Epidemiological investigation and drug resistance characteristics of Riemerella anatipestifer strains from large-scale duck farms in Shandong Province, China from March 2020 to March 2022

Lv, Zehao; Han, Shanshan; Li, Jing; Guo, Zhiyun; Geng, Ningwei; Lyu, Chuang; Qin, Liting; Li, Ning

doi:10.1016/j.psj.2023.102759

Cited by 4 publications

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References 35 publications

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“…WGS analysis, which provided in-depth data to understand the AMR in R. anatipestifer , revealed that the aminoglycoside resistance genes ranA and ranB were detected in all isolates, corroborating the findings of previous studies ( 12 ). In addition, 90.64% of the isolates harbored at least one of tet (X2), tet (X4), and tet (X5), which was consistent with previous PCR-based results (80.2%–100%) ( 12 , 14 , 45 , 46 ). Our findings, in contrast to those of other studies ( 12 , 14 ), suggested a high detection rate (93.06%) of the bla OXA -like gene, probably due to the excellent performance of the ARG detection strategy we employed (AMRFinderPlus was employed in combination with high-quality WGS data).…”

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confidence: 91%

Genome-based assessment of antimicrobial resistance reveals the lineage specificity of resistance and resistance gene profiles in Riemerella anatipestifer from China

Yang,

Wang,

Jia

et al. 2024

Microbiol Spectr

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Riemerella anatipestifer ( R. anatipestifer ) is an important pathogen that causes severe systemic infections in domestic ducks, resulting in substantial economic losses for China’s waterfowl industry. Controlling R. anatipestifer with antibiotics is extremely challenging due to its multidrug resistance. Notably, large-scale studies on antimicrobial resistance (AMR) and the corresponding genetic determinants in R. anatipestifer remain scarce. To solve this dilemma, more than 400 nonredundant R. anatipestifer isolates collected from 22 provinces in China between 1994 and 2021 were subjected to broth dilution antibiotic susceptibility assays, and their resistance-associated genetic determinants were characterized by whole-genome sequencing. While over 90% of the isolates was resistant to sulfamethoxazole, kanamycin, gentamicin, ofloxacin, norfloxacin, and trimethoprim, 88.48% of the isolates was resistant to the last-resort drug (tigecycline). Notably, R. anatipestifer resistance to oxacillin, norfloxacin, ofloxacin, and tetracycline was found to increase relatively over time. Genome-wide analysis revealed the alarmingly high prevalence of bla OXA -like (93.05%) and tet (X) (90.64%) genes and the uneven distribution of resistance genes among lineages. Overall, this study reveals a serious AMR situation regarding R. anatipestifer in China, with a high prevalence and high diversity of antimicrobial resistance genes, providing important data for the rational use of antibiotics in veterinary practice. IMPORTANCE Riemerella anatipestifer (R. anatipestifer ), an important waterfowl pathogen, has caused substantial economic losses worldwide, especially in China. Antimicrobial resistance (AMR) is a major challenge in controlling this pathogen. Although a few studies have reported antimicrobial resistance in R. anatipestifer , comprehensive data remain a gap. This study aims to address the lack of information on R. anatipestifer AMR and its genetic basis. By analyzing more than 400 isolates collected over two decades, this study reveals alarming levels of resistance to several antibiotics, including drugs of last resort. The study also revealed the lineage-specificity of resistance profiles and resistance gene profiles. Overall, this study provides new insights and updated data support for understanding AMR and its genetic determinants in R. anatipestifer .

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