Epidemiological Studies of Hydatidosis Among Slaughtered Sheep and Human in Taif, Saudi Arabia

Al-Malki, Jamila S.; Degheidy, Nabila S.

doi:10.21608/avmj.2013.171910

Cited by 3 publications

(1 citation statement)

References 8 publications

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“…In the current study, the incidence of cystic hydatidosis among sheep (0.43%) was the lowest among those reported in Saudi Arabia, including 1.06-2.33% in Riyadh (Almalki et al, 2017;Abdel-Baki et al, 2018), 12.61% in Al Baha (Ibrahim, 2010), 69.6% in Jeddah (Toulah et al, 2012), 9.6-13.5% in Al-Taif (Al-Malki andDegheidy, 2013;Hayajneh et al, 2014;Al Malki and Ahmed, 2022), 6.6.% in the Al-Ahsa region (El-Ghareeb et al, 2017), 9.6% in Al-Taif (Al-Malki and Degheidy, 2013), 6.8% in Najran (Almalki et al, 2017), 7.06% in Hail (Hasona et al, 2017) and 2.83% in Dammam (El-Ghareeb et al, 2017). There are several explanations for the differences in disease prevalence in different regions of Saudi Arabia, including different weather (rainfall and temperature) patterns, the timeframe in which the study was conducted, and the abundance of infected host dogs in each area (Hasona et al, 2017).…”

Section: Discussionmentioning

confidence: 45%

Epidemiology, Molecular, and Phylogenetic Characterization of Echinococcus granulosus Cysts in Slaughtered Farm Animals in Al-Jouf Province, Saudi Arabia

Alkhaldi

2023

WVJ

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Echinococcosis, or hydatidosis, is a serious veterinary disease and public health issue worldwide, particularly in rural areas in which dogs have frequent contact with local herbivores. This study assessed the frequency of hydatidosis found among farm animals slaughtered in Al-Jouf Province in northern Saudi Arabia in 2021. A total of 156754 sheep, 36337 goats, 8590 camels, and 986 cattle were inspected for hydatidosis infection by comprehensive evaluation involving meticulous visual inspection and manual exploration of the internal organs through palpation. The cysts were subjected to molecular and phylogenetic analysis. The overall prevalence rates of hydatid cysts were 0.43%, 0.19%, 0.54%, and 0.51% in the inspected sheep, goats, camels, and cattle, respectively. The highest disease prevalence rates among sheep (27.8%) and goats (30.9%) occurred in the spring, and the highest prevalence rates among camels (41.3%) and cattle (80%) were in the summer. Regarding the prevalence of the disease in four slaughterhouses in the Al-Jouf Province, the highest prevalence in sheep, goats, and camels was in the Tabarjal slaughterhouse (1.43%, 0.81%, and 1.08%, respectively), although the Al-Qurayat slaughterhouse had the highest prevalence rate in cattle (1.98%). Complete molecular analysis indicated that cytochrome c oxidase subunit 1 (cox1) sequences from cyst isolates belonged to Echinococcus granulosus (E. granulosus). Moreover, there was high homology (98-100%) with associated Genbank sequences of E. granulosus isolated from sheep in the Kingdom of Saudi Arabia (KSA). Sheep and camels were a significant source of hydatidosis transmission to dogs and helped to maintain disease incidence in the Al-Jouf Province. Thus, significant efforts should focus on preventing cyst transmission from abattoirs and infected stray dogs.

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Section: Discussionmentioning

confidence: 45%

Epidemiology, Molecular, and Phylogenetic Characterization of Echinococcus granulosus Cysts in Slaughtered Farm Animals in Al-Jouf Province, Saudi Arabia

Alkhaldi

2023

WVJ

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Three species of Echinococcus granulosus sensu lato infect camels on the Arabian Peninsula

Al-Hizab

Mohamed²,

Wassermann

et al. 2021

Parasitol Res

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We report on the genetic identity of 36 Echinococcus cysts that were collected during a recent slaughterhouse survey of 810 locally bred camels (dromedaries) in the Eastern Province of the Kingdom of Saudi Arabia. Analysis of a partial nad1 gene sequence showed that the majority (n = 29) belonged to E. granulosus sensu stricto, four to E. canadensis G6/7, and three to E. ortleppi. Eight of the 29 E. granulosus s.s. cysts contained protoscoleces; all other cysts were calcified and non-viable. This is the first report of the presence E. ortleppi from the Arabian Peninsula, a parasite that is typically transmitted via cattle. The results indicate widespread infection of camels with CE in eastern Saudi Arabia and an active role of camels in the lifecycles of at least E. granulosus s.s.. Complete cox1 haplotype analysis of 21 E. granulosus s.s. isolates shows that the majority of variants circulating in eastern Saudi Arabia is distinct from but closely related to haplotypes from neighboring countries in the Middle East, which indicates the presence of this parasite in KSA for a longer period of time. All isolates of E. granulosus s.s. in this study belonged to the G1 cluster, although the G3 genotype has previously also been reported from the Middle East.

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Molecular characterization ofEchinococcus granulosusin livestock of Al-Madinah (Saudi Arabia)

2020

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Echinococcus granulosus is the causative agent of cystic echinococcosis, which has serious impacts on human and/or animal health, resulting in significant economic losses. Echinococcus granulosus comprises a number of intra-specific variants or strains at the genetic level. In Saudi Arabia, few studies were performed on genetic variations in Echinococcus species. Therefore, the present study aimed to investigate the phenotypic and genetic characterization of hydatid cysts harboured by sheep and camels in Al-Madinah Al-Munawarah. Samples of hydatid cysts were collected from local sheep (n = 25) and camels (n = 8). The morphological criteria of protoscoleces were investigated. To investigate the molecular characterization, random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR), single-stranded conformation polymorphism (SSCP) were carried out. DNA was extracted from individual fertile cysts and subjected to RAPD-PCR analysis (using five arbitrary primers) and PCR amplification of cytochrome c oxidase I (cox1) and 12S ribosomal ribonucleic acid (12S rRNA) genes. The PCR products were subjected to SSCP analysis for genetic discrimination in E. granulosus isolates. In addition, partially sequencing of the mitochondrial DNA cox1 genes was achieved for assessing the phylogenetic positions of collected isolates using some global published sequence data of cox1 genes. The rostellar hooks of camel and local sheep isolates show remarkable variability in their dimensions. Five distinct SSCP patterns were identified in the 12S rRNA gene, showing intraspecific variations in E. granulosus of camels and local sheep. Sequencing of (cox1) genes of both local sheep and camels exhibit high similarity with those of the same gene (E. granulosus sensu stricto) published in NCBI BLAST.

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Epidemiological Studies of Hydatidosis Among Slaughtered Sheep and Human in Taif, Saudi Arabia

Cited by 3 publications

References 8 publications

Epidemiology, Molecular, and Phylogenetic Characterization of Echinococcus granulosus Cysts in Slaughtered Farm Animals in Al-Jouf Province, Saudi Arabia

Epidemiology, Molecular, and Phylogenetic Characterization of Echinococcus granulosus Cysts in Slaughtered Farm Animals in Al-Jouf Province, Saudi Arabia

Three species of Echinococcus granulosus sensu lato infect camels on the Arabian Peninsula

Molecular characterization ofEchinococcus granulosusin livestock of Al-Madinah (Saudi Arabia)

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