Epidemiological features of viral encephalitis in Cangzhou of China with use of multiplex RT-PCR for five RNA viruses

Kong, Xiangjun; Zhang, Lei; Liu, Kehun; Hu, Chen; Li, Baohui; Wu, Rui; Ji, Chunxue

doi:10.1016/j.jviromet.2015.06.009

Cited by 9 publications

(5 citation statements)

References 9 publications

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“…The etiology of VE shows geographical variation . EVs have been reported as the leading causes of VE in China . HSV was the most common causative agent of VE in the United States, England, France, Spain and eastern India, while JEV was reported as the primary viral pathogen responsible for VE among children in southern Vietnam and Thailand .…”

Section: Discussionmentioning

confidence: 99%

Serotypes of human enteroviruses causing pediatric viral encephalitis and meningitis in Hebei province, China, from 2013 to 2015

Chen

Guo

et al. 2018

Pediatric Investigation

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Importance: Viral encephalitis and meningitis are severe infectious diseases responsible for substantial morbidity and mortality in children. Enteroviruses are typically the most common causative agents of viral encephalitis and meningitis. Objective: This study aimed to investigate the etiology of viral encephalitis and meningitis among children in Hebei province, China. Methods: Cerebrospinal fluid samples from children with viral encephalitis (n=309) and meningitis (n=133) were collected between Nov 2013 and Dec 2015 and viral pathogens were identified by real-time and multiplex PCR. Amplification and sequencing of partial VP1 genes was used to type enteroviruses. Results: The causative pathogen was successfully detected in 176 (57%) patients with viral encephalitis and 82 (61.7%) patients with viral meningitis. The most common causative agents of both viral encephalitis and meningitis were enteroviruses (55.7% and 64.6% of cases, respectively). The most common enterovirus serotypes identified were echovirus 18, echovirus 6 and echovirus 30. Echovirus 18 accounted for 74.4% of all typed enteroviruses and caused a viral encephalitis and meningitis outbreak in Hebei province in 2015. By contrast, the major enterovirus serotypes circulating in 2014 were echovirus 6 and echovirus 30. Interpretation: Enteroviruses were the main causative agents of viral encephalitis and meningitis in children in Hebei province from Nov 2013 to Dec 2015. Echovirus 18 became the leading cause of viral encephalitis and meningitis for the first time in Hebei province in 2015.

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Section: Discussionmentioning

confidence: 99%

Serotypes of human enteroviruses causing pediatric viral encephalitis and meningitis in Hebei province, China, from 2013 to 2015

Chen

Guo

et al. 2018

Pediatric Investigation

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“…This finding suggests the potential mechanism between steroid hormone biosynthesis and VEVM pathogenesis. Low viral contents in CSF, difficulties in viral culture, and initial non-specific clinical features of VEVM can result in the misdiagnosis and the delayed treatment of VEVM (Kong et al, 2015;Gao et al, 2017). In our study, children with VEVM presented with many similar clinical characteristics when compared to those with other diseases, and the similarities also appeared between children with VEVMP and children with VEVMN (Supplementary data Table S1 and Figs.…”

Section: Discussionmentioning

confidence: 51%

“…For all VEVMC children, we applied polymerase chain reaction (PCR) or reverse transcription polymerase chain reaction (RT-PCR) to detect viruses in CSF, and the detailed experimental methods have been reported in our previous study (Zhu et al, 2021). The measured viruses, including enterovirus (EV), herpes simplex virus (HSV)-1, HSV-2, mumps virus (MuV), Epsteinbarr virus, cytomegalovirus, and varicella-zoster virus, which were the most prevalent pathogens among children with VEVM in China (Zhang et al, 2013;Kong et al, 2015;Xie et al, 2015;Ai et al, 2017). Among the 68 children with VEVMC, 43 children were positive in PCR or RT-PCR results (denoted as VEVMP), and 25 children were negative in PCR or RT-PCR results (denoted as VEVMN).…”

Section: Clinical and Etiological Diagnosis Of Vevmmentioning

confidence: 99%

“…Encephalitis and/or meningitis are central nervous system (CNS) diseases that involve inflammation of the brain parenchyma and/or meninges (Thompson et al, 2012). Viruses that cross the blood-brain barrier (BBB) cause the viral infections of CNS, which is the most prevalent cause of encephalitis and/or meningitis in pediatrics, called viral encephalitis and/or viral meningitis (VEVM) (Thompson et al, 2012;Kong et al, 2015;Ai et al, 2017). The clinical diagnosis of VEVM is established by clinical manifestation, laboratory detection, clinical examination, and neuroimaging (Mutton and Guiver, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Alterations of oral microbiota in Chinese children with viral encephalitis and/or viral meningitis

Liu

Zhu

et al. 2022

J Microbiol.

