%

“…PFGE typing -PFGE typing was performed as described in previous publications from our group (Leão et al 2010, Matsumoto et al 2011. PFGE patterns were analysed using the BioNumerics v. 5.1 program (Applied Maths, Sint-Martens-Laten, Belgium).…”

Section: Subjects Materials and Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Rapid tests for the detection of the Mycobacterium abscessus subsp. bolletii strain responsible for an epidemic of surgical-site infections in Brazil

Matsumoto

Chimara

Ramos

et al. 2012

Mem. Inst. Oswaldo Cruz

Self Cite

View full text Add to dashboard Cite

mentioning

confidence: 76%

Section: Subjects Materials and Methodsmentioning

confidence: 99%

Section: Subjects Materials and Methodsmentioning

confidence: 99%

Section: Subjects Materials and Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Rapid tests for the detection of the Mycobacterium abscessus subsp. bolletii strain responsible for an epidemic of surgical-site infections in Brazil

Matsumoto

Chimara

Ramos

et al. 2012

Mem. Inst. Oswaldo Cruz

Self Cite

View full text Add to dashboard Cite

“…bolletii (M. bolletii)-comprise the so-called "M. abscessus group" (MAG) (1)(2)(3). MAG is commonly associated with chronic lung infections in susceptible hosts, such as cystic fibrosis (CF) (4), as well as wound infection and postsurgical site infections (5,6). Macrolides such as clarithromycin and azithromycin are frequently the only oral antibiotics that are active against MAG and are commonly used to treat pulmonary infections (7,8).…”

mentioning

confidence: 99%

New Real-Time PCR Assays for Detection of Inducible and Acquired Clarithromycin Resistance in the Mycobacterium abscessus Group

et al. 2015

View full text Add to dashboard Cite

Members of the. MAG is commonly associated with chronic lung infections in susceptible hosts, such as cystic fibrosis (CF) (4), as well as wound infection and postsurgical site infections (5, 6). Macrolides such as clarithromycin and azithromycin are frequently the only oral antibiotics that are active against MAG and are commonly used to treat pulmonary infections (7,8). Members of the MAG differ in their susceptibility to clarithromycin. M. abscessus and M. bolletii, commonly display inducible macrolide resistance conferred by the ribosomal methyl transferase erm(41) gene (9). In contrast, most M. massiliense strains do not show inducible resistance due to a large, 274-bp deletion in the ribosomal methyl transferase erm(41) gene that renders it nonfunctional (9, 10), and clinical studies have shown a better response to clarithromycin in M. massiliense compared to M. abscessus (8, 10). Besides this major truncation, erm(41) can lose functionality by a T-to-C substitution at position 28 (T28C) yielding a Trp-to-Arg amino acid change (9, 11). The second mechanism of clarithromycin resistance is acquired through mutations in the drug-binding pocket of the 23S rRNA rrl gene at nucleotide positions 2058 and 2059 (12-15, 27). Phenotypic detection of clarithromycin resistance requires incubation of MAG with clarithromycin for up to 14 days. While MAG strains displaying acquired resistance show high MICs to clarithromycin after 3 to 5 days, those with an inducible active erm(41) gene typically show low MICs at 3 to 5 days and require longer incubation times (up to 14 days) for induction of resistance (16).Targeted sequencing is routinely used in the clinical laboratory to determine the intraspecies genetic diversity in mycobacterial isolates and discriminate among closely related taxa. Multiple genes, including rpoB, hsp65, secA,, as well as PCR-based assays (10, 23) have been evaluated as tools to discriminate between the closely related subspecies of the MAG. Since a truncated erm(41) gene was described as a hallmark of M. massiliense, size differences in PCR-amplified erm(41) PCR products were proposed as a simple method to differentiate M. massiliense from M. abscessus and M. bolletii (10). However, this method can misclassify some strains, such as two recently described M. massiliense strains harboring a full-length and functional erm(41) gene (23).Since standard susceptibility testing of clarithromycin requires up to 14 days, there is a need for faster methods to detect clari-

show abstract

Mycobacterium : Clinical and Laboratory Characteristics of Rapidly Growing Mycobacteria

Brown‐Elliott,

Harrington,

Morimoto

et al. 2023

ClinMicroNow

View full text Add to dashboard Cite

Rapidly growing mycobacteria (RGM) contain long‐chain fatty acids, known as mycolic acids, that can be quantitated with chromatographic techniques such as high‐performance liquid chromatography (HPLC). Prior to the molecular era, HPLC was used for identification of RGM species in most major reference laboratories. The RGM are opportunistic pathogens that cause disease in a variety of clinical settings. RGM may also cause bone and joint infections. Chronic lung infections with RGM, most often in nonsmoking older women with bronchiectasis, are sometimes associated with the Mycobacterium avium complex. The use of mycobacterial smears is a rapid and reasonably sensitive step in the diagnosis of RGM disease. PCR amplification and sequencing has been used to detect and identify nontuberculous mycobacteria in specimens from sterile sites, but these tests are generally limited to larger, reference laboratories. The adjunctive nonmolecular tests, including antimicrobial susceptibility tests, have been utilized for preliminary identification of the RGM.

show abstract

Epidemic of Surgical-Site Infections By a Single Clone of Rapidly Growing Mycobacteria in Brazil

Abstract: A particular strain of M. abscessus ssp. massiliense was associated with a prolonged epidemic of postsurgical infections in seven Brazilian states, suggesting that this strain may be distributed in Brazilian territory and better adapted to cause surgical-site infections.

Cited by 82 publications

References 24 publications

Rapid tests for the detection of the Mycobacterium abscessus subsp. bolletii strain responsible for an epidemic of surgical-site infections in Brazil

Rapid tests for the detection of the Mycobacterium abscessus subsp. bolletii strain responsible for an epidemic of surgical-site infections in Brazil

New Real-Time PCR Assays for Detection of Inducible and Acquired Clarithromycin Resistance in the Mycobacterium abscessus Group

Mycobacterium : Clinical and Laboratory Characteristics of Rapidly Growing Mycobacteria

Contact Info

Product

Resources

About