Epidemiological and molecular profile of blood donors infected with HTLV-1/2 in the state of Pará, northern Brazil

Maneschy, Carolina de Alcântara; Barile, Katarine Antônia dos Santos; Castro, Jairo Augusto Américo de; Palmeira, Maurício Koury; Castro, Renata Bezerra Hermes de; Amaral, Carlos Eduardo de Melo

doi:10.1007/s42770-021-00609-w

Cited by 6 publications

(6 citation statements)

References 28 publications

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“…However, HTLV infection is not included on the list of notifiable diseases in most Brazilian states, thus the available epidemiological data are usually from specific population groups such as blood donors, pregnant women, injecting drug users and sex workers 9,10 . Regarding the epidemiology among blood donors in the State of Pará, some recent HTLV seroprevalence studies, previously conducted by our group, described prevalence rates of 0.2% 8 and 0.3% 11 , but only 22% of such reactive samples confirmed the presence of HTLV-1/2 proviral DNA 11 .…”

Section: Introductionmentioning

confidence: 74%

Results of a sample-to-cutoff ratio using Abbott Architect rHTLV-I/II assay allow to predict detection of HTLV-1 and HTLV-2 proviral DNA by real-time PCR

Maneschy

Barile

Palmeira

et al. 2022

Rev Inst Adolfo Lutz

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The present study aims to correlate the sample-to-cutoff ratios (S/CO) distributions of reactive results for HTLV-1/2 antibodies with the detection of proviral DNA in a population of blood donor candidates. It was carried out a retrospective data search of 632 HTLV-1/2 reactive samples, submitted to confirmatory testing from January 2015 to December 2019. Serological screening was performed by chemiluminescent microparticle immunoassay Architect rHTLV-I/II, whereas confirmatory testing was performed by in-house real-time polymerase chain reaction method. 496 out of 632 samples (78%) had undetectable HTLV-1/2 proviral DNA and 136 (22%) had detectable proviral DNA. HTLV infection was not confirmed in any individual for whom the S/CO ratio value was <4, and proviral DNA detection rates gradually escalated as S/CO ratio values increased. The sensitivity and predictive positive value found for the Architect rHTLV-I/II was 100% and 22%, respectively. The receiver operating characteristic (ROC) curve analysis showed that the optimal S/CO ratio value for predicting the presence of HTLV-1/2 was 18.11. High S/CO ratios were more associated with the detection of proviral DNA. The S/CO ratio value <4 suggests excluding true HTLV infection and the risk of blood transmission.

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Section: Introductionmentioning

confidence: 74%

Results of a sample-to-cutoff ratio using Abbott Architect rHTLV-I/II assay allow to predict detection of HTLV-1 and HTLV-2 proviral DNA by real-time PCR

Maneschy

Barile

Palmeira

et al. 2022

Rev Inst Adolfo Lutz

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“…A sua subfamília é Oncovirinae, existindo quatro sorotipos conhecidas. Os mais relevantes são HTLV-I, mencionado pela primeira vez na década de 1980, é o primevo de retrovírus ontogênico humano, correlacionado com o risco de desenvolver polimiosite, TSP, ATLL, uveíte, dermatite infecciosa e mieloplasia associada à HTLV (HAM) (De Oliveira, Kachimarek, Bittencourt, 2018;Santos et al, 2020;Maneschy, 2021).No caso do HTLV-II, apenas isolou-se em 1982, tornando-se o segundo retrovírus linfotrópico, retirou-se a amostra de tribos indígenas e de usuários de drogas intravenosas na parte ocidental, pesquisas apontam que podem esta relacionados a síndromes neurológicas similares a HAM, TSP, além da elevada incidência de infecções respiratórias (bronquite, pneumonia, tuberculose), do trato urinário e dermatológico (abscesso de pelos e tecidos) (Santos et al, 2020;Siravelha, 2018;Garcia, 2019).…”

Section: Resultsunclassified

“…Especula-se que cerca de 10 a 20 milhões de indivíduos contaminados pelo HTLV no mundo. O Brasil atinge as primeiras posições do ranking, relacionado diretamente pela extensão da população, em torno de 2,5 milhões de pessoas eivadas pelo vírus (Maneschy, 2021;Paiva, 2017;Siravelha, 2018). Há indícios que a introdução do vírus ao país efetuou-se pela chegada dos feudatários provenientes da África, alegando a elevada taxa de infecções em afrodescendentes.…”