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The role of oral microbiota in viral encephalitis and/or viral meningitis (VEVM) remains unclear. In this hospital-based, frequency-matched study, children with clinically diagnosed VEVM (n = 68) and those with other diseases (controls, n = 68) were recruited. Their oral swab samples were collected and the oral microbiota was profiled using 16S rRNA gene sequencing. The oral microbiota of children with VEVM exhibited different beta diversity metrics (unweighted UniFrac distance: P < 0.001, R 2 = 0.025, Bray-curtis dissimilarity: P = 0.045, R 2 = 0.011, and Jaccard dissimilarity: P < 0.001, R 2 = 0.017) and higher relative abundances of taxa identified by Linear discriminant analysis (LDA) with effect size (Enterococcus, Pedobacter, Massilia, Prevotella_9, Psychrobacter, Butyricimonas, Bradyrhizobium, etc., LDA scores > 2.0) when compared with the control group. The higher pathway abundance of steroid hormone biosynthesis predicted by oral microbiota was suggested to be linked to VEVM (q = 0.020). Further, a model based on oral microbial traits showed good predictive performance for VEVM with an area under the receiver operating characteristic curve of 0.920 (95% confidence interval: 0.834-1.000). Similar results were also obtained between children with etiologically diagnosed VEVM (n = 43) and controls (n = 68). Our preliminary study identified VEVM-specific oral microbial traits among children, which can be effective in the diagnosis of VEVM.

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“…In Cangzhou city of China, the major pathogens of viral encephalitis were EVs with a positive rate of 27.8%. [5] And in Zhejiang province, the positive rate of viral encephalitis caused by EVs in cerebrospinal fluid (CSF) samples between 2002 and 2012 was 13.9%. [6] So in China, viral encephalitis caused by enterovriuses is worthy of attention because of high morbidity and mortality.…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiological study of enteroviruses associated with encephalitis in children from Hangzhou, China

2016

Medicine

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Enterovirus (EV) has over 100 serotypes of species A-D, which can cause various symptoms in infants. Enterovirus encephalitis (EVE) is serve disease with high morbidity and mortality in children. To well define the epidemiology of EVE, we wanted to know more about EV and EV molecular typing by conducting this study in Hangzhou.Cerebrospinal fluid samples were collected from children with diagnosis of encephalitis. Meanwhile, one-step real-time RT-PCR was used for the detection of EV, and we also identified the serotypes of EV by using gene sequencing of VP1 or 5 0 UTR region. A total of 126 CSF specimens were tested and EV was detected in 26 specimens (20.6%). The molecular typing results showed different types of EV strains including Coxsackievirus B2, Coxsackievirus B3, Echovirus 5, Echovirus 16, Echovirus 18, Echovirus 30, and all EV isolates belonging to the human EV species B.According to the sequence of VP1 and 5 0 UTR region, E30 may be major cause of children's EVE in Hangzhou, China.Abbreviations: CSF = cerebrospinal fluid, EV = enterovirus, EVE = enterovirus encephalitis, GLU = glucose, RT-PCR = reverse transcription-polymerase chain reaction.

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Epidemiological features of viral encephalitis in Cangzhou of China with use of multiplex RT-PCR for five RNA viruses

Abstract: The most common pathogens responsible for viral encephalitis in Cangzhou, Hebei province, China, were EVs, and the multiplex RT-PCR was a rapid, sensitive, accurate method of testing the viruses responsible for causing these illnesses.

Cited by 9 publications

References 9 publications

Serotypes of human enteroviruses causing pediatric viral encephalitis and meningitis in Hebei province, China, from 2013 to 2015

Serotypes of human enteroviruses causing pediatric viral encephalitis and meningitis in Hebei province, China, from 2013 to 2015

Alterations of oral microbiota in Chinese children with viral encephalitis and/or viral meningitis

Molecular epidemiological study of enteroviruses associated with encephalitis in children from Hangzhou, China

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