Epidemiological surveillance of colonising group B Streptococcus epidemiology in the Lisbon and Tagus Valley regions, Portugal (2005 to 2012): emergence of a new epidemic type IV/clonal complex 17 clone

Florindo, Catarina; Damião, Vera; Silvestre, Inês; Farinha, Carla Sofia e Sá; Rodrigues, F; Nogueira, Fátima; Martins-Pereira, F; Castro, Rogério; Borrego, Maria José; Santos-Sanches, Ilda

doi:10.2807/1560-7917.es2014.19.23.20825

Cited by 39 publications

(36 citation statements)

References 27 publications

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“…The most common GBS CPSs seen in Alberta over the 11-year surveillance period were CPSs III, V, and Ia. This is not unusual, as CPS III has been documented to be the most common CPS identified among cases of invasive disease and in carriage in studies elsewhere (Toronto, Beijing, Ireland, England, Portugal, and globally) (9,15,(29)(30)(31)(32). CPS III accounted for one-quarter to one-half of the total GBS isolates serotyped in past epidemiological surveys.…”

Section: Cps Distributions and Incidence Ratesmentioning

confidence: 89%

“…A major hypervirulent MLST clone currently circulating globally is clonal complex 17 (CC17) (9,(13)(14)(15)(16). This lineage is strongly associated with the majority of GBS LOD infections, which are characterized by meningitis in infants after the first week of life (13).…”

mentioning

confidence: 99%

“…This lineage is strongly associated with the majority of GBS LOD infections, which are characterized by meningitis in infants after the first week of life (13). CC17 clones possess a hypervirulent GBS adhesin gene, hvgA, which is usually present in CPS III strains (9,(13)(14)(15)(16). There have been reports of CPS IV isolates that contain CC17-specific hvgA (9); therefore, the PCR amplification of hvgA that had been used to assign GBS strains to CC17 (16) was found to be inconclusive (9).…”

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confidence: 99%

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Epidemiology of Invasive Group B Streptococcal Disease in Alberta, Canada, from 2003 to 2013

2016

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bGroup B streptococci (GBS) cause severe invasive disease in both neonates and adults. Understanding the epidemiology of GBS provides information that can include determining disease prevalence rates in defined populations and geographic regions, documenting the success of GBS screening programs, and understanding antimicrobial susceptibility patterns. In Alberta, only neonatal invasive GBS (iGBS) disease is notifiable to health authorities. We performed a surveillance study of iGBS in Alberta, Canada, from 2003 to 2013. Over the 11-year period, the disease incidence rate increased from a low of 3.92 cases/100,000 population to a high of 5.99 cases/100,000 population. Clindamycin resistance also increased, from 12.2% to 32.5%. In summary, Alberta, Canada, has experienced an increase in GBS disease; the increase includes both neonatal and adult disease. CPS IV cases also notably increased during the surveillance period, as did resistance to erythromycin and clindamycin.

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Section: Cps Distributions and Incidence Ratesmentioning

confidence: 89%

mentioning

confidence: 99%

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confidence: 99%

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Epidemiology of Invasive Group B Streptococcal Disease in Alberta, Canada, from 2003 to 2013

2016

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“…Frequencies of less than 1% were common in the 1990s (46) but have increased to 6 to 12% in populations studied between 2008 and 2015 (27,29,30,47). The emergence of serotype IV GBS among colonizing isolates has also been noticed in the United States (28) and Europe (48). In this study, we found that serotype IV strains accounted for 6% of the colonizing GBS isolates recovered in metropolitan Toronto.…”

Section: Discussionmentioning

confidence: 64%

Serotype Distribution, Population Structure, and Antimicrobial Resistance of Group B Streptococcus Strains Recovered from Colonized Pregnant Women