Section: Introductionunclassified

Soroprevalência de HTLV em gestantes: revisão integrativa

Mendes¹,

Pacheco²,

Silva³

et al. 2021

RSD

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Introdução: Especula-se que cerca de 10 a 20 milhões de indivíduos contaminados pelo HTLV no mundo. O Brasil atinge as primeiras posições do ranking. Os meios de propagação do vírus ocorrem por aleitamento materno, por transmissão vertical, sexual e parenteral. O marcador para identificar o alto risco de TMI, são as PVL de HLTV-1 em células mononucleares no sangue periférico da gestante. Objetivo: averiguar a soroprevalência de HTLV nas gestantes, efetivando uma revisão integrativa do assunto em questão e analisar as formas preventivas para a progênie do hospedeiro. Metodologia: Refere-se a uma revisão integrativa utilizando bancos de dados online como: Google School, BIREME, SCIELO, PUBMED, FIOCRUZ, MS, USP, LILACS, Science Direct. Resultado e Discussões: Com a complexidade de diagnósticos de casos leves, localizadas no plasma e a escassez de testes confirmatórios deste, pela detecção do anti-HTLV por PCR, ELISA ou Western Blot, acarreta complicações para diagnostico de ATLL em adultos de 20 a 30 anos, que ocorre posteriormente à infecção na criança. O aleitamento materno torna-se a principal via de transmissão, superando os casos relatados por via sexual e parenteral. Contudo, como a investigação sobre transmissão, não é empregue no Brasil, seja por via sexual ou TMI, ocasiona endemia recorrente no país. Tornando a prevenção da incidência o meio mais adequado ao combate logo não contêm vacinas ou tratamento. Conclusão: O SUS carece no aprimoramento das pesquisas epidemiológicas para averiguar a endemicidade e proporcionar o rastreamento e auxilio as gestantes soropositivas.

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“…At the time, the child had 2 years old and did not present a seroconversion. The need for an improvement of maternal counseling about breastfeeding was already reported in the state 11 , demonstrating that infection patterns are not so well understood by health workers 22 when they are expected that they should be able to properly provide counseling for positive patients about HTLV care and prevention.…”

Section: Discussionmentioning

confidence: 99%

“…Regarding inconsistencies found in the answers given to HEMOSUL and the research group, it may be that some blood donors see the blood banks as a convenient place for free testing 22 . The default in marital status found in this study may be due to the omission of risk factors, such as possibly multiple partners.…”

Section: Discussionmentioning

confidence: 99%

HTLV infection in blood donors from Mato Grosso do Sul state: a closer look at HTLV screening in Brazilian blood banks

Amianti,

Bandeira,

Romeiro

et al. 2023

Sci Rep

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Human T-lymphotropic virus (HTLV) infection has a worldwide distribution and currently, more than 2.5 million individuals have been infected in Brazil. The study aimed to investigate HTLV infection prevalence among blood donors in Mato Grosso do Sul, characterizing seroepidemiological profiles of HTLV-1/2 positive individuals and evaluating the blood bank's HTLV screening system. A cross-sectional survey was conducted among blood donors from Mato Grosso do Sul state (MS)—Central Brazil, between January to December 2021. The information was obtained from databases, samples from the collection of HEMOSUL, and active searching, with the completion of laboratory analyses. 35,278 blood donors were screened for anti-HTLV-1/2 by chemiluminescence immunoassay (CMIA). Among them, 78 were initially reactive for anti-HTLV-1/2 (2.21/1000). Out of 78, 67 returned to the blood center to collect a second sample for retesting with a second screening with CMIA. After confirmation, 8 samples were indeterminate, and 8 were confirmed as positive for HTLV antibodies. New tests were performed for the 8 positive samples, and 6 were confirmed as HTLV-1 infection (0.17/1,000), one as negative, and one as indeterminate. The present study describes the low prevalence of HTLV infection in blood donors from MS and contributes to the definition of the regional infection profile. The prevalence found in this study (0.017%–0.17/1000) shows to be a much lower value than the rates reported in other states in Brazil. We highlight the need for confirmatory testing for those seropositive donors in screening assays and the need for adequate counseling and patient management for those confirmed HTLV individuals.

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Epidemiological and molecular profile of blood donors infected with HTLV-1/2 in the state of Pará, northern Brazil

Cited by 6 publications

References 28 publications

Results of a sample-to-cutoff ratio using Abbott Architect rHTLV-I/II assay allow to predict detection of HTLV-1 and HTLV-2 proviral DNA by real-time PCR

Results of a sample-to-cutoff ratio using Abbott Architect rHTLV-I/II assay allow to predict detection of HTLV-1 and HTLV-2 proviral DNA by real-time PCR

Soroprevalência de HTLV em gestantes: revisão integrativa

HTLV infection in blood donors from Mato Grosso do Sul state: a closer look at HTLV screening in Brazilian blood banks

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