Epidemiological distribution of hepatitis B virus genotypes in 1–29-year-olds in the mainland of China

Su, Qiudong; Zhang, Shuang; Wang, Rui; Li, He; Zhang, Guomin; Zheng, Hui; Qiu, Feng; Sun, Xiaojin; Liang, Xiaofeng; Bi, Shengli; Shen, Liping; Wang, Fu-zhen

doi:10.1016/j.vaccine.2020.09.083

Cited by 13 publications

(13 citation statements)

References 27 publications

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“…Several investigations have indicated that different HBV genotypes could affect the clinical outcomes and response to antiviral treatment in hepatitis B patients. Genotypes B and C are the two most common agents (accounting for approximately 95%) in China (Lin and Kao, 2011;Wang et al, 2019;Su et al, 2020). Previous studies demonstrated that HBV genotype C was associated with a higher risk of reactivation of hepatitis B, with more severe liver fibrosis, and can more easily progress to hepatocellular carcinoma than genotype B infection (Lin and Kao, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…In clinical practice, the genotype characteristic is essential for finding the severity of HBV infection and response to antiviral therapy in hepatitis B patients (Wang et al, 2015;Zhao et al, 2010). China has a high incidence of HBV, and genotypes B and C were identified as the most common agents (more than 95%) (Lin and Kao, 2011;Wang et al, 2019;Su et al, 2020). HBV genotype C causes more severe liver fibrosis, which more easily progresses to hepatocellular carcinoma, than genotype B infection (Zhao et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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A CRISPR-Cas12b–Based Platform for Ultrasensitive, Rapid, and Highly Specific Detection of Hepatitis B Virus Genotypes B and C in Clinical Application

Tan²,

Wang

et al. 2021

Front. Bioeng. Biotechnol.

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Hepatitis B virus (HBV) is one of the most dangerous and prevalent agents that causes acute and chronic liver diseases in humans. Genotyping plays an important role in determining clinical outcomes and response to antiviral treatment in HBV–infected patients. Here, we first devised a CRISPR–based testing platform, termed “CRISPR-HBV,” for ultrasensitive, highly specific, and rapid detection of two major HBV genotypes (HBV-B and HBV-C) in clinical application. The CRISPR-HBV employed multiple cross displacement amplification (MCDA) for rapid preamplification and then Cas12b–based detection for decoding the targets. Finally, the detection result was read out with real-time fluorescence and a lateral flow biosensor. The sensitivity of CRISPR-HBV was 10 copies per test. The specificity was one hundred percent, and no cross reactions were observed in other HBV genotypes and pathogens. The whole detection process, including DNA template extraction (15 min), preamplification reaction of MCDA (30 min at 65°C), CRISPR-Cas12b–based detection (5 min at 37°C), and results readout (∼2 min), could be completed within 1 h. The feasibility of the CRISPR-HBV assay for genotyping HBV-B and -C as successfully validated with clinical samples. Hence, the CRISPR-HBV assay has remarkable potential to develop a point-of-care testing for identifying and distinguishing HBV genotypes B and C in clinical settings, especially in resource-scarcity countries.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A CRISPR-Cas12b–Based Platform for Ultrasensitive, Rapid, and Highly Specific Detection of Hepatitis B Virus Genotypes B and C in Clinical Application

Tan²,

Wang

et al. 2021

Front. Bioeng. Biotechnol.

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“…As expected, TGF-β1 has a strong association with HBV replication and HBV related liver diseases (10,11). TGF-β1 has been demonstrated to serve important roles in HBV induces liver fibrosis via the TGF-β1/miR-21-5p pathway WENTING LI 1,2* , XIAOLAN YU 2,3* , XILIU CHEN 4* , ZHENG WANG 5 , MING YIN 2,6 , ZONGHAO ZHAO 1,2 and CHUANWU ZHU 7 hepatic stellate cell (HSC) activation, which is a key event in liver fibrosis (11,12). Accumulating evidence suggested that HBV infection may promote TGF-β1 production by hepatocytes, which in turn activates HSCs and accelerates liver fibrosis (13)(14)(15).…”

Section: Introductionmentioning

confidence: 58%

“…Liver fibrosis has long been considered as a healing response to various chronic liver injuries, including viral hepatitis, immune compounds and toxic agents, among others (1). Hepatitis B virus (HBV) remains the most important cause of liver fibrosis in China, which is an endemic area (2). During the life cycle, HBV generates covalently closed circular DNA (cccDNA), which acts as the model of HBV replication (3).…”

