Epidemiological investigation and antimicrobial susceptibility analysis of ureaplasma species and<i>Mycoplasma</i>hominis in outpatients with genital manifestations

Song, Tiejun; Ye, Aiqing; Xie, Xinyou; Ruan, Zhi; Kong, Yingying; Song, Jinbao; Wang, Yue; Chen, Jiangzhong; Zhang, Jun

doi:10.1136/jclinpath-2014-202248

Cited by 42 publications

(30 citation statements)

References 16 publications

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“…Another Italian study, from De Francesco et al, obtained consistent results (10). Two Chinese analyses reported FQ resistance rates of Ͼ40% for Ureaplasma spp., while rates of resistance to macrolides and tetracyclines were Ͻ30% and Ͻ10%, respectively (8,9). In South Africa, Ureaplasma isolates had the following resistance rates: levofloxacin, 41%; moxifloxacin, 2%; ERY, 80%; TET, 73% (11).…”

Section: Resultsmentioning

confidence: 99%

“…Overall, in most countries the highest resistance rates have been recorded against FQs (8)(9)(10)(11)(12)(13)(14). This is undoubtedly due to their empirical overuse in the community (e.g., for urinary and respiratory tract infections), which contributes to the selection of amino acid substitutions in the QRDRs of DNA gyrase (GyrA and GyrB) and DNA topoisomerase IV (ParC and ParE) (20,26,27).…”

Section: Resultsmentioning

confidence: 99%

“…In general, these pathogens are considered susceptible to tetracyclines, fluoroquinolones (FQs), and macrolides (3). Acquired resistance against several of these classes has been described, but studies on the antimicrobial susceptibility profiles and resistance mechanisms of genital mycoplasmas are still scarce and cover only some geographic regions (8)(9)(10)(11)(12)(13)(14)(15).…”

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confidence: 99%

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Antibiotic Susceptibility and Sequence Type Distribution of Ureaplasma Species Isolated from Genital Samples in Switzerland

Schneider

Tinguely

Droz

et al. 2015

Antimicrob Agents Chemother

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Antibiotic resistance in Ureaplasma urealyticum/Ureaplasma parvum and Mycoplasma hominis is an issue of increasing importance. However, data regarding the susceptibility and, more importantly, the clonality of these organisms are limited. We analyzed 140 genital samples obtained in Bern, Switzerland, in 2014. Identification and antimicrobial susceptibility tests were performed by using the Mycoplasma IST 2 kit and sequencing of 16S rRNA genes. MICs for ciprofloxacin and azithromycin were obtained in broth microdilution assays. Clonality was analyzed with PCR-based subtyping and multilocus sequence typing (MLST), whereas quinolone resistance and macrolide resistance were studied by sequencing gyrA, gyrB, parC, and parE genes, as well as 23S rRNA genes and genes encoding L4/L22 ribosomal proteins. A total of 103 samples were confirmed as positive for U. urealyticum/U. parvum, whereas 21 were positive for both U. urealyticum/U. parvum and M. hominis. According to the IST 2 kit, the rates of nonsusceptibility were highest for ciprofloxacin (19.4%) and ofloxacin (9.7%), whereas low rates were observed for clarithromycin (4.9%), erythromycin (1.9%), and azithromycin (1%). However, inconsistent results between microdilution and IST 2 kit assays were recorded. Various sequence types (STs) observed previously in China (ST1, ST2, ST4, ST9, ST22, and ST47), as well as eight novel lineages, were detected. Only some quinolone-resistant isolates had amino acid substitutions in ParC (Ser83Leu in U. parvum of serovar 6) and ParE (Val417Thr in U. parvum of serovar 1 and the novel Thr417Val substitution in U. urealyticum). Isolates with mutations in 23S rRNA or substitutions in L4/L22 were not detected. This is the first study analyzing the susceptibility of U. urealyticum/U. parvum isolates in Switzerland and the clonality outside China. Resistance rates were low compared to those in other countries. We hypothesize that some hyperepidemic STs spread worldwide via sexual intercourse. Large combined microbiological and clinical studies should address this important issue.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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Antibiotic Susceptibility and Sequence Type Distribution of Ureaplasma Species Isolated from Genital Samples in Switzerland

Schneider

Tinguely

Droz

et al. 2015

Antimicrob Agents Chemother

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“…There are two species that exist in humans, Ureaplasma parvum (UPA) contains serovars 1, 3, 6, and 14 and Ureaplasma urealyticum (UUR) contains serovars 2, 4, 5, 7, 8, 9, 10, 11, 12, and 13 . About 15%‐30% of healthy adult men may harbor Ureaplasma spp in their lower urogenital tract . During the past decade, many investigators reported that Ureaplasma spp.…”

