Epidemiological Clues for Developing Methods of Control of Bacterial Blight of Cantaloupe Caused by Pseudomonas syringae pv. aptata

Riffaud, Christine; Glaux, Catherine; Guilbaud, Caroline; Prior, Philippe; Morris, Casey; Domínguez, Helena

doi:10.1007/978-94-017-0133-4_1

Cited by 13 publications

(9 citation statements)

References 14 publications

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“…Bacterial leaf spot of watermelon ( Citrullus lanatus ), cantaloupe ( Cucumis melo ), and squash ( Cucurbita pepo ) is a common early spring disease that has a worldwide distribution and can cause significant economic losses under cool, wet environmental conditions (Morris et al, 2000; Riffaud et al, 2003; Newberry et al, 2017). The disease was recently recognized as a seedborne disorder of squash (Manceau et al, 2011); however, its etiology in various cucurbit species is likely to have multiple sources (Monteil et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash

et al. 2019

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Pseudomonas syringae sensu stricto (phylogroup 2; referred to as P. syringae ) consists of an environmentally ubiquitous bacterial population associated with diseases of numerous plant species. Recent studies using multilocus sequence analysis have indicated the clonal expansion of several P. syringae lineages, located in phylogroups 2a and 2b, in association with outbreaks of bacterial spot disease of watermelon, cantaloupe, and squash in the United States. To investigate the evolutionary processes that led to the emergence of these epidemic lineages, we sequenced the genomes of six P. syringae strains that were isolated from cucurbits grown in the United States, Europe, and China over a period of more than a decade, as well as eight strains that were isolated from watermelon and squash grown in six different Florida counties during the 2013 and 2014 seasons. These data were subjected to comparative analyses along with 42 previously sequenced genomes of P. syringae stains collected from diverse plant species and environments available from GenBank. Maximum likelihood reconstruction of the P. syringae core genome revealed the presence of a hybrid phylogenetic group, comprised of cucurbit strains collected in Florida, Italy, Serbia, and France, which emerged through genome-wide homologous recombination between phylogroups 2a and 2b. Functional analysis of the recombinant core genome showed that pathways involved in the ATP-dependent transport and metabolism of amino acids, bacterial motility, and secretion systems were enriched for recombination. A survey of described virulence factors indicated the convergent acquisition of several accessory type 3 secreted effectors (T3SEs) among phylogenetically distinct lineages through integrative and conjugative element and plasmid loci. Finally, pathogenicity assays on watermelon and squash showed qualitative differences in virulence between strains of the same clonal lineage, which correlated with T3SEs acquired through various mechanisms of horizontal gene transfer (HGT). This study provides novel insights into the interplay of homologous recombination and HGT toward pathogen emergence and highlights the dynamic nature of P. syringae sensu lato genomes.

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Section: Introductionmentioning

confidence: 99%

Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash

et al. 2019

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“…lachrymans , WH 6/01 and WM 2/02, also exhibited pectolytic activity: P. syringae pv. lachrymans has been shown to be one of the few P. syringae pathovars that frequently possess pectolytic activity (Riffaud et al ., 2003). Both PCR‐RFLP and ADSRRS revealed significant diversity among P. syringae pv.…”

Section: Discussionmentioning

confidence: 99%

“…This heterogeneity was revealed by the molecular tests; dendrograms grouped P. viridiflava ‐like strains into different clusters. Previously, P. viridiflava has been identified on Cucumis species (Goumans & Chatzaki, 1998; Riffaud et al ., 2003; Moretti et al ., 2005), and isolated from cucumber or melon leaves with identification based on biochemical and physiological tests. In this study, the LOPAT results for cucumber strains WK 2/02, WK 8/02, WK 9/02, WK 10/02, WW 5/02 and WW 6/02 indicated that these strains were P. viridiflava ‐like (Table 1).…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, although pectolytic activity is typical for P. viridiflava strains, P. syringae pv. lachrymans have also been reported to exhibit this property (Riffaud et al ., 2003). The former has been shown to cause necrotic lesions on plant leaves without water‐soaked symptoms and plants readily recover from the infection (Moretti et al ., 2005) and similar results were observed in this study for strains classified as P. viridiflava ‐like.…”

Section: Discussionmentioning

confidence: 99%

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Genetic diversity of Pseudomonas syringae pv. lachrymans strains isolated from cucumber leaves collected in Poland

et al. 2006

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Several strains of Pseudomonas syringae pathovar (pv.) lachrymans and related bacterial pathogens were isolated from cucumber ( Cucumis sativus ) leaves collected in central and southern Poland in 2001 and 2002. Twenty five original strains, together with five reference strains of P. syringae pv. lachrymans , pv. syringae and pv. tomato , were genetically characterized by PCR-RFLP (polymerase chain reaction − restriction fragment length polymorphism), ADSRRS (amplification of DNA fragments surrounding rare restriction sites), and PCR-MP (PCR − melting profiles) fingerprinting techniques. Genetic similarity analyses of the PCR-RFLP and ADSRRS fingerprints showed that strains of P. syringae pv. lachrymans form distinct clusters. The results also indicated that the ADSRRS and the PCR-MP fingerprinting techniques may serve as more efficient tools for evaluating genetic similarity among pathovars and strains of P. syringae than PCR-RFLP. The 25 strains showed diverse pathogenicity to cucumber seedlings and biochemical tests were varied. The syrB gene was identified in four cucumber strains, characterized as P. syringae pv. syringae .

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“…Significant variability in virulence, aggressiveness and genome structure has been reported among the numerous strains of P. syringae pv. lachrymans that have been identified (Riffaud et al ., 2003; Stead et al ., 2003; Olczak‐Woltman et al ., 2007). This variability necessitates caution in selecting a suitable strain for use in evaluating cucumber germplasm and in the analysis of the mode of inheritance of resistance to ALS.…”

Section: Introductionmentioning

confidence: 99%

Inheritance of resistance to angular leaf spot (Pseudomonas syringae pv. lachrymans) in cucumber and identification of molecular markers linked to resistance

et al. 2009

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Angular leaf spot is a common disease of cucumber ( Cucumis sativus ) caused by Pseudomonas syringae pv. lachrymans . Genetics of resistance to this disease was investigated using two sets of parameters: (i) disease severity, i.e. the number and size of necrotic and chlorotic lesions on the infected leaves, and (ii) presence or absence of a chlorotic halo around the necrotic spots on the infected leaves. Disease severity appears to be controlled by multiple genes and the heritability of the resistance was estimated to be 53%. The presence or absence of the chlorotic halo was determined to be governed by a single gene, with the presence of the halo (the susceptible phenotype) being a dominant character. A RAPD marker linked to the gene conferring the chlorotic halo was identified. Genetic distance between this marker, OP-AO07, a polymorphic 420 bp amplicon in the DNA of the susceptible plants, and the locus encoding the chlorotic halo was estimated to be 13 cM.

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Epidemiological Clues for Developing Methods of Control of Bacterial Blight of Cantaloupe Caused by Pseudomonas syringae pv. aptata

Cited by 13 publications

References 14 publications

Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash

Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash

Genetic diversity of Pseudomonas syringae pv. lachrymans strains isolated from cucumber leaves collected in Poland

Inheritance of resistance to angular leaf spot (Pseudomonas syringae pv. lachrymans) in cucumber and identification of molecular markers linked to resistance

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