Section: Introductionmentioning

confidence: 99%

HBV induces liver fibrosis via the TGF‑β1/miR‑21‑5p pathway

Chen

et al. 2020

Exp Ther Med

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MicroRNA (miR)-21-5p is a newly discovered factor that mediates TGF-β1 signaling. The present study was designed to investigate the role of TGF-β1/miR-21-5p in hepatitis B virus (HBV)-induced liver fibrosis. HBV-infected sodium taurocholate co -t ra nspor ting polypeptide (NTCP)-transfected Huh7.5.1 cells were co-cultured with LX2 cells to simulate HBV infection in the present study. A total of 29 patients with chronic HBV infection were enrolled. Cells were transfected with miR-21-5p mimic or inhibitor with or without TGF-β1 stimulation. The demographic, biochemical and virological data from the 29 patients were analyzed and liver tissues were collected. miR-21-5p levels and the mRNA and protein expression of α-smooth muscle actin (SMA), collagen type 1 α 1 (CoL1A1), tissue inhibitor of metalloproteinase (TIMP)-1 and Smad from liver cells or tissues were detected by quantitative PCR analysis and western blotting, respectively. Cell viability was observed, and the liver fibrosis score was evaluated. The association between miR-21-5p and liver fibrosis was evaluated by correlation analysis. HBV infection upregulated TGF-β1/miR-21-5p mRNA expression in NTCP-Huh7.5.1 cells compared with mock infection (P<0.05). TGF-β1 incubation significantly increased miR-21-5p levels, as well as the mRNA and protein expression of α-SMA, CoL1A1 and TIMP-1, and reduced Smad7 expression in LX2 cells compared with the normal group, and these effects were counteracted by miR-21-5p inhibitor (P<0.05). miR-21-5p overexpression also contributed to TGF-β1-induced α-SMA, CoL1A1 and TIMP-1 expression in LX2 cells (P<0.05). Co-culture with HBV-infected NTCP-Huh7.5.1 cells upregulated TGF-β1/miR-21-5p activity and CoL1A1 expression in LX2 cells compared with normal control, which were significantly reduced by miR-21-5p inhibitor (P<0.05). miR-21-5p levels were significantly correlated with the liver fibrosis score (r=0.888; P<0.05). These data demonstrated that HBV induced liver fibrosis via the TGF-β1/miR-21-5p pathway and suggested that miR-21-5p may be an effective anti-fibrosis target.

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“…So it may be that abnormal liver function due to hepatitis virus infection causes a statistical correlation between TN occurrence and liver function, and hepatitis B virus infection is a serious public health problem in China, where tens of millions of hepatitis B virus carriers. 34 , 35 The association between hepatitis B virus infection and TN requires our attention, and further studies are necessary to investigate the underlying mechanisms causing this phenomenon.…”

Section: Discussionmentioning

confidence: 99%

Correlation Analysis of Breast and Thyroid Nodules: A Cross-Sectional Study

Chen¹,

Hou²,

Tang³

et al. 2021

IJGM

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Based on physical examination, to explore the relationship between breast mass (BM) and thyroid nodule (TN) prevalence, and to further explore other related factors that affect the occurrence of BM and TN. Methods: From January 1, 2018, to January 1, 2021, 12,538 female subjects received breast and thyroid ultrasound examinations at the same time in the health examination center of the Affiliated Hospital of North Sichuan Medical College. Univariate analysis and multivariate logistic analysis were used to screen the relevant factors affecting TN and BM, and propensity score matching was used to further verify the results of the relationship between breast and thyroid. Results: A total of 4975 (39.7%) of the included subjects have BM and a total of 6315 (50.4%) have TN,2557 (20.4%) had both BM and TN. The logistic regression results show that patients with TN are more likely to suffer from BM [OR = 1.185, 95% CI (1.099-1.278), p<0.0001]. In addition, age, free T4, HDL, height, BMI, systolic blood pressure, diastolic blood pressure, and albumin are independent factors affecting the occurrence of BM; patients with BM are more likely to have TN [OR = 1.180, 95% CI (1.094-1.272), p<0.0001], and age, free T3, free T4, AST, ALT, albumin, height, and BMI are independent influencing factors on the occurrence of TN. The result of propensity score matching confirmed the relationship between BM and TN. Conclusion:There is a bidirectional pathogenic relationship between BM and TN, women with BM are at increased risk of TN, and women with TN are more likely to have BM. Thyroid hormone is not only related to the occurrence of TN but also affects the occurrence of BM.

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Epidemiological distribution of hepatitis B virus genotypes in 1–29-year-olds in the mainland of China

Cited by 13 publications

References 27 publications

A CRISPR-Cas12b–Based Platform for Ultrasensitive, Rapid, and Highly Specific Detection of Hepatitis B Virus Genotypes B and C in Clinical Application

A CRISPR-Cas12b–Based Platform for Ultrasensitive, Rapid, and Highly Specific Detection of Hepatitis B Virus Genotypes B and C in Clinical Application

HBV induces liver fibrosis via the TGF‑β1/miR‑21‑5p pathway

Correlation Analysis of Breast and Thyroid Nodules: A Cross-Sectional Study

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