Teatero

Ferrieri

Martín³

et al. 2017

J Clin Microbiol

103

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Using serotyping, multilocus sequence typing, and whole-genome sequencing (WGS) of selected strains, we studied the population structure of 102 group B Streptococcus (GBS) isolates prospectively sampled in 2014 from vaginal/ rectal swabs of healthy pregnant women in metropolitan Toronto, Canada. We also determined the susceptibilities of each of the colonizing isolates to penicillin, erythromycin, clindamycin, tetracycline, and other antimicrobial agents. Overall, we observed a high rate of tetracycline resistance (89%) among colonizing GBS isolates. We found resistance to erythromycin in 36% of the strains, and 33% were constitutively or inducibly resistant to clindamycin. The most frequently identified serotypes were III (25%), Ia (23%), and V (19%). Serotype IV accounted for 6% of the colonizing isolates, a rate consistent with that observed among patients with invasive GBS infections in metropolitan Toronto. The majority of serotype IV isolates belonged to sequence type (ST)459, a tetracycline-, erythromycin-, and clindamycinresistant ST first identified in Minnesota, which is considered to be the main driver of serotype IV GBS expansion in North America. WGS revealed that ST459 isolates from Canada are clonally related to colonizing and invasive ST459 organisms circulating in regions of the United States. We also used WGS to study recombination in selected colonizing strains from metropolitan Toronto, which revealed multiple episodes of capsular switching. Present and future circulating GBS organisms and their genetic diversity may influence GBS vaccine development.

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“…An important virulence factor of GBS is a capsular polysaccharide (CPS); there are ten antigenic CPS variants designated Ia, Ib, and II to IX (8)(9)(10)(11). In North America and a number of European countries, five CPS types (Ia, Ib, II, III, and V) cause the bulk of invasive disease cases, with CPS III causing a higher rate of disease among neonates and CPS types Ia and V causing higher rates among adult patients (12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23). Interestingly, recent studies have noted the emergence and circulation of CPS IV, a previously uncommon serotype, as an important cause of both neonatal and adult infections (14,19,(24)(25)(26)(27)(28).…”

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confidence: 99%

Identification of Group B Streptococcus Capsule Type by Use of a Dual Phenotypic/Genotypic Assay

2017

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The group B streptococcus (GBS) capsular polysaccharide (CPS) is an important virulence factor which is also used for GBS typing. There are 10 CPS types (Ia, Ib, and II to IX). GBS that do not phenotypically type are considered nontypeable. All genes required for CPS synthesis are found on the GBS cps operon, which contains a highly variable CPS-determining region (cpsG-cpsK). The objective of this study was development of an assay to detect sialic acid on the GBS cell surface, followed by a genotypic PCR CPS typing assay. Sialic acid is located at the terminal end of the side chain of all known GBS CPS types. Sialic acid can be bound to commercially available lectins such as slug Limax flavus lectin. Biotinylated L. flavusstreptavidin-peroxidase complex was used in an enzyme immunoassay and dot blot assay to detect sialic acid. This was followed by a PCR typing scheme that was developed to target the serotype-determining region of the cps locus for Ia, Ib, and II to IX. Sialic acid from the CPS types Ia, Ib, and II to IX was detectable on the GBS cell surfaces of all previously identified CPS-typed GBS strains assayed. This was followed by the real-time PCR typing assay which successfully identified CPS Ia, Ib, and II to IX types. The combination of phenotypic and genotypic assays provides an accurate tool for detection of CPS expression and assignment of CPS typing. These assays have the potential to be used for CPS typing in large-scale epidemiological studies.

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Epidemiological surveillance of colonising group B Streptococcus epidemiology in the Lisbon and Tagus Valley regions, Portugal (2005 to 2012): emergence of a new epidemic type IV/clonal complex 17 clone

Cited by 39 publications

References 27 publications

Epidemiology of Invasive Group B Streptococcal Disease in Alberta, Canada, from 2003 to 2013

Epidemiology of Invasive Group B Streptococcal Disease in Alberta, Canada, from 2003 to 2013

Serotype Distribution, Population Structure, and Antimicrobial Resistance of Group B Streptococcus Strains Recovered from Colonized Pregnant Women

Identification of Group B Streptococcus Capsule Type by Use of a Dual Phenotypic/Genotypic Assay

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