Epidemic Potential of Escherichia coli O16:H41-ST131: Compared with Pandemic O25b:H30-ST131 Lineage

Zhang, Shengcen; Zhang, Qianwen; Huang, Jiyue; Cao, Yong; Zhao, Zhichang; Li, Bin

doi:10.2147/idr.s313261

Cited by 4 publications

(5 citation statements)

References 26 publications

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“…O25b has traditionally been considered the predominant serotype of ST131, but the prevalence of E. coli O16-ST131 has increased significantly in recent years. 49 In our study, the detection rate of O16-ST131 was higher than that of O25b-ST131, which was consistent with our past results of a study on healthy adults in Changsha. 34 The rise of O16-ST131 may be related to the improvement of novel PCR detection methods.…”

Section: Discussionsupporting

confidence: 92%

Community Fecal Carriage and Molecular Epidemiology of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Escherichia coli from Healthy Children in the Central South China

Yang

et al. 2022

IDR

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Background Fecal carriage of extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC) and carbapenemase-producing E. coli (CP-EC) is well reported among hospitalized adults and children. However, there are few studies on the carriage prevalence and ESBL-EC and CP-EC genotypes among healthy children in China. Patients and Methods Stool samples were collected from 330 students in 2021 from three randomly selected primary schools in Changsha, China. ESBL-EC and CP-EC were screened using CHROMagar TM chromogenic plates. ESBL and carbapenemase production was confirmed using the double-disc synergy test and a modified carbapenem inactivation method, respectively. Antimicrobial susceptibility was tested using the broth microdilution method. Resistance determinants, virulence factors, and phylogenetic groups were determined by PCR and sequencing. Multi-locus sequence typing (MLST) was performed (seven housekeeping genes were amplified and sequenced) on the phylogenic group B2 E. coli to detect high-risk clonal strains such as ST131 E. coli . Then, ST131 E. coli were characterized based on ST131 clades, O-type, and fimH alleles. Results In total, 118 (35.8%) ESBL-EC and 3 (0.9%) CP-EC were isolated. bla CTX-M was the most common genotype (27.1%), identified in all ESBL-EC, except one, which carried bla SHV-12 . One isolate with mcr-1 was found amongst ESBL-EC, whereas all three CP-EC carried bla NDM-1 . The predominant sequence type (ST) clones in group B2 were ST131 and ST1193. The prevalence of ST131 E. coli was 9.9%, displaying serotypes O16 and O25b, fimH alleles 30, 41, and 89, and ST131 clades A and C1-M27. Conclusion In this study, high carriage rate of ESBL-EC was found among healthy children, and the dominant ESBL was CTX-M-14. In addition, high-risk clones (ST131 and ST1193) were also detected. This emphasizes the importance of monitoring ESBL-EC in community settings.

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Section: Discussionsupporting

confidence: 92%

Community Fecal Carriage and Molecular Epidemiology of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Escherichia coli from Healthy Children in the Central South China

Yang

et al. 2022

IDR

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“…We examined the biofilm formation ability among all strains and found that E. coli O25b/ST131 isolates were much superior in this capability, according to Kudinha et al's study [35]. In contrast, Zhang et al reported most E. coli ST131 isolates could produce weak biofilms [36]. Sarkar et al revealed that type I fimbriae play a vital role in biofilm formation and inhibition of FimH adhesion, which significantly reduces biofilm production [37].…”

Section: Discussionmentioning

confidence: 95%

Distribution and expression of virulence genes (hlyA, sat) and genotyping of Escherichia coli O25b/ST131 by multi-locus variable number tandem repeat analysis in Tehran, Iran

Asgharzadeh

Zadeh

Moghadam

et al. 2022

AMicr

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Escherichia coli ST131 is a pandemic clone with high antibiotic resistance, and it is a major causative agent of urinary tract infection (UTI) and bloodstream infections. This study evaluated the distribution and expression of virulence genes and genotyping of E. coli O25b/ST131 by Multi-locus variable number tandem repeat analysis (MLVA) method among UTI in patients at Tehran hospitals, Iran. A total of 107 E. coli isolates were collected from UTI patients. Polymerase chain reaction (PCR) amplification of the pabB gene was used to identify E. coli O25b/ST131 and the prevalence of sat and hlyA virulence genes was also analyzed. The microtiter method quantified biofilm formation ability in E. coli O25b/ST131. The Real-Time PCR (qRT-PCR) was performed to evaluate the expression of sat and hlyA genes. Finally, MLVA was performed for E. coli O25b/ST131 genotyping by targeting seven tandem repeats. SPSS-16 software was used for statistical analysis. Molecular study showed that 71% of isolates carried the pabB gene and were considered E. coli O25b/ST131 strains. Also, 45.8% and 17.8% of isolates carried sat and hlyA genes, respectively. The 57.9% isolates had biofilm formation ability. Expression of the studied virulence genes showed an increase in strong biofilm producing E. coli O25b/ST131 strains. A total of 76 (100%) E. coli O25b/ST131 strains were typed by the MLVA method. High prevalence of E. coli O25b/ST131 isolates in UTI patients can be a serious warning to the treatment due to the high antibiotic resistance rate, expression of virulence genes, and biofilm formation.

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“…coli ST131 strains formed biofilm, and they reported that the ability of ST131 strains to form biofilm was significantly higher compared to non-ST131 strains [ 17 ]. A study conducted in China found that 70% of E. coli ST131 strains formed biofilm [ 18 ]. In our study, it was found that 69.7% of the samples that were positive for ST131 clone formed biofilm by any method, while 30.3% did not form biofilm.…”

Section: Discussionmentioning

confidence: 99%

“…Few studies have investigated the biofilm production of E. coli ST131 isolates. These studies have reported high rates of biofilm production by ST131 strains [ 16 – 18 ]. They reported that this clone exhibits a specific O25b molecular subtype and produces biofilms, making it highly virulent, although it lacks the classic extraintestinal pathogenicity islands and afa/dra gene [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of presence of clone ST131 and biofilm formation in ESBL producing and non-producing Escherichia coli strains

Çelebi

Aydın²,

Rakici

et al. 2023

Mol Biol Rep

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Objective Escherichia coli ST131 is a pandemic clone associated with multidrug resistance, starting with beta-lactamase production and fluoroquinolone resistance in the first place, leading to significant systemic infections. Clones that develop due to the frequency of antimicrobial resistance and the rate of spread in our country are important issues that need to be investigated. This study aims to investigate the incidence of ST131which is a “high-risk pandemic clone E. coli ” in ESBL-producing and non-ESBL-producing strains, as well as their biofilm-forming abilities and antibiotic resistance rates. Materials and methods A total of 86 E. coli isolates were used in the study. Bacterial identifications were performed by conventional and automated methods. The double disc synergy method was used to demonstrate the presence of ESBL. Molecular studies in all E. coli strains were performed by real-time PCR method. Findings: 86 strains were studied, of which 83.72% were urine, 6.98% were wound, 4.65% were blood, and 2.33% were tracheal aspirate and sputum. 79.07% of these strains were ESBL-positive. 58.1% of the strains were female, whereas 41.9% were male patients, and the average age was 46.2. Out of 86 strains, 38.72% were ST131 positive, the H30 subclone was detected in 27.27% of them, and the H30-Rx subclone was detected in all of the H30 subclone positive strains. The presence of the ESBL resistance gene was detected at the rate of TEM 41.86%, SHV 37.21%, CTX-M 36.04%, and OXA 4.65%. Most commonly SHV gene (54.54%) was seen in ST131 clone-positive samples. Finally, while it was found that 48.83% of the strains formed biofilm by any method, biofilm formation was detected in 69.7% of the samples that were positive for the ST131 clone. Result Our study can reveal the dramatic prevalence of the ESBL-producing E. coli strains along with the high-risk ST131 clone, the dominance of the H30Rx subclone of this risky clone, as well as the importance of the influence of resistance mechanisms along with resistance and biofilm.

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Epidemic Potential of Escherichia coli O16:H41-ST131: Compared with Pandemic O25b:H30-ST131 Lineage

Cited by 4 publications

References 26 publications

Community Fecal Carriage and Molecular Epidemiology of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Escherichia coli from Healthy Children in the Central South China

Community Fecal Carriage and Molecular Epidemiology of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Escherichia coli from Healthy Children in the Central South China

Distribution and expression of virulence genes (hlyA, sat) and genotyping of Escherichia coli O25b/ST131 by multi-locus variable number tandem repeat analysis in Tehran, Iran

Evaluation of presence of clone ST131 and biofilm formation in ESBL producing and non-producing Escherichia coli strains

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