Epidemiological study on human and canine leptospirosis in Central and North Kerala

Soman, Manju; Jayaprakasan, V.; Mini, M.

doi:10.14202/vetworld.2014.759-764

Cited by 5 publications

(4 citation statements)

References 7 publications

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“…[ 26 ], they reported 2.6%. Since agglutinins to MAT tend to remain in the body for a prolonged period following infection, detectable MAT titers may be present even in healthy animals [ 27 ]. Sera samples were collected from 78 healthy dogs.…”

Section: Resultsmentioning

confidence: 99%

Development of indirect enzyme-linked immunosorbent assay for diagnosis of canine leptospirosis

et al. 2017

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Aim:This study was taken up to develop an indirect enzyme-linked immunosorbent assay (i-ELISA) for screening antibodies against Leptospira spp. in canines.Materials and Methods:An i-ELISA was developed using outer membrane protein extracted from Leptospira interrogans serovar canicola used for coating the well with concentration of 0.5 µg/µl. A total of 250 serum samples from clinically affected and apparently healthy dogs were collected along with relevant epidemiological data at Teaching Veterinary Clinical Complex, Veterinary College and Research Institute, Namakkal, and subjected to i-ELISA.Results:Out of 250 sera samples, 140 (56.00%) were found to be positive by i-ELISA. All the sera samples were subjected to microagglutination test (MAT) with panel of 12 different serovars. A total of 71 (28.40%) sera samples were positivity to MAT excluding the sera samples positive to L. interrogans serovars canicola and icterohaemorrhagiae in vaccinated dogs. Sensitivity and specificity of i-ELISA were higher in compared with MAT was 91.54% and 58.10%, respectively.Conclusion:An indirect ELISA developed for the detection of canine antileptospiral antibodies proved to be highly sensitive, rapid and easy to perform and overcome the drawbacks of MAT.

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Section: Resultsmentioning

confidence: 99%

Development of indirect enzyme-linked immunosorbent assay for diagnosis of canine leptospirosis

et al. 2017

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“…In our study, we detected only one positive serum from canine clinical case for serovar Grippotyphosa. Serological and isolation studies in canine species from Indian state of Kerala revealed 36.36% seropositivity with Australis and Pomona as the prevalent serovars (Soman et al, 2014). Patil et al,…”

Section: Resultsmentioning

confidence: 98%

Seroepidemiology of Leptospirosis in Domestic Animals from India

Inbaraj¹,

Singh²,

Chaudhuri³

2019

Int.J.Curr.Microbiol.App.Sci

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“…AMBILY et al, (2013) observed Leptospira interrogans serovar Autumnalis as the most prevalent serovar among dogs in Kerala, followed by Australis, Pomona, Canicola, Pyrogens, Icterohaemorrhagiae, Javanica and Patoc. The study by SOMAN et al (2014) identified the serovars Pomona, and Australis as common ones infecting humans, animals, and rodents, in central and North Kerala, by serology and isolation.…”

Section: Resultsmentioning

confidence: 99%

Seroprevalence of leptospirosis among captive Asian elephants in Kerala, India - a short communication

Varied Tresamol,

Amel Dev,

Ranganath Amulya

et al. 2023

Vet. Arh

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Leptospirosis is an infectious disease of zoonotic significance, caused by distinct members of the genus Leptospira, with a broad spectrum of hosts, including domestic and wild animals. The present paper reports the seroprevalence of leptospirosis among captive Asian elephants in Kerala. Whole blood samples were collected from fifty, apparently healthy captive elephants from different districts of Kerala state. The serum was separated from the whole blood and subjected to the Microscopic Agglutination Test (MAT) for detection of antibodies to different serovars of Leptospira. The test was carried out using actively grown cultures of twelve common leptospiral serovars. Out of the total of fifty serum samples screened by MAT, 19 samples (38 per cent) showed a positive reaction with antibody titres in a 1:200 dilution. The prevalent serovars were Pomona, Icterohaemorrhagiae, Hebdomadis, Grippotyphosa and Canicola, and the highest prevalence was noticed for the serovar Pomona. The positive results indicated the presence of infection or the previous exposure of captive elephants to leptospiral antigens. The high rate of prevalence of leptospiral antibodies in captive elephants warrants the monitoring of these animals for clinical disease and adopting the necessary control strategies for preventing this re-emerging zoonotic disease.

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Epidemiological study on human and canine leptospirosis in Central and North Kerala

Cited by 5 publications

References 7 publications

Development of indirect enzyme-linked immunosorbent assay for diagnosis of canine leptospirosis

Development of indirect enzyme-linked immunosorbent assay for diagnosis of canine leptospirosis

Seroepidemiology of Leptospirosis in Domestic Animals from India

Seroprevalence of leptospirosis among captive Asian elephants in Kerala, India - a short communication

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