Section: Introductionmentioning

confidence: 99%

Detection of Ureaplasma spp. serovars in genital tract of infertile males

Song

Liu

Zhang

et al. 2019

Clinical Laboratory Analysis

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BackgroundThe colonization of Ureaplasma species in genital tract is related with male infertility. However, it has been postulated based upon limited study that virulence is related to serotype specificity. The aim of this study was to determine the distribution of Ureaplasma serovars in genital tract of infertile males and analyze their role in male infertility.MethodsA total of 358 urethral swabs samples were obtained from infertile males. The culture of Ureaplasma species were performed using a commercially available Mycoplasma IST 2 kit. Serovars were determined by real‐time polymerase chain reaction (real‐time PCR).ResultsA total of 92 (25.7%) infertile males were positive for Ureaplasma spp; among them, Ureaplasma parvum (UPA) was detected in 73 (79.3%) isolates, and Ureaplasma urealyticum (UUR) was detected in 19 (20.7%) isolates. Serovars 1, 6, or in combination accounted for 63.0% (46/73) of UPA isolates. Serovar 9 (alone and in combination of other serovars) was the most common serovar in UUR (47.4%, 9/19). Multiple serovars were detected in 21 (22.8%) isolates, and serovars 4, 5, 7, and 12 were not detected in any sample.ConclusionThe distribution of 14 Ureaplasma serovars in genital tract of infertile males was identified for the first time by real‐time PCR assay. UPA serovars 1 and 6, and UUR serovar 9 are the most common serovars colonization in urogenital tract of infertile males.

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“…clarithromycin, erythromycin, azithromycin and roxithromycin), but susceptible to 16-membered macrolides (josamycin and miocamycin) and lincomycin [6]. Increasing resistance to fluoroquinolones have been documented in several reports [8,9]. Therefore, rapid detection and susceptibility determination are important to start adequate treatment and to reduce the risk of complications.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Mycoplasma IES, Mycofast Revolution and Mycoplasma IST2 to detect genital mycoplasmas in clinical samples

D’Inzeo

Angelis

Fiori

et al. 2017

J Infect Dev Ctries

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Introduction: Culture is regarded as the gold standard for the detection of genital mycoplasma in clinical samples. Commercially available diagnostic kits, based on liquid broth cultures, provide interesting alternatives to conventional culture. We assessed the laboratory performances of Mycoplasma IES (IES), the Mycofast Revolution (REV) and Mycoplasma IST 2 (IST2) compared to A7 agar plates for the detection of Ureaplasma urealyticum and Mycoplasma hominis in clinical samples. Methodology: From April to July 2013, endocervical or vaginal samples were collected from sexually active women with abnormal vaginal discharge. Each specimen was tested in parallel using the three commercial kits and the A7 agar plates. Results: A total of 303 samples were included in this study, 35.6% (108/303) of which were positive on A7 plates. Sensitivities for the detection of U. urealyticum of IES, REV and IST2 were 100%, 96.2% and 95.3%, respectively while those for M. hominis were of 92.8%, 92.8% and 85.7%, respectively. Specificity was 100% for the 3 methods. Concerning antimicrobial susceptibility testing, full agreement between IES and REV was documented. Conclusions: The Mycoplasma IES kit was found to be equivalent or superior compared to other commercial culture-based assays for a rapid and accurate identification of U. urealyticum and M. hominis and detection of resistance. It might be considered a cost-effective tool for detection of these organisms, particularly attractive in developing countries.

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Epidemiological investigation and antimicrobial susceptibility analysis of ureaplasma species andMycoplasmahominis in outpatients with genital manifestations

Abstract: Our study indicates that high positive rates of Ureaplasma species and M hominis were found in young outpatients with genital symptoms, and monitoring the local drug resistance is critical for prevention of the occurrence of resistant strains.

Cited by 42 publications

References 16 publications

Antibiotic Susceptibility and Sequence Type Distribution of Ureaplasma Species Isolated from Genital Samples in Switzerland

Antibiotic Susceptibility and Sequence Type Distribution of Ureaplasma Species Isolated from Genital Samples in Switzerland

Detection of Ureaplasma spp. serovars in genital tract of infertile males

Comparison of Mycoplasma IES, Mycofast Revolution and Mycoplasma IST2 to detect genital mycoplasmas in clinical samples

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