Epidemiological Data on Pathogenic<i>Yersinia enterocolitica</i>in Southern Germany During 2000–2006

Bucher, Michael; Meyer, Cornelia; Grötzbach, Britta; Wacheck, Silke; Stolle, A.; Fredriksson-Ahomaa, Maria

doi:10.1089/fpd.2007.0076

Cited by 92 publications

(76 citation statements)

References 32 publications

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“…On the other hand, ELISA assays using specific antigens against Y. enterocolitica serotype O:3 (Nielsen et al, 1996) may detect antibodies against the most common pathogenic serotype in pigs, but not the more rare Y. enterocolitica serotypes and Y. pseudotuberculosis. Nevertheless, as in most European countries, Y. enterocolitica serotype O:3 predominates in pigs (Bucher et al, 2008;Ortiz Martínez et al, 2009;Van Damme et al, 2010), this may have a small impact on the final outcome.…”

Section: Discussionmentioning

confidence: 94%

“…enterocolitica is mainly transmitted to humans through the consumption of contaminated pork (Fosse et al, 2008;Rosner et al, 2012). A high proportion of pigs at slaughter are infected with this pathogen, mainly bioserotype 4/O:3 (Bucher et al, 2008;Ortiz Martínez et al, 2009). Y. pseudotuberculosis is also found in slaughter pigs, though to a lesser extent than Y. enterocolitica (Ortiz Martínez et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Relation Between Serology of Meat Juice and Bacteriology of Tonsils and Feces for the Detection of EnteropathogenicYersiniaspp. in Pigs at Slaughter

Damme

Vanantwerpen

Berkvens

et al. 2014

Foodborne Pathogens and Disease

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The association between positive serology and culture detection of Yersinia spp. in individual pigs was determined. Pieces of diaphragm from 370 pig carcasses were collected for serological analysis, and tonsils and feces of the same carcass were collected for bacteriological analysis. Detection of anti-Yersinia antibodies in meat juice samples was done using an indirect enzyme-linked immunosorbent assay (ELISA) based on Yops (Yersinia outer proteins). Tonsils and feces were tested for the presence of enteropathogenic Yersinia spp. by direct plating on cefsulodin-irgasan-novobiocin agar plates. Of the 370 meat juice samples, 241 (65.1%) gave a positive serological reaction using a cutoff value of 20%. Enteropathogenic Yersinia spp. (Yersinia enterocolitica serotype O:3 and Yersinia pseudotuberculosis) were found in tonsils of 161 pigs and feces of 30 pigs. Recovery of enteropathogenic Yersinia from the tonsils was highly correlated with positive serotiters, whereas no correlation was found between serology and fecal excretion. Results demonstrated that serology has an acceptable sensitivity, but a relatively low specificity for the rapid detection of enteropathogenic Yersinia spp. in tonsils of pigs at slaughter.

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Relation Between Serology of Meat Juice and Bacteriology of Tonsils and Feces for the Detection of EnteropathogenicYersiniaspp. in Pigs at Slaughter

Damme

Vanantwerpen

Berkvens

et al. 2014

Foodborne Pathogens and Disease

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“…In 2014, 6839 confirmed cases of yersiniosis were reported in Europe (18 cases in Italy), with an overall rate of 1.8 cases per 100,000 population (15). Infection can arise from ingestion of contaminated foods, in particular pork and pork products (6,10,26,30), or infected water (16,30). Moreover, in several cases of clinical sepsis, Y. enterocolitica infection was documented to be caused by using contaminated products in blood transfusions (18,20).…”

Section: Introductionmentioning

confidence: 99%

A rare case of enteric and systemic Yersinia enterocolitica infection in a chronic, not iron-overloaded dialysis patient

et al. 2017

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SummaryWe present herein a case of bacterial gastroenteritis due to Yersinia enterocolitica, occurred in a young woman undergoing haemodialysis with a previous history positive for prolonged (20 years) immunosuppressive therapy for glomerulonephritis before and for kidney transplant later. The patient's outcome was favourable after a third-generation cephalosporin treatment without complications. The possible pathophysiological association between patient clinical condition and Yersinia bacteraemia is discussed, along with the review of literature.

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“…Yersinia enterocolitica belongs to Gram-negative bacteria which constitute a very heterogeneous group and include different bioserotypes, among which only yersiniosis and an important foodborne pathogen responsible for provoking gastrointestinal diseases in humans (Bucher et al 2008, Galindo et al 2011. The most common transmission routes of bacteria include the route directly from pigs, via the consumption of contaminated pork products or indirectly from pets that were fed raw pork (EFSA 2011, Laukkanen--Ninios et al 2014, EFSA and ECDC 2015, Van Damme et al 2015.…”

Section: Introductionmentioning

confidence: 99%

Detection of Yersinia Enterocolitica Species in Pig Tonsils and Raw Pork Meat by the Real-Time Pcr and Culture Methods

Ma¹

2017

Polish Journal of Veterinary Sciences

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The aim of the present study was to establish a rapid and accurate real-time PCR method to detect pathogenic Yersinia enterocolitica in pork. Yersinia enterocolitica is considered to be a crucial zoonosis, which can provoke diseases both in humans and animals. The classical culture methods designated to detect Y. enterocolitica species in food matrices are often very time-consuming. The chromosomal locus -tag CH49 -3099 gene, that appears in pathogenic Y. enterocolitica strains, was applied as DNA target for the 5' nuclease PCR protocol. The probe was labelled at the 5' end with the fluorescent reporter dye (FAM) and at the 3' end with the quencher dye (TAMRA). The real-time PCR cycling parameters included 41 cycles. A Ct value which reached a value higher than 40 constituted a negative result.The developed for the needs of this study qualitative real-time PCR method appeared to give very specific and reliable results. The detection rate of locus -tag CH49 -3099 -positive Y. enterocolitica in 150 pig tonsils was 85 % and 32 % with PCR and culture methods, respectively. Both the Real-time PCR results and culture method results were obtained from material that was enriched during overnight incubation. The subject of the study were also raw pork meat samples. Among 80 samples examined, 7 ones were positive when real-time PCR was applied, and 6 ones were positive when classical culture method was applied. The application of molecular techniques based on the analysis of DNA sequences such as the Real-time PCR enables to detect this pathogenic bacteria very rapidly and with higher specificity, sensitivity and reliability in comparison to classical culture methods.

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Epidemiological Data on PathogenicYersinia enterocoliticain Southern Germany During 2000–2006

Cited by 92 publications

References 32 publications

Relation Between Serology of Meat Juice and Bacteriology of Tonsils and Feces for the Detection of EnteropathogenicYersiniaspp. in Pigs at Slaughter

Relation Between Serology of Meat Juice and Bacteriology of Tonsils and Feces for the Detection of EnteropathogenicYersiniaspp. in Pigs at Slaughter

A rare case of enteric and systemic Yersinia enterocolitica infection in a chronic, not iron-overloaded dialysis patient

Detection of Yersinia Enterocolitica Species in Pig Tonsils and Raw Pork Meat by the Real-Time Pcr and Culture Methods

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