Epidemiological and genetic characteristics of porcine reproductive and respiratory syndrome virus circulating in central and South China in 2016

Liang, Wan; Zhao, Tingting; Peng, Zhong; Sun, Yinyan; Stratton, Charles W.; Zhou, Dana; Tang, Xibiao; Tian, Yao; Chen, Huanchun; Wu, Bin

doi:10.1016/j.actatropica.2018.11.004

Cited by 17 publications

(16 citation statements)

References 31 publications

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“…In this study, the HP-PRRSV strain accounted for the highest proportion of epidemic strains in China; the NADC30-like strain had increased gradually, which was in accordance with the results of Gao et al (23). However, some studies have shown that the NADC30-like strain in some regions of China have replaced the HP-PRRSV strain, and has become a new dominant strain (22). The rising infection rate of HP-PRRSV and NADC30-like strains may lead to a significant decrease in the effective protection rate of vaccines on pig farms.…”

Section: Discussionsupporting

confidence: 91%

“…In previous studies, the positive rate of PRRSV was shown to be 55.21% (7,490/11,3567) in 29 provinces of China in 2012-2015 (21). In Central and Southern China, there was a positive rate of 50.62% (530/1,047) of PRRSV among 257 pig farms (22). In our study, the total positive rate of PRRSV was 50.89% (57/112) in nine provinces of China from 2016 to 2018, which was in accordance with the above scholars.…”

Section: Discussionmentioning

confidence: 90%

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Molecular Characterization of the Nsp2 and ORF5 (ORF5a) Genes of PRRSV Strains in Nine Provinces of China During 2016–2018

Yin

Wang

et al. 2021

Front. Vet. Sci.

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Porcine reproductive and respiratory syndrome virus (PRRSV) causes a highly contagious disease and brings huge economic losses to commercial pork production worldwide. PRRSV causes severe reproductive failure in sows and respiratory distress in piglets. To trace the evolution of PRRSV in pigs with respiratory diseases in some regions of China, 112 samples were collected from nine provinces in China during 2016–2018. All samples were detected by RT-PCR and analyzed by the Nsp2/ORF5 (ORF5a)-genes-phylogeny. Sequence analysis and recombination analysis were conducted on the Nsp2/ORF5 (ORF5a) genes of the identified strain in the study. The RT-PCR result shown that the positive rate of PRRSV was 50.89% (57/112). Phylogenetic analysis showed that the identified PRRSV strains were all NA genotype and belonged to lineage 1, 3, and 8. The Nsp2 gene of identified PRRSV strains exhibited nucleotide homologies of 53.0 ~ 99.8%, and amino acid homologies of 46.8 ~ 99.7%. The ORF5 gene of identified PRRSV strains exhibited nucleotide homologies of 82.4 ~ 100%, and amino acid homologies of 79.6 ~ 100%. Sequence analysis revealed that a discontinuous 30-amino-acid deletion (positions 481 and 533–561) and a 131-amino-acid discontinuity deletion (positions 323–433, 481, and 533–551) in Nsp2 of PPRSV isolates; all identified strains in this study may be wild strains, and most identified strains may be highly virulent strains. Sequence analysis of ORF5 and ORF5a revealed that the mutation sites of GP5 were mainly concentrated in the signal peptide and epitopes region, while the mutation sites of ORF5a were mainly concentrated in the transmembrane and the intramembrane region. The recombination analysis indicated that there may be multiple recombination regions in identified strains, and the recombination pattern was more complex. This study showed that the prevalent PRRSV strain in some regions of China was still HP-PRRSV, while NADC30 strain also occupied a certain proportion; different types of PRRSV strains showed different patterns and variation in China. This study suggested that the monitoring of PRRSV prevalence and genetic variation should be further strengthened.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 90%

Molecular Characterization of the Nsp2 and ORF5 (ORF5a) Genes of PRRSV Strains in Nine Provinces of China During 2016–2018

Yin

Wang

et al. 2021

Front. Vet. Sci.

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“…As shown in this review, the emergence of new strains such as NADC30-like, represents a high mutation stage of the PRRSV epidemic in China, and PRRSV-2 appears to become more common ( 106 , 107 ). Not only the NADC30-like strain itself is highly recombinant, but its coinfection with other pathogens increased the mutation rate of PRRSV.…”

Section: Perspectivesmentioning

confidence: 90%

Advanced Research in Porcine Reproductive and Respiratory Syndrome Virus Co-infection With Other Pathogens in Swine

et al. 2021

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The porcine reproductive and respiratory syndrome virus (PRRSV) is the pathogen causing epidemics of porcine reproductive and respiratory syndrome (PRRS), and is present in every major swine-farming country in the world. Previous studies have demonstrated that PRRSV infection leads to a range of consequences, such as persistent infection, secondary infection, and co-infection, and is common among pigs in the field. In recent years, coinfection of PRRSV and other porcine pathogens has occurred often, making it more difficult to define and diagnose PRRSV-related diseases. The study of coinfections may be extremely suitable for the current prevention and control in the field. However, there is a limited understanding of coinfection. Therefore, in this review, we have focused on the epidemiology of PRRSV coinfection with other pathogens in swine, both in vivo and in vitro.

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“…The nsp2-encoding region is highly diverse and variable in the PRRSV genome, where substitutions, deletions and insertions usually occur naturally [45] . In particular, gene deletions in the nsp2 hypervariable region often appeared in the field isolates or in vitro cultures of PRRSV, such as the representative PRRSV isolates NADC30-like, Em2007 and GDHY from China, and MN184 from the United States [46][47][48][49][50] . These isolates contain an over 30-amino acid (aa)-deletion in the nsp2 region relative to the North American prototype strain VR-2332 [46][47][48][49][50] .…”

Section: Insertion Of Foreign Genes Into the Nonessential Region In Nsp2mentioning

confidence: 99%

“…PRRSV belongs to the genus Porarterivirus, together with the other four genera, Equarterivirus, Simarterivirus, Diparterivirus, Nesarterivirus, placing within the family Arteriviridae of the order Nidovirales [49] . This family includes other members, such as lactate dehydrogenase-elevating virus of mice (LDV), simian hemorrhagic fever virus (SHFV), equine arterivirus (EAV) and the recently described wobbly possum disease virus (WPDV) [1,4,5] .…”

Section: Introductionmentioning

confidence: 99%

Research progress in the development of porcine reproductive and respiratory syndrome virus as a viral vector for foreign gene expression and delivery

Dai

Huang

Fung

et al. 2020

Expert Review of Vaccines

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Epidemiological and genetic characteristics of porcine reproductive and respiratory syndrome virus circulating in central and South China in 2016

Cited by 17 publications

References 31 publications

Molecular Characterization of the Nsp2 and ORF5 (ORF5a) Genes of PRRSV Strains in Nine Provinces of China During 2016–2018

Molecular Characterization of the Nsp2 and ORF5 (ORF5a) Genes of PRRSV Strains in Nine Provinces of China During 2016–2018

Advanced Research in Porcine Reproductive and Respiratory Syndrome Virus Co-infection With Other Pathogens in Swine

Research progress in the development of porcine reproductive and respiratory syndrome virus as a viral vector for foreign gene expression and delivery

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