Epidemiological study of Mycoplasma pneumoniae infections in japan based on PCR-restriction fragment length polymorphism of the P1 cytadhesin gene

Sasaki, Tsuguo; Kenri, Tsuyoshi; Okazaki, Norio; Iseki, Mikirou; Yamashita, Ryoko; Shintani, Miharu; Sasaki, Yuko; Yayoshi, Masumi

doi:10.1128/jcm.34.2.447-449.1996

Cited by 89 publications

(51 citation statements)

References 9 publications

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“…A recent retrospective genotyping analysis of isolates collected over a 10-year period by Kenri et al [11] provides evidence of the phenomenon of type-switching occurring approximately every 7 years in Japan. Sasaki et al [10] had previously documented distinct shifts in M. pneumoniae types over a 20-year period in Japan, with intervals of 4 years between epidemics; however, they were unable to correlate this shift definitively with epidemic cycles. Type-switching has been proposed to occur due to population-based immune pressure and may explain the cyclic nature of both type-specific outbreaks and subsequent immunity [22].…”

Section: Discussionmentioning

confidence: 94%

“…Methods for differentiating isolates may serve numerous purposes. For instance, monitoring epidemiological trends of M. pneumoniae outbreaks may lead to greater understanding 4,5,6,7,10,11,12) and type of the biology and communicable nature of this agent and yield insight into its ability to cause cyclic epidemics. A recent retrospective genotyping analysis of isolates collected over a 10-year period by Kenri et al [11] provides evidence of the phenomenon of type-switching occurring approximately every 7 years in Japan.…”

Section: Discussionmentioning

confidence: 99%

“…Currently, the standard typing method for distinguishing M. pneumoniae isolates involves PCR-restriction fragment length polymorphism (PCR-RFLP), which can be laborious and time-consuming [10][11][12][13]. Recently, Dumke et al [14] described a nested-PCR followed by sequencing that can be used directly with clinical specimen extracts to distinguish each type and all known variants of M. pneumoniae.…”

Section: Introductionmentioning

confidence: 99%

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Genotyping of Mycoplasma pneumoniae isolates using real-time PCR and high-resolution melt analysis

Schwartz

Thurman

Mitchell

et al. 2009

Clinical Microbiology and Infection

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Mycoplasma pneumoniae is an important respiratory pathogen, accounting for up to 25% of community-acquired pneumonia, and is a common cause of hospitalized pneumonia in otherwise healthy adults and children. Mycoplasma pneumoniae isolates can be classified into two main genomic groups (type 1 and type 2) based on sequence variation within the gene encoding the major adhesion molecule P1. Although numerous publications have described real-time PCR assays for the detection of M. pneumoniae, none has been able to discriminate the two genomic types. Here, a real-time PCR assay that can distinguish each type of M. pneumoniae utilizing high-resolution melt-curve analysis is reported. Using this method, 102 isolates obtained from patients from 1965 to the present, including those from recent outbreaks, were typed along with reference strains M129 (type 1) and FH (type 2). The results show that 55 isolates (54%) can be classified as type 1 and 47 isolates (46%) as type 2, and 100% correlation was demonstrated when compared with a standard PCR-restriction fragment length polymorphism typing procedure. Typing of isolates obtained from recent outbreaks in the USA has revealed the presence of both types. This assay provides a rapid, reliable and convenient method for typing M. pneumoniae isolates and may be useful for surveillance purposes and epidemiological investigations, and may provide insight into the biology of M. pneumoniae distribution within populations.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genotyping of Mycoplasma pneumoniae isolates using real-time PCR and high-resolution melt analysis

Schwartz

Thurman

Mitchell

et al. 2009

Clinical Microbiology and Infection

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“…Comprehensive analyses of recent outbreaks together may reveal trends in circulating M. pneumoniae types that are masked by the limited specimen collection in individual outbreak scenarios. Previous studies have reported alternating dominance of P1 types in 3-to 7-year cycles (23,24), a phenomenon that may be driven by the development of temporary immunity to the dominant type within a population. In support of this observation, no type 1 clinical isolates were recovered from 1995 to 2007 (n ϭ 60) in any CDC-investigated U.S. outbreak.…”

Section: Discussionmentioning

confidence: 97%

Detection and Characterization of Mycoplasma pneumoniae during an Outbreak of Respiratory Illness at a University

et al. 2014

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An outbreak at a university in Georgia was identified after 83 cases of probable pneumonia were reported among students. Respiratory specimens were obtained from 21 students for the outbreak investigation. The TaqMan array card (TAC), a quantitative PCR (qPCR)-based multipathogen detection technology, was used to initially identify Mycoplasma pneumoniae as the causative agent in this outbreak. TAC demonstrated 100% diagnostic specificity and sensitivity compared to those of the multiplex qPCR assay for this agent. All M. pneumoniae specimens (n ‫؍‬ 12) and isolates (n ‫؍‬ 10) were found through genetic analysis to be susceptible to macrolide antibiotics. The strain diversity of M. pneumoniae associated with this outbreak setting was identified using a variety of molecular typing procedures, resulting in two P1 genotypes (types 1 [60%] and 2 [40%]) and seven different multilocus variable-number tandem-repeat analysis (MLVA) profiles. Continued molecular typing of this organism, particularly during outbreaks, may enhance the current understanding of the epidemiology of M. pneumoniae and may ultimately lead to a more effective public health response.

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“…Various DNA-based methods have been used for intraspecies differentiation of mycoplasma isolates. They include restriction endonuclease analysis (27), field inversion gel electrophoresis (12), restriction fragment length polymorphism (8), PCR-mediated restriction fragment length polymorphism (34), arbitrarily primed PCR (15), and insertion sequence typing (6). However, most of the genetic typing assays also have drawbacks in that they may require a relatively large amount of high-quality DNA or may be difficult to reproduce and standardize between laboratories.…”

mentioning

confidence: 99%

Amplified-Fragment Length Polymorphism Fingerprinting of Mycoplasma Species

Kokotovic

Friis²,

Jensen

et al. 1999

J Clin Microbiol

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Amplified-fragment length polymorphism (AFLP) is a whole-genome fingerprinting method based on selective amplification of restriction fragments. The potential of the method for the characterization of mycoplasmas was investigated in a total of 50 strains of human and animal origin, including Mycoplasma genitalium(n = 11), Mycoplasma pneumoniae(n = 5), Mycoplasma hominis(n = 5), Mycoplasma hyopneumoniae(n = 9), Myco plasma flocculare(n = 5), Mycoplasma hyosynoviae(n = 10), and Mycoplasma dispar(n = 5). AFLP templates were prepared by the digestion of mycoplasmal DNA with BglII and MfeI restriction endonucleases and subsequent ligation of corresponding site-specific adapters. The amplification of AFLP templates with a single set of nonselective primers resulted in reproducible fingerprints of approximately 60 to 80 fragments in the size range of 50 to 500 bp. The method was able to discriminate the analyzed strains at species and intraspecies levels as well. Each of the testedMycoplasma species developed a banding pattern entirely different from those obtained from other species under analysis. Subtle intraspecies genomic differences were detected among strains of all of the Mycoplasma species analyzed. The extent of polymorphism varied markedly between the analyzed mycoplasmas, comprising pattern similarity levels from 61.7% detected among M. disparstrains to 95.9% detected among M. genitalium strains. The results of the present study provide evidence of the high discriminatory power of AFLP analysis, suggesting the possible applicability of this method to the molecular characterization of mycoplasmas.

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Epidemiological study of Mycoplasma pneumoniae infections in japan based on PCR-restriction fragment length polymorphism of the P1 cytadhesin gene

Cited by 89 publications

References 9 publications

Genotyping of Mycoplasma pneumoniae isolates using real-time PCR and high-resolution melt analysis

Genotyping of Mycoplasma pneumoniae isolates using real-time PCR and high-resolution melt analysis

Detection and Characterization of Mycoplasma pneumoniae during an Outbreak of Respiratory Illness at a University

Amplified-Fragment Length Polymorphism Fingerprinting of Mycoplasma Species

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