Epidemiological survey and molecular characterization of Echinococcus granulosus in cattle in an endemic area of eastern Turkey

Şimşek, Sami; Balkaya, İbrahim; Köroğlu, Ergün

doi:10.1016/j.vetpar.2010.05.016

Cited by 38 publications

(27 citation statements)

References 12 publications

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“…The investigators used different methods to classify E. granulosus isolates from different hosts in Turkey, such as PCR-RFLP of the ITS-1 region of the rRNA gene and sequencing of CO1 (15); comparative sequencing of the CO1 and/or ND1 genes (12)(13)(14); specific PCR analysis of the 12S rRNA gene and sequencing of CO1 (16,18); and specific PCR analysis of the 12S rRNA gene. Another study performed SSCP analysis of the CO1 gene and sequencing of CO1 (17).…”

Section: Discussionmentioning

confidence: 99%

“…According to these reports, G1 was the most prevalent strain among human and animal isolates. The G1 genotype has been demonstrated in humans, sheep, goats, cattle, camels, water buffaloes and dogs (12)(13)(14)(15)(16); the G3 genotype has been demonstrated in humans, sheep and cattle (12,13); and the G7 genotype has been demonstrated in humans and sheep (13), whereas G6 has only been identified in humans (18).…”

Section: Discussionmentioning

confidence: 99%

“…Although there are some molecular studies of E. granulosus isolates originating from different hosts in Turkey (12)(13)(14)(15)(16)(17)(18), no mitochondrial DNA sequence data exists for Echinococcus species from the European part of Turkey.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization of Human and Animal Isolates of Echinococcus granulosus in the Thrace Region, Turkey

Eryıldız¹,

Şakru²

2012

Balkan Med J

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Molecular Characterization of Human and Animal Isolates of Echinococcus granulosus in the Thrace Region, Turkey

Eryıldız¹,

Şakru²

2012

Balkan Med J

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“…Parazitin arakonaklarda şekillendirdiği larva formunun adı hidatid kist, oluşturduğu hastalık ise "kistik echinococcosis" olarak adlandırılmaktadır (8). Dünyanın pek çok bölgesinde farklı arakonaklarda varlığı bilinen bu parazit (8,24), Türkiye'de gerek çiftlik hayvanlarında gerekse insanlarda sıkça rapor edilmektedir (5,17,20,28,33).…”

Section: Introductionunclassified

Kırıkkale’de kesilen sığırlarda Echinococcus granulosus’un moleküler olarak genotiplendirilmesi

DEĞİRMENCİ¹

2017

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Özet: Echinococcus granulosus türü içindeki suş farklılıkları etkenin konak spesifitesi, gelişim hızı, patojenitesi, antijenik özel-likleri, kemoterapotiklere duyarlılığı, bulaşma dinamikleri ve epidemiyolojide farklılıklar şekillendirmektedir. Bu çalışmada; Kırıkkale Yöresi'nde kesilen sığırların karaciğer ve akciğerlerinden elde edilen E. granulosus izolatlarının suş identifikasyonu yapılması amaç-lanmıştır. Bunun için parazitin 12S rRNA ve mitokondriyal cytochrome c oxidase subunit 1 (COI) geni spesifik primerlerle çoğaltıl-mıştır ve COI geni sekanslatılmıştır. COI sekans sonucuna göre, elde edilen izolatların G1-G3 kompleksinde olduğu belirlenmiştir.Anahtar sözcükler: Echinococcus granulosus, genotiplendirme, hidatid kist, Kırıkkale, sığır. Genotyping of Echinococcus granulosus obtained from cattle slaughtered in Kırıkkale ProvinceSummary: Strain differences are responsible from some important differences such as host specifity, growth rate, antigenic properties, sensitivity to chemotherapeutic agents, transmission dynamics and epidemiological variations in Echinococcus granulosus. In this study, it was aimed to detection strain identification of E. granulosus obtained from liver and lungs of cattle slaughtered in Kırıkkale region. For this aim, mitochondrial cytochrome c oxidase subunit 1 (COI) and 12S rRNA genes of E. granulosus isolates amplified by specific primers and then COI gene sequence was made. In the present study, the isolates were identified as G1-G3 complex according to COI sequencing results.

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“…To date, only two species of Echinococcus have been reported in both human and livestock in Turkey, E. granulosus sensu stricto (G1-G3 complex) and E. canadensis (G6 and G7 genotypes) (Utuk et al, 2008;Snabel et al, 2009;Simsek et al, 2010;Simsek et al, 2011b). Molecular characterization of E.granulosus in a horse isolate in Turkey, was firstly reported by Utuk and Simsek, 2013. It was reported that the equids are intermediate hosts for E. equinus (Jenkins et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

First detection and molecular characterization of Echinococcus equinus in a Mule in Turkey

Şimşek

Çevik

2014

Acta Parasitologica

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Cystic echinococcosis is a zoonotic disease with a cosmopolital distribution. It is caused by the larval stages (metacestodes) of the parasite Echinococcus granulosus which infects different animal species. In this report, we present a case of E. granulosus infection in a mule and molecular characterization of the cyst. For this purpose parasite material was collected from the liver of a necropsied mule. DNA was isolated and PCR amplification of mitochondrial 12S rRNA as well as partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) genes were performed. Six unilocular cysts, filled with clear fluid were found in the liver and spleen. All cysts were found to be fertile. The 12S rRNA-PCR did not yield any band while mt-CO1-PCR yielded a 446 bp sized amplification product. Sequence corresponding to mt-CO1 gene was identical to a sequence reported for E. equinus (formerly G4) (Genbank accession number: KC953029). This is the first record of E. equinus as a cause of cystic echinococcosis in a mule in Turkey.

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Epidemiological survey and molecular characterization of Echinococcus granulosus in cattle in an endemic area of eastern Turkey

Cited by 38 publications

References 12 publications

Molecular Characterization of Human and Animal Isolates of Echinococcus granulosus in the Thrace Region, Turkey

Molecular Characterization of Human and Animal Isolates of Echinococcus granulosus in the Thrace Region, Turkey

Kırıkkale’de kesilen sığırlarda Echinococcus granulosus’un moleküler olarak genotiplendirilmesi

First detection and molecular characterization of Echinococcus equinus in a Mule in Turkey

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