Epidemiological patterns of klebsiella colonization and infection in an intensive care ward

Casewell, M.W.; Phillips, Ian

doi:10.1017/s0022172400053651

Cited by 49 publications

(19 citation statements)

References 13 publications

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“…Although this method is tedious, the typability and reproducibility are high. The 80 % typability percentage of our strains does not differ much from that of other authors (Rennie & Duncan, 1974;Casewell & Philips, 1978;Cooke et al 1979; Rennie et al 1978). Serotype 18 was the most common serotype among our strains, a serotype which has, as far as we know, never been reported in the literature as the most prevalent serotype among clinical isolates.…”

Section: Reproducibilitysupporting

confidence: 48%

“…Different methods have been used for capsular serotyping, such as capsular swelling (Casewell, 1972), indirect immunofluorescence (Riser, Noone & Poulton, 1976;Riser, Noone & Bonnet, 1976), counter-current immunoelectrophoresis (Palfreyman, 1978) and coagglutination and latex agglutination (Onokodi & Wauters, 1981). These techniques, ofwhich capsular swelling is the most traditional, have been successfully used by several workers for epidemiological purposes (Casewell & Philips, 1978;Riser, Noone & Thompson, 1978;Seal et al 1981;Smith, Digori & Eng, 1982).…”

Section: Introductionmentioning

confidence: 99%

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Biochemical and serological investigations on clinical isolates of klebsiella

Simoons-Smit¹,

Vught²,

Kanis³

et al. 1985

J. Hyg.

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SUMMARYA series of 925 clinical isolates of klebsiella was examined by serological and biochemical typing. To perform serological typing (capsular swelling) 77 capsular antisera were prepared, tested against the type strains and grouped in 13 pools. With this serotyping method 800 ofthe cultures were typable and 63 distinct types could be recognized.All strains were typable biochemically by means of the numerical coding system of the API-20E system supplemented by digits derived from 15 additional conventional biochemical tests. With the API-20E system 24 different biotypes could be distinguished whereas the combination of API-20E and the 15 additional tests produced 93 biotypes. Maximum discrimination of strains was achieved by the combination of serological and biochemical typing (256 bioserotypes). The reproducibility, typability and discriminating power of the biotyping system was not inferior to serotyping. For epidemiological purposes biotyping can replace serotyping of Klebsiella species, especially in laboratories less well equipped.

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Section: Reproducibilitysupporting

confidence: 48%

Section: Introductionmentioning

confidence: 99%

Biochemical and serological investigations on clinical isolates of klebsiella

Simoons-Smit¹,

Vught²,

Kanis³

et al. 1985

J. Hyg.

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“…This clustering occurred in the neonatal ward with serotypes or biotypes which were not found to be particularly common in other wards of the hospital. This predominance of a known klebsiella type for a short period of time and its replacement by another klebsiella type is in agreement with the findings of Casewell & Phillips (1978). K 18, K55 and K69 were the commonest serotypes in the neonatal ward during this study.…”

Section: Discussionsupporting

confidence: 92%

“…Nevertheless it has been used successfully in the epidemiological investigations of infections with Klebsiella spp. (Casewell & Phillips, 1978;Casewell & Talsania, 1979;Cooke et al 1979;Riser, Noone & Howard, 1980).…”

Section: Introductionmentioning

confidence: 99%

Comparison of biochemical and serological typing results and antimicrobial susceptibility patterns in the epidemiological investigation of Klebsiella spp

et al. 1987

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SUMMARYAn analysis of the serological and biochemical typing results of 925 clinical isolates of klebsiella revealed that biotyping and serotyping of klebsiella could replace each other for epidemiological purposes. The combination of both typing methods provided even more epidemiological information in analysing clusters of particular serotypes and biotypes in time. Clustering serotypes, mainly of neonatal origin, were nearly uniformly more resistant to the antibiotics in common use than other serotypes. Biotyping as well as serotyping of klebsiella isolates recovered from environmental surveys in the neonatal ward showed that epidemic and non-epidemic klebsiella isolates could occasionally be cultured from the environment and from the staff.

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“…There is little information on the stability of klebsiella carriage in the human faecal flora (Montgomerie et al, 1970, Casewell andPhillips, 1978a). However, these reports, our limited studies in geriatric patients, and experience of other authors with Escherichia coli suggest that unusual frequency of sampling of active and inactive disease could distort the apparent frequency of klebsiella carriage if 1 positive out of a number of cultures is considered to imply positivity throughout a 3-month period.…”

mentioning

confidence: